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MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars

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ABSTRACT

A MYB transcription factor gene, RsMYB1, from radish was introduced into the chrysanthemum cultivars ‘Peach ND’, ‘Peach Red’, and ‘Vivid Scarlet’ under the control of the cauliflower mosaic virus 35S promoter. Presence of RsMYB1 in transgenic lines was confirmed using polymerase chain reaction (PCR). Results of reverse-transcription-PCR analysis revealed that the expression of RsMYB1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (F3H, DFR, and ANS) involved in anthocyanin production. Accordingly, anthocyanin content was significantly higher in transgenic lines than in control of all the cultivars, although the increasement was not visually observed in any of the transgenic lines. Therefore, these results demonstrate that RsMYB1 has potential to enhance anthocyanin content in the chrysanthemums.

No MeSH data available.


PCR detection of selection marker (bar), target (RsMYB1) genes in transgenic chrysanthemum (cvs. Peach ND (PN), Peach Red (PR), and Vivid Scarlet (VD) lines1 and 2
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Fig1: PCR detection of selection marker (bar), target (RsMYB1) genes in transgenic chrysanthemum (cvs. Peach ND (PN), Peach Red (PR), and Vivid Scarlet (VD) lines1 and 2

Mentions: To investigate whether RsMYB1 could enhance the expression level of biosynthetic genes responsible for anthocyanin biosynthesis, we produced two independent transgenic lines from the cultivars ‘Peach ND’, ‘Peach Red’, and ‘Vivid Scarlet’, in which the presence of the selection marker and transgene were detected using PCR (Fig. 1). Interestingly, enhanced anthocyanin expression was not observed in the transgenic plants of the cultivars. Thus, it was necessary to investigate the expression levels of the transgene and anthocyanin biosynthetic genes.Fig. 1


MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars
PCR detection of selection marker (bar), target (RsMYB1) genes in transgenic chrysanthemum (cvs. Peach ND (PN), Peach Red (PR), and Vivid Scarlet (VD) lines1 and 2
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4764610&req=5

Fig1: PCR detection of selection marker (bar), target (RsMYB1) genes in transgenic chrysanthemum (cvs. Peach ND (PN), Peach Red (PR), and Vivid Scarlet (VD) lines1 and 2
Mentions: To investigate whether RsMYB1 could enhance the expression level of biosynthetic genes responsible for anthocyanin biosynthesis, we produced two independent transgenic lines from the cultivars ‘Peach ND’, ‘Peach Red’, and ‘Vivid Scarlet’, in which the presence of the selection marker and transgene were detected using PCR (Fig. 1). Interestingly, enhanced anthocyanin expression was not observed in the transgenic plants of the cultivars. Thus, it was necessary to investigate the expression levels of the transgene and anthocyanin biosynthetic genes.Fig. 1

View Article: PubMed Central - PubMed

ABSTRACT

A MYB transcription factor gene, RsMYB1, from radish was introduced into the chrysanthemum cultivars ‘Peach ND’, ‘Peach Red’, and ‘Vivid Scarlet’ under the control of the cauliflower mosaic virus 35S promoter. Presence of RsMYB1 in transgenic lines was confirmed using polymerase chain reaction (PCR). Results of reverse-transcription-PCR analysis revealed that the expression of RsMYB1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (F3H, DFR, and ANS) involved in anthocyanin production. Accordingly, anthocyanin content was significantly higher in transgenic lines than in control of all the cultivars, although the increasement was not visually observed in any of the transgenic lines. Therefore, these results demonstrate that RsMYB1 has potential to enhance anthocyanin content in the chrysanthemums.

No MeSH data available.