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Cordyceps sinensis protects against liver and heart injuries in a rat model of chronic kidney disease: a metabolomic analysis.

Liu X, Zhong F, Tang XL, Lian FL, Zhou Q, Guo SM, Liu JF, Sun P, Hao X, Lu Y, Wang WM, Chen N, Zhang NX - Acta Pharmacol. Sin. (2014)

Bottom Line: Within the experimental period of 8 weeks, the metabolic disorders in the liver were more pronounced than in the heart, suggesting that CKD-related extrarenal organ dysfunctions occurred sequentially rather than simultaneously.Oral administration of Cordyceps sinensis exerted statistically significant rescue effects on the liver and heart by reversely regulating levels of those metabolites that are typically perturbed in CKD.The (1)H NMR-based metabolomic approach has provided a systematic view for understanding of CKD and the drug treatment, which can also be used to elucidate the mechanisms of action of other traditional Chinese medicines.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

ABSTRACT

Aim: To test the hypothesis that the traditional Chinese medicine Cordyceps sinensis could improve the metabolic function of extrarenal organs to achieve its anti-chronic kidney disease (CKD) effects.

Methods: Male SD rats were divided into CKD rats (with 5/6-nephrectomy), CKD rats treated with Cordyceps sinensis (4 mg•kg-1•d-1, po), and sham-operated rats. After an 8-week treatment, metabolites were extracted from the hearts and livers of the rats, and then subjected to (1)H-NMR-based metabolomic analysis.

Results: Oxidative stress, energy metabolism, amino acid and protein metabolism and choline metabolism were considered as links between CKD and extrarenal organ dysfunction. Within the experimental period of 8 weeks, the metabolic disorders in the liver were more pronounced than in the heart, suggesting that CKD-related extrarenal organ dysfunctions occurred sequentially rather than simultaneously. Oral administration of Cordyceps sinensis exerted statistically significant rescue effects on the liver and heart by reversely regulating levels of those metabolites that are typically perturbed in CKD.

Conclusion: Oral administration of Cordyceps sinensis significantly attenuates the liver and heart injuries in CKD rats. The (1)H NMR-based metabolomic approach has provided a systematic view for understanding of CKD and the drug treatment, which can also be used to elucidate the mechanisms of action of other traditional Chinese medicines.

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PLS-DA score plots of 1H NMR analysis of aqueous liver extracts (A) and aqueous heart extracts (B). Model parameters are as follows: (A) R2X(cum)=0.37, R2Y(cum)=0.82, Q2(cum)=0.57; (B) R2X(cum)=0.52, R2Y(cum)=0.60, Q2(cum)=0.22.
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fig2: PLS-DA score plots of 1H NMR analysis of aqueous liver extracts (A) and aqueous heart extracts (B). Model parameters are as follows: (A) R2X(cum)=0.37, R2Y(cum)=0.82, Q2(cum)=0.57; (B) R2X(cum)=0.52, R2Y(cum)=0.60, Q2(cum)=0.22.

Mentions: The PCA score plots, which were obtained from the multivariate statistical analysis of the unambiguously identified 39 variables from the liver aqueous extracts of the three groups, revealed a group clustering for the metabolic profiles of the OP group versus the SO group samples (Figure S3A). Consistently, a distinct separation between the OP group and the SO group was also present in the PLS-DA score plot along the PC1 axis, and, in comparison with the OP group, the CS group cluster moved closer to that of the SO group, indicating that C sinensis administration ameliorated the metabolic disorders in the liver tissue of CKD rats (Figure 2A).


Cordyceps sinensis protects against liver and heart injuries in a rat model of chronic kidney disease: a metabolomic analysis.

Liu X, Zhong F, Tang XL, Lian FL, Zhou Q, Guo SM, Liu JF, Sun P, Hao X, Lu Y, Wang WM, Chen N, Zhang NX - Acta Pharmacol. Sin. (2014)

PLS-DA score plots of 1H NMR analysis of aqueous liver extracts (A) and aqueous heart extracts (B). Model parameters are as follows: (A) R2X(cum)=0.37, R2Y(cum)=0.82, Q2(cum)=0.57; (B) R2X(cum)=0.52, R2Y(cum)=0.60, Q2(cum)=0.22.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4814030&req=5

fig2: PLS-DA score plots of 1H NMR analysis of aqueous liver extracts (A) and aqueous heart extracts (B). Model parameters are as follows: (A) R2X(cum)=0.37, R2Y(cum)=0.82, Q2(cum)=0.57; (B) R2X(cum)=0.52, R2Y(cum)=0.60, Q2(cum)=0.22.
Mentions: The PCA score plots, which were obtained from the multivariate statistical analysis of the unambiguously identified 39 variables from the liver aqueous extracts of the three groups, revealed a group clustering for the metabolic profiles of the OP group versus the SO group samples (Figure S3A). Consistently, a distinct separation between the OP group and the SO group was also present in the PLS-DA score plot along the PC1 axis, and, in comparison with the OP group, the CS group cluster moved closer to that of the SO group, indicating that C sinensis administration ameliorated the metabolic disorders in the liver tissue of CKD rats (Figure 2A).

Bottom Line: Within the experimental period of 8 weeks, the metabolic disorders in the liver were more pronounced than in the heart, suggesting that CKD-related extrarenal organ dysfunctions occurred sequentially rather than simultaneously.Oral administration of Cordyceps sinensis exerted statistically significant rescue effects on the liver and heart by reversely regulating levels of those metabolites that are typically perturbed in CKD.The (1)H NMR-based metabolomic approach has provided a systematic view for understanding of CKD and the drug treatment, which can also be used to elucidate the mechanisms of action of other traditional Chinese medicines.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

ABSTRACT

Aim: To test the hypothesis that the traditional Chinese medicine Cordyceps sinensis could improve the metabolic function of extrarenal organs to achieve its anti-chronic kidney disease (CKD) effects.

Methods: Male SD rats were divided into CKD rats (with 5/6-nephrectomy), CKD rats treated with Cordyceps sinensis (4 mg•kg-1•d-1, po), and sham-operated rats. After an 8-week treatment, metabolites were extracted from the hearts and livers of the rats, and then subjected to (1)H-NMR-based metabolomic analysis.

Results: Oxidative stress, energy metabolism, amino acid and protein metabolism and choline metabolism were considered as links between CKD and extrarenal organ dysfunction. Within the experimental period of 8 weeks, the metabolic disorders in the liver were more pronounced than in the heart, suggesting that CKD-related extrarenal organ dysfunctions occurred sequentially rather than simultaneously. Oral administration of Cordyceps sinensis exerted statistically significant rescue effects on the liver and heart by reversely regulating levels of those metabolites that are typically perturbed in CKD.

Conclusion: Oral administration of Cordyceps sinensis significantly attenuates the liver and heart injuries in CKD rats. The (1)H NMR-based metabolomic approach has provided a systematic view for understanding of CKD and the drug treatment, which can also be used to elucidate the mechanisms of action of other traditional Chinese medicines.

Show MeSH
Related in: MedlinePlus