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Copper-Containing Anti-Biofilm Nanofiber Scaffolds as a Wound Dressing Material.

Ahire JJ, Hattingh M, Neveling DP, Dicks LM - PLoS ONE (2016)

Bottom Line: Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter).The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC).After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.

ABSTRACT
Copper particles were incorporated into nanofibers during the electrospinning of poly-D,L-lactide (PDLLA) and poly(ethylene oxide) (PEO). The ability of the nanofibers to prevent Pseudomonas aeruginosa PA01 and Staphylococcus aureus (strain Xen 30) to form biofilms was tested. Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter). The crystalline structure of the copper particles in Cu-F was confirmed by X-ray diffraction (XRD). Copper crystals were encapsulated, but also attached to the surface of Cu-F, as shown scanning transmission electron microscopy (STEM) and transmission electron microscopy (TEM), respectively. The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC). After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively. Reduction in biofilm formation was ascribed to copper released from the nanofibers. Copper-containing nanofibers may be incorporated into wound dressings.

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SEM-energy-dispersive X-ray (EDX) analysis of (a) nanofibers without copper (CF), (b) copper-containing nanofibers (Cu-F) and (c) copper particles. The EDX scan spectra of selected areas pointed out in images (a), (b) and (c) are shown in (d), (e) and (f), respectively. The percentages shown for each of the elements are the mean of three selected areas from SEM images.
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pone.0152755.g002: SEM-energy-dispersive X-ray (EDX) analysis of (a) nanofibers without copper (CF), (b) copper-containing nanofibers (Cu-F) and (c) copper particles. The EDX scan spectra of selected areas pointed out in images (a), (b) and (c) are shown in (d), (e) and (f), respectively. The percentages shown for each of the elements are the mean of three selected areas from SEM images.

Mentions: Analyses with SEM-energy-dispersive X-ray (EDX) of Cu-F (Fig 2B) and copper particles (Fig 2C) revealed the presence of C, O, Cu, and Zn (Fig 2E and 2F). In addition, trace quantities of Al (0.43 ± 0.41 wt. %) were also detected in copper particles (Fig 2C and 2F). Analyses of CF showed C and O as major elements, with small quantities (0.45 ± 0.12 wt. %) of Si (Fig 2A and 2D).


Copper-Containing Anti-Biofilm Nanofiber Scaffolds as a Wound Dressing Material.

Ahire JJ, Hattingh M, Neveling DP, Dicks LM - PLoS ONE (2016)

SEM-energy-dispersive X-ray (EDX) analysis of (a) nanofibers without copper (CF), (b) copper-containing nanofibers (Cu-F) and (c) copper particles. The EDX scan spectra of selected areas pointed out in images (a), (b) and (c) are shown in (d), (e) and (f), respectively. The percentages shown for each of the elements are the mean of three selected areas from SEM images.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814046&req=5

pone.0152755.g002: SEM-energy-dispersive X-ray (EDX) analysis of (a) nanofibers without copper (CF), (b) copper-containing nanofibers (Cu-F) and (c) copper particles. The EDX scan spectra of selected areas pointed out in images (a), (b) and (c) are shown in (d), (e) and (f), respectively. The percentages shown for each of the elements are the mean of three selected areas from SEM images.
Mentions: Analyses with SEM-energy-dispersive X-ray (EDX) of Cu-F (Fig 2B) and copper particles (Fig 2C) revealed the presence of C, O, Cu, and Zn (Fig 2E and 2F). In addition, trace quantities of Al (0.43 ± 0.41 wt. %) were also detected in copper particles (Fig 2C and 2F). Analyses of CF showed C and O as major elements, with small quantities (0.45 ± 0.12 wt. %) of Si (Fig 2A and 2D).

Bottom Line: Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter).The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC).After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.

ABSTRACT
Copper particles were incorporated into nanofibers during the electrospinning of poly-D,L-lactide (PDLLA) and poly(ethylene oxide) (PEO). The ability of the nanofibers to prevent Pseudomonas aeruginosa PA01 and Staphylococcus aureus (strain Xen 30) to form biofilms was tested. Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter). The crystalline structure of the copper particles in Cu-F was confirmed by X-ray diffraction (XRD). Copper crystals were encapsulated, but also attached to the surface of Cu-F, as shown scanning transmission electron microscopy (STEM) and transmission electron microscopy (TEM), respectively. The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC). After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively. Reduction in biofilm formation was ascribed to copper released from the nanofibers. Copper-containing nanofibers may be incorporated into wound dressings.

Show MeSH
Related in: MedlinePlus