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MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars

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ABSTRACT

A MYB transcription factor gene, RsMYB1, from radish was introduced into the chrysanthemum cultivars ‘Peach ND’, ‘Peach Red’, and ‘Vivid Scarlet’ under the control of the cauliflower mosaic virus 35S promoter. Presence of RsMYB1 in transgenic lines was confirmed using polymerase chain reaction (PCR). Results of reverse-transcription-PCR analysis revealed that the expression of RsMYB1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (F3H, DFR, and ANS) involved in anthocyanin production. Accordingly, anthocyanin content was significantly higher in transgenic lines than in control of all the cultivars, although the increasement was not visually observed in any of the transgenic lines. Therefore, these results demonstrate that RsMYB1 has potential to enhance anthocyanin content in the chrysanthemums.

No MeSH data available.


RT-PCR analysis of RsMYB1 expression in the non-transgenic lines (wild type) and the transgenic lines of the chrysanthemum cultivars, namely, Peach ND (PN), Peach Red (PR), and Vivid Scarlet (VD)
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Fig2: RT-PCR analysis of RsMYB1 expression in the non-transgenic lines (wild type) and the transgenic lines of the chrysanthemum cultivars, namely, Peach ND (PN), Peach Red (PR), and Vivid Scarlet (VD)

Mentions: We used the two independent transgenic lines derived from each cultivar to perform an expression analysis of RsMYB1 and anthocyanin biosynthetic genes. Results of RT-PCR showed that distinct and stable expression of RsMYB1 in all individual lines of the same genotype (Fig. 2), but its expression level was different along with different genotype tested. Surprisingly, distinct and stable expression of RsMYB1 in the cultivars did not enhance anthocyanin production.Fig. 2


MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars
RT-PCR analysis of RsMYB1 expression in the non-transgenic lines (wild type) and the transgenic lines of the chrysanthemum cultivars, namely, Peach ND (PN), Peach Red (PR), and Vivid Scarlet (VD)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4764610&req=5

Fig2: RT-PCR analysis of RsMYB1 expression in the non-transgenic lines (wild type) and the transgenic lines of the chrysanthemum cultivars, namely, Peach ND (PN), Peach Red (PR), and Vivid Scarlet (VD)
Mentions: We used the two independent transgenic lines derived from each cultivar to perform an expression analysis of RsMYB1 and anthocyanin biosynthetic genes. Results of RT-PCR showed that distinct and stable expression of RsMYB1 in all individual lines of the same genotype (Fig. 2), but its expression level was different along with different genotype tested. Surprisingly, distinct and stable expression of RsMYB1 in the cultivars did not enhance anthocyanin production.Fig. 2

View Article: PubMed Central - PubMed

ABSTRACT

A MYB transcription factor gene, RsMYB1, from radish was introduced into the chrysanthemum cultivars ‘Peach ND’, ‘Peach Red’, and ‘Vivid Scarlet’ under the control of the cauliflower mosaic virus 35S promoter. Presence of RsMYB1 in transgenic lines was confirmed using polymerase chain reaction (PCR). Results of reverse-transcription-PCR analysis revealed that the expression of RsMYB1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (F3H, DFR, and ANS) involved in anthocyanin production. Accordingly, anthocyanin content was significantly higher in transgenic lines than in control of all the cultivars, although the increasement was not visually observed in any of the transgenic lines. Therefore, these results demonstrate that RsMYB1 has potential to enhance anthocyanin content in the chrysanthemums.

No MeSH data available.