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Cordyceps sinensis protects against liver and heart injuries in a rat model of chronic kidney disease: a metabolomic analysis.

Liu X, Zhong F, Tang XL, Lian FL, Zhou Q, Guo SM, Liu JF, Sun P, Hao X, Lu Y, Wang WM, Chen N, Zhang NX - Acta Pharmacol. Sin. (2014)

Bottom Line: Within the experimental period of 8 weeks, the metabolic disorders in the liver were more pronounced than in the heart, suggesting that CKD-related extrarenal organ dysfunctions occurred sequentially rather than simultaneously.Oral administration of Cordyceps sinensis exerted statistically significant rescue effects on the liver and heart by reversely regulating levels of those metabolites that are typically perturbed in CKD.The (1)H NMR-based metabolomic approach has provided a systematic view for understanding of CKD and the drug treatment, which can also be used to elucidate the mechanisms of action of other traditional Chinese medicines.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

ABSTRACT

Aim: To test the hypothesis that the traditional Chinese medicine Cordyceps sinensis could improve the metabolic function of extrarenal organs to achieve its anti-chronic kidney disease (CKD) effects.

Methods: Male SD rats were divided into CKD rats (with 5/6-nephrectomy), CKD rats treated with Cordyceps sinensis (4 mg•kg-1•d-1, po), and sham-operated rats. After an 8-week treatment, metabolites were extracted from the hearts and livers of the rats, and then subjected to (1)H-NMR-based metabolomic analysis.

Results: Oxidative stress, energy metabolism, amino acid and protein metabolism and choline metabolism were considered as links between CKD and extrarenal organ dysfunction. Within the experimental period of 8 weeks, the metabolic disorders in the liver were more pronounced than in the heart, suggesting that CKD-related extrarenal organ dysfunctions occurred sequentially rather than simultaneously. Oral administration of Cordyceps sinensis exerted statistically significant rescue effects on the liver and heart by reversely regulating levels of those metabolites that are typically perturbed in CKD.

Conclusion: Oral administration of Cordyceps sinensis significantly attenuates the liver and heart injuries in CKD rats. The (1)H NMR-based metabolomic approach has provided a systematic view for understanding of CKD and the drug treatment, which can also be used to elucidate the mechanisms of action of other traditional Chinese medicines.

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The score plots (left) and the permutation tests (right) derived from OPLS-DA models of (A, C) CS vs OP with the 5-round cross validation, (B, D) OP vs SO with the 5-round cross validation of 1H NMR spectra of aqueous liver extracts. Variables of score plots are autoscaling all variables to unit variance.
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fig3: The score plots (left) and the permutation tests (right) derived from OPLS-DA models of (A, C) CS vs OP with the 5-round cross validation, (B, D) OP vs SO with the 5-round cross validation of 1H NMR spectra of aqueous liver extracts. Variables of score plots are autoscaling all variables to unit variance.

Mentions: The results mentioned above were supported by the following OPLS-DA analysis. The application of OPLS-DA, which used the first predictive component and one orthogonal component to optimize intergroup variation, resulted in clear biochemical distinctions between the CS and OP groups [R2X(cum)=40%, R2Y(cum)=90%, Q2(cum)=52%] (Figure 3A) and between the OP and SO groups [R2X(cum)=47%, R2Y(cum)=97%, Q2(cum)=85%] (Figure 3C). A cluster of 500 permutated models from the first component was visualized using validation plots (Figures 3B, 3D). In the permutation test plots, all of the permutated Q2 values to the left were lower than the original point to the right39,40, which indicated that the original models were valid.


Cordyceps sinensis protects against liver and heart injuries in a rat model of chronic kidney disease: a metabolomic analysis.

Liu X, Zhong F, Tang XL, Lian FL, Zhou Q, Guo SM, Liu JF, Sun P, Hao X, Lu Y, Wang WM, Chen N, Zhang NX - Acta Pharmacol. Sin. (2014)

The score plots (left) and the permutation tests (right) derived from OPLS-DA models of (A, C) CS vs OP with the 5-round cross validation, (B, D) OP vs SO with the 5-round cross validation of 1H NMR spectra of aqueous liver extracts. Variables of score plots are autoscaling all variables to unit variance.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4814030&req=5

fig3: The score plots (left) and the permutation tests (right) derived from OPLS-DA models of (A, C) CS vs OP with the 5-round cross validation, (B, D) OP vs SO with the 5-round cross validation of 1H NMR spectra of aqueous liver extracts. Variables of score plots are autoscaling all variables to unit variance.
Mentions: The results mentioned above were supported by the following OPLS-DA analysis. The application of OPLS-DA, which used the first predictive component and one orthogonal component to optimize intergroup variation, resulted in clear biochemical distinctions between the CS and OP groups [R2X(cum)=40%, R2Y(cum)=90%, Q2(cum)=52%] (Figure 3A) and between the OP and SO groups [R2X(cum)=47%, R2Y(cum)=97%, Q2(cum)=85%] (Figure 3C). A cluster of 500 permutated models from the first component was visualized using validation plots (Figures 3B, 3D). In the permutation test plots, all of the permutated Q2 values to the left were lower than the original point to the right39,40, which indicated that the original models were valid.

Bottom Line: Within the experimental period of 8 weeks, the metabolic disorders in the liver were more pronounced than in the heart, suggesting that CKD-related extrarenal organ dysfunctions occurred sequentially rather than simultaneously.Oral administration of Cordyceps sinensis exerted statistically significant rescue effects on the liver and heart by reversely regulating levels of those metabolites that are typically perturbed in CKD.The (1)H NMR-based metabolomic approach has provided a systematic view for understanding of CKD and the drug treatment, which can also be used to elucidate the mechanisms of action of other traditional Chinese medicines.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

ABSTRACT

Aim: To test the hypothesis that the traditional Chinese medicine Cordyceps sinensis could improve the metabolic function of extrarenal organs to achieve its anti-chronic kidney disease (CKD) effects.

Methods: Male SD rats were divided into CKD rats (with 5/6-nephrectomy), CKD rats treated with Cordyceps sinensis (4 mg•kg-1•d-1, po), and sham-operated rats. After an 8-week treatment, metabolites were extracted from the hearts and livers of the rats, and then subjected to (1)H-NMR-based metabolomic analysis.

Results: Oxidative stress, energy metabolism, amino acid and protein metabolism and choline metabolism were considered as links between CKD and extrarenal organ dysfunction. Within the experimental period of 8 weeks, the metabolic disorders in the liver were more pronounced than in the heart, suggesting that CKD-related extrarenal organ dysfunctions occurred sequentially rather than simultaneously. Oral administration of Cordyceps sinensis exerted statistically significant rescue effects on the liver and heart by reversely regulating levels of those metabolites that are typically perturbed in CKD.

Conclusion: Oral administration of Cordyceps sinensis significantly attenuates the liver and heart injuries in CKD rats. The (1)H NMR-based metabolomic approach has provided a systematic view for understanding of CKD and the drug treatment, which can also be used to elucidate the mechanisms of action of other traditional Chinese medicines.

Show MeSH
Related in: MedlinePlus