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Intranasal co-delivery of IL-6 gene enhances the immunogenicity of anti-caries DNA vaccine.

Su LK, Yu F, Li ZF, Zeng C, Xu QA, Fan MW - Acta Pharmacol. Sin. (2014)

Bottom Line: To investigate the effects of co-delivering IL-6 expressing plasmid pCI-IL-6 on the immunogenicity of the anti-caries DNA vaccine pCIA-P, which encodes the surface protein antigen PAc of Streptococcus mutans.Marked expression of IL-6 was found in COS-7 cells transfected with pCI-IL-6.Intranasal co-delivery of IL-6 gene significantly enhances the immunogenicity of the anti-caries DNA vaccine.

View Article: PubMed Central - PubMed

Affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine of Ministry of Education (KLOBM), School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.

ABSTRACT

Aim: To investigate the effects of co-delivering IL-6 expressing plasmid pCI-IL-6 on the immunogenicity of the anti-caries DNA vaccine pCIA-P, which encodes the surface protein antigen PAc of Streptococcus mutans.

Methods: Plasmid pCI-IL-6 was constructed by inserting the murine IL-6 gene into the pCI vector. Expression of IL-6 in vitro was assessed using Western blot analysis. BALB/c mice were intranasally co-immunized with pCIA-P plus pCI-IL-6 on d 0 and 14. Anti-PAc IgG and secretory IgA (sIgA) were assessed by ELISA. Splenocytes from the mice were re-stimulated with the PAc protein, and IFN-γ and IL-4 production was measured using ELISA. Splenocyte proliferation was analyzed with flow cytometry. Rats were similarly immunized, and dental caries scores were determined using the Keyes method.

Results: Marked expression of IL-6 was found in COS-7 cells transfected with pCI-IL-6. In the pCI-IL-6 co-immunized mice, the specific IgG antibodies in serum and sIgA antibodies in saliva were significantly higher than those in the control mice at weeks 4 and 8. Moreover, the secretion of IFN-γ from splenocytes in response to re-stimulation with PAc protein was significantly higher in the pCI-IL-6 co-immunized mice than that in the control mice, whereas the secretion of IL-4 had no significant difference. The proliferation of splenocytes from the pCI-IL-6 co-immunized mice was significantly higher than that from the mice immunized with pCIA-P and pCI vector. In the rat caries model, the pCI-IL-6 co-immunization rats displayed lower caries scores than the control rats.

Conclusion: Intranasal co-delivery of IL-6 gene significantly enhances the immunogenicity of the anti-caries DNA vaccine.

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Related in: MedlinePlus

Levels of saliva specific IgA antibody. Saliva samples were collected from mice immunized intranasally with pCIA-P plus pCI-IL-6 or pCIA-P plus pCI vector every 4 weeks for ELISA. The results are shown as the ratios of specific anti-PAc salivary IgA to total IgA. Mean±SEM. n=8. cP<0.01 vs pCIA-P+pCI group.
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fig4: Levels of saliva specific IgA antibody. Saliva samples were collected from mice immunized intranasally with pCIA-P plus pCI-IL-6 or pCIA-P plus pCI vector every 4 weeks for ELISA. The results are shown as the ratios of specific anti-PAc salivary IgA to total IgA. Mean±SEM. n=8. cP<0.01 vs pCIA-P+pCI group.

Mentions: In this study, the ratio of specific anti-PAc salivary IgA to total IgA was used to represent the intensity of the salivary IgA responses. The saliva specific IgA antibody levels in the pCIA-P plus pCI-IL-6 immunization group were significantly higher than that in the pCIA-P plus pCI immunization group at weeks 4 and 8 (P<0.01) (Figure 4). However, there was no statistically significant difference (P>0.05) between the two groups at weeks 12 and 16. The highest level of saliva specific IgA antibody was also at week 8.


Intranasal co-delivery of IL-6 gene enhances the immunogenicity of anti-caries DNA vaccine.

Su LK, Yu F, Li ZF, Zeng C, Xu QA, Fan MW - Acta Pharmacol. Sin. (2014)

Levels of saliva specific IgA antibody. Saliva samples were collected from mice immunized intranasally with pCIA-P plus pCI-IL-6 or pCIA-P plus pCI vector every 4 weeks for ELISA. The results are shown as the ratios of specific anti-PAc salivary IgA to total IgA. Mean±SEM. n=8. cP<0.01 vs pCIA-P+pCI group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4814028&req=5

fig4: Levels of saliva specific IgA antibody. Saliva samples were collected from mice immunized intranasally with pCIA-P plus pCI-IL-6 or pCIA-P plus pCI vector every 4 weeks for ELISA. The results are shown as the ratios of specific anti-PAc salivary IgA to total IgA. Mean±SEM. n=8. cP<0.01 vs pCIA-P+pCI group.
Mentions: In this study, the ratio of specific anti-PAc salivary IgA to total IgA was used to represent the intensity of the salivary IgA responses. The saliva specific IgA antibody levels in the pCIA-P plus pCI-IL-6 immunization group were significantly higher than that in the pCIA-P plus pCI immunization group at weeks 4 and 8 (P<0.01) (Figure 4). However, there was no statistically significant difference (P>0.05) between the two groups at weeks 12 and 16. The highest level of saliva specific IgA antibody was also at week 8.

Bottom Line: To investigate the effects of co-delivering IL-6 expressing plasmid pCI-IL-6 on the immunogenicity of the anti-caries DNA vaccine pCIA-P, which encodes the surface protein antigen PAc of Streptococcus mutans.Marked expression of IL-6 was found in COS-7 cells transfected with pCI-IL-6.Intranasal co-delivery of IL-6 gene significantly enhances the immunogenicity of the anti-caries DNA vaccine.

View Article: PubMed Central - PubMed

Affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine of Ministry of Education (KLOBM), School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.

ABSTRACT

Aim: To investigate the effects of co-delivering IL-6 expressing plasmid pCI-IL-6 on the immunogenicity of the anti-caries DNA vaccine pCIA-P, which encodes the surface protein antigen PAc of Streptococcus mutans.

Methods: Plasmid pCI-IL-6 was constructed by inserting the murine IL-6 gene into the pCI vector. Expression of IL-6 in vitro was assessed using Western blot analysis. BALB/c mice were intranasally co-immunized with pCIA-P plus pCI-IL-6 on d 0 and 14. Anti-PAc IgG and secretory IgA (sIgA) were assessed by ELISA. Splenocytes from the mice were re-stimulated with the PAc protein, and IFN-γ and IL-4 production was measured using ELISA. Splenocyte proliferation was analyzed with flow cytometry. Rats were similarly immunized, and dental caries scores were determined using the Keyes method.

Results: Marked expression of IL-6 was found in COS-7 cells transfected with pCI-IL-6. In the pCI-IL-6 co-immunized mice, the specific IgG antibodies in serum and sIgA antibodies in saliva were significantly higher than those in the control mice at weeks 4 and 8. Moreover, the secretion of IFN-γ from splenocytes in response to re-stimulation with PAc protein was significantly higher in the pCI-IL-6 co-immunized mice than that in the control mice, whereas the secretion of IL-4 had no significant difference. The proliferation of splenocytes from the pCI-IL-6 co-immunized mice was significantly higher than that from the mice immunized with pCIA-P and pCI vector. In the rat caries model, the pCI-IL-6 co-immunization rats displayed lower caries scores than the control rats.

Conclusion: Intranasal co-delivery of IL-6 gene significantly enhances the immunogenicity of the anti-caries DNA vaccine.

Show MeSH
Related in: MedlinePlus