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Human umbilical cord blood mesenchymal stem cell transplantation suppresses inflammatory responses and neuronal apoptosis during early stage of focal cerebral ischemia in rabbits.

Zhu Y, Guan YM, Huang HL, Wang QS - Acta Pharmacol. Sin. (2014)

Bottom Line: Inflammatory cell infiltration, neuronal apoptosis and neuronal density were measured morphologically. hUCB-MSCs (5 × 10(6)) were iv injected a few minutes after MCAO.Focal cerebral ischemia decreased the serum level of IL-10, which was prevented by hUCB-MSC transplantation.Furthermore, hUCB-MSC transplantation significantly decreased the percentage of apoptosis around the ischemic region. hUCB-MSCs transplantation suppresses inflammatory responses and neuronal apoptosis during the early stage focal cerebral ischemia in rabbits.

View Article: PubMed Central - PubMed

Affiliation: 1] Departments of Neurology, The 105th Hospital of PLA, Hefei 230031, China [2] Clinic College, Anhui Medical University, Hefei 230032, China.

ABSTRACT

Aim: Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) have been shown to ameliorate cerebral ischemia in animal models. In this study we investigated the effects of hUCB-MSCs on inflammatory responses and neuronal apoptosis during the early stage of focal cerebral ischemia in rabbits.

Methods: Focal cerebral ischemia was induced in male New Zealand rabbits by occlusion of MCA for 2 h. The blood samples were collected at different time points prior and during MCAO-reperfusion. The animals were euthanized 3 d after MCAO, and the protein levels of IL-1β, IL-6, IL-10 and TNF-α in the serum and peri-ischemic brain tissues were detected using Western blot and ELISA, respectively. Inflammatory cell infiltration, neuronal apoptosis and neuronal density were measured morphologically. hUCB-MSCs (5 × 10(6)) were iv injected a few minutes after MCAO.

Results: The serum levels of IL-1β, IL-6 and TNF-α were rapidly increased, and peaked at 2 h after the start of MCAO. hUCB-MSC transplantation markedly and progressively suppressed the ischemia-induced increases of serum IL-1β, IL-6 and TNF-α levels within 6 h MCAO-reperfusion. Focal cerebral ischemia decreased the serum level of IL-10, which was prevented by hUCB-MSC transplantation. The expression of IL-1β, IL-6, IL-10 and TNF-α in the peri-ischemic brain tissues showed similar changes as in the serum. hUCB-MSC transplantation markedly suppressed the infiltration of inflammatory cells, and increased the neuronal density around the ischemic region. Furthermore, hUCB-MSC transplantation significantly decreased the percentage of apoptosis around the ischemic region.

Conclusion: hUCB-MSCs transplantation suppresses inflammatory responses and neuronal apoptosis during the early stage focal cerebral ischemia in rabbits.

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Related in: MedlinePlus

Evaluation of IL-1β, IL-6, IL-10, and TNF-α protein production in peri-ischemic region by Western blot. bP<0.05 vs SH. eP<0.05 vs IR or IR+NS.
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fig2: Evaluation of IL-1β, IL-6, IL-10, and TNF-α protein production in peri-ischemic region by Western blot. bP<0.05 vs SH. eP<0.05 vs IR or IR+NS.

Mentions: Western blot analysis was performed for each inflammatory factor of interest. Compared with the IR and IR+NS groups, the IR+hUCB-MSC group had a decreased protein expression of IL-1β, IL-6, and TNF-α and a markedly increased protein expression of IL-10 (P<0.05) (Figure 2).


Human umbilical cord blood mesenchymal stem cell transplantation suppresses inflammatory responses and neuronal apoptosis during early stage of focal cerebral ischemia in rabbits.

Zhu Y, Guan YM, Huang HL, Wang QS - Acta Pharmacol. Sin. (2014)

Evaluation of IL-1β, IL-6, IL-10, and TNF-α protein production in peri-ischemic region by Western blot. bP<0.05 vs SH. eP<0.05 vs IR or IR+NS.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4814029&req=5

fig2: Evaluation of IL-1β, IL-6, IL-10, and TNF-α protein production in peri-ischemic region by Western blot. bP<0.05 vs SH. eP<0.05 vs IR or IR+NS.
Mentions: Western blot analysis was performed for each inflammatory factor of interest. Compared with the IR and IR+NS groups, the IR+hUCB-MSC group had a decreased protein expression of IL-1β, IL-6, and TNF-α and a markedly increased protein expression of IL-10 (P<0.05) (Figure 2).

Bottom Line: Inflammatory cell infiltration, neuronal apoptosis and neuronal density were measured morphologically. hUCB-MSCs (5 × 10(6)) were iv injected a few minutes after MCAO.Focal cerebral ischemia decreased the serum level of IL-10, which was prevented by hUCB-MSC transplantation.Furthermore, hUCB-MSC transplantation significantly decreased the percentage of apoptosis around the ischemic region. hUCB-MSCs transplantation suppresses inflammatory responses and neuronal apoptosis during the early stage focal cerebral ischemia in rabbits.

View Article: PubMed Central - PubMed

Affiliation: 1] Departments of Neurology, The 105th Hospital of PLA, Hefei 230031, China [2] Clinic College, Anhui Medical University, Hefei 230032, China.

ABSTRACT

Aim: Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) have been shown to ameliorate cerebral ischemia in animal models. In this study we investigated the effects of hUCB-MSCs on inflammatory responses and neuronal apoptosis during the early stage of focal cerebral ischemia in rabbits.

Methods: Focal cerebral ischemia was induced in male New Zealand rabbits by occlusion of MCA for 2 h. The blood samples were collected at different time points prior and during MCAO-reperfusion. The animals were euthanized 3 d after MCAO, and the protein levels of IL-1β, IL-6, IL-10 and TNF-α in the serum and peri-ischemic brain tissues were detected using Western blot and ELISA, respectively. Inflammatory cell infiltration, neuronal apoptosis and neuronal density were measured morphologically. hUCB-MSCs (5 × 10(6)) were iv injected a few minutes after MCAO.

Results: The serum levels of IL-1β, IL-6 and TNF-α were rapidly increased, and peaked at 2 h after the start of MCAO. hUCB-MSC transplantation markedly and progressively suppressed the ischemia-induced increases of serum IL-1β, IL-6 and TNF-α levels within 6 h MCAO-reperfusion. Focal cerebral ischemia decreased the serum level of IL-10, which was prevented by hUCB-MSC transplantation. The expression of IL-1β, IL-6, IL-10 and TNF-α in the peri-ischemic brain tissues showed similar changes as in the serum. hUCB-MSC transplantation markedly suppressed the infiltration of inflammatory cells, and increased the neuronal density around the ischemic region. Furthermore, hUCB-MSC transplantation significantly decreased the percentage of apoptosis around the ischemic region.

Conclusion: hUCB-MSCs transplantation suppresses inflammatory responses and neuronal apoptosis during the early stage focal cerebral ischemia in rabbits.

Show MeSH
Related in: MedlinePlus