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Influence of tebuconazole and copper hydroxide on phosphatase and urease activities in red sandy loam and black clay soils

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ABSTRACT

The efficacy of two selected fungicides i.e., tebuconazole and coppoer hydroxide, was conducted experiments in laboratory and copper hydroxide on the two specific enzymes phosphatase and urease were determined in two different soil samples (red sandy loam and black clay soils) of groundnut (Arachis hypogaea L.) from cultivated fields of Anantapuramu District, Andhra Pradesh. The activities of the selected soil enzymes were determined by incubating the selected fungicides-treated (1.0, 2.5, 5.0, 7.5 and 10.0 kg ha−1) and -untreated groundnut soil samples at 10 day intervals. By determining the effective concentration, the rate of selected enzyme activity was estimated by adding the suitable substrate at 10, 20, 30 and 40 days of soil incubation. Both the enzyme activities were increased up to 5.0 kg ha−1 level of fungicide in both soil samples significantly at 10 days of soil incubation and further enhanced up to 20 days of incubation. The activity of the phosphatase and urease decreased progressively at 30 and 40 days of incubation. From overall studies, higher concentrations (7.5 and 10.0 kg ha−1) of both tebuconazole and copper hydroxide were toxic to phosphatase and urease activities, respectively, in both soil samples.

No MeSH data available.


a, b Influence of tebuconazole and copper hydroxide on phosphatasea activity in black clay and red sandy loam soil at 5.0 kg ha−1. Asterisk µg of p-nitrophenol (PNP) g−1 soil formed after 24 h of incubation with p-nitrophenyl phosphate (PNPP), after 10, 20, 30 and 40 days of incubation. The values are the mean ± SE for each incubation period and are not significantly different (P ≤ 0.05) from each other according to Duncan’s multiple range (DMR) test
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Fig1: a, b Influence of tebuconazole and copper hydroxide on phosphatasea activity in black clay and red sandy loam soil at 5.0 kg ha−1. Asterisk µg of p-nitrophenol (PNP) g−1 soil formed after 24 h of incubation with p-nitrophenyl phosphate (PNPP), after 10, 20, 30 and 40 days of incubation. The values are the mean ± SE for each incubation period and are not significantly different (P ≤ 0.05) from each other according to Duncan’s multiple range (DMR) test

Mentions: From the results obtained from the present study, about 32–221 and 42–210 % increase in phosphatase activity over the control was noticed in the black soil with 20 days of incubation, whereas in the case of red sandy loam soil, the corresponding figures of the percentage enhancement by the two selected fungicides at two levels were 63–368 and 83–342 % during the same period of incubation. In comparison, tebuconazole at 5.0 kg ha−1 produced maximum stimulation in phosphatase activity in black clay soil than red sandy loam soil. At higher concentrations, i.e., 7.5 and 10.0 kg ha−1, phosphatase activity was significantly inhibited by treating the selected soil samples with both the selected fungicides. Among the two fungicide treatments, tebuconazole produced a different stimulation over the control. In the present study, comparatively, black soil showed higher enzyme activity than red soil throughout the experiment. Phosphatase activity was significantly inhibited at higher concentrations, i.e., 7.5 and 10.0 kg ha−1 in both the fungicide treatments, gradually with the incubation periods, i.e., 20, 30 and 40 days (Rangaswamy and Venkateswarlu 1996). Therefore in the present experiment, the maximum inhibition was recorded after 30 and 40 days of incubation periods. The enhancement in phosphatase activity over control was noticed in the black soil after 20 days of incubation. In the case of red sandy loam soil, the enhancement by the selected fungicides was also obtained at 5.0 kg ha−1 for 20 days of incubation and recorded in Table 2. The inhibitory effect was reduced upon further incubation, i.e., 30 and 40 days, due to the reduction in the concentration and degradation of the applied fungicides (Fig. 1).Fig. 1


Influence of tebuconazole and copper hydroxide on phosphatase and urease activities in red sandy loam and black clay soils
a, b Influence of tebuconazole and copper hydroxide on phosphatasea activity in black clay and red sandy loam soil at 5.0 kg ha−1. Asterisk µg of p-nitrophenol (PNP) g−1 soil formed after 24 h of incubation with p-nitrophenyl phosphate (PNPP), after 10, 20, 30 and 40 days of incubation. The values are the mean ± SE for each incubation period and are not significantly different (P ≤ 0.05) from each other according to Duncan’s multiple range (DMR) test
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Fig1: a, b Influence of tebuconazole and copper hydroxide on phosphatasea activity in black clay and red sandy loam soil at 5.0 kg ha−1. Asterisk µg of p-nitrophenol (PNP) g−1 soil formed after 24 h of incubation with p-nitrophenyl phosphate (PNPP), after 10, 20, 30 and 40 days of incubation. The values are the mean ± SE for each incubation period and are not significantly different (P ≤ 0.05) from each other according to Duncan’s multiple range (DMR) test
Mentions: From the results obtained from the present study, about 32–221 and 42–210 % increase in phosphatase activity over the control was noticed in the black soil with 20 days of incubation, whereas in the case of red sandy loam soil, the corresponding figures of the percentage enhancement by the two selected fungicides at two levels were 63–368 and 83–342 % during the same period of incubation. In comparison, tebuconazole at 5.0 kg ha−1 produced maximum stimulation in phosphatase activity in black clay soil than red sandy loam soil. At higher concentrations, i.e., 7.5 and 10.0 kg ha−1, phosphatase activity was significantly inhibited by treating the selected soil samples with both the selected fungicides. Among the two fungicide treatments, tebuconazole produced a different stimulation over the control. In the present study, comparatively, black soil showed higher enzyme activity than red soil throughout the experiment. Phosphatase activity was significantly inhibited at higher concentrations, i.e., 7.5 and 10.0 kg ha−1 in both the fungicide treatments, gradually with the incubation periods, i.e., 20, 30 and 40 days (Rangaswamy and Venkateswarlu 1996). Therefore in the present experiment, the maximum inhibition was recorded after 30 and 40 days of incubation periods. The enhancement in phosphatase activity over control was noticed in the black soil after 20 days of incubation. In the case of red sandy loam soil, the enhancement by the selected fungicides was also obtained at 5.0 kg ha−1 for 20 days of incubation and recorded in Table 2. The inhibitory effect was reduced upon further incubation, i.e., 30 and 40 days, due to the reduction in the concentration and degradation of the applied fungicides (Fig. 1).Fig. 1

View Article: PubMed Central - PubMed

ABSTRACT

The efficacy of two selected fungicides i.e., tebuconazole and coppoer hydroxide, was conducted experiments in laboratory and copper hydroxide on the two specific enzymes phosphatase and urease were determined in two different soil samples (red sandy loam and black clay soils) of groundnut (Arachis hypogaea L.) from cultivated fields of Anantapuramu District, Andhra Pradesh. The activities of the selected soil enzymes were determined by incubating the selected fungicides-treated (1.0, 2.5, 5.0, 7.5 and 10.0 kg ha−1) and -untreated groundnut soil samples at 10 day intervals. By determining the effective concentration, the rate of selected enzyme activity was estimated by adding the suitable substrate at 10, 20, 30 and 40 days of soil incubation. Both the enzyme activities were increased up to 5.0 kg ha−1 level of fungicide in both soil samples significantly at 10 days of soil incubation and further enhanced up to 20 days of incubation. The activity of the phosphatase and urease decreased progressively at 30 and 40 days of incubation. From overall studies, higher concentrations (7.5 and 10.0 kg ha−1) of both tebuconazole and copper hydroxide were toxic to phosphatase and urease activities, respectively, in both soil samples.

No MeSH data available.