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A unique lineage gives rise to the meibomian gland.

Call M, Fischesser K, Lunn MO, Kao WW - Mol. Vis. (2016)

Bottom Line: This colocalization region decreased as the gland developed becoming restricted to the central duct near the opening to the acini in the fully developed gland.This unique group of cells was located in the central duct with a concentration near the ductule orifice.The expression of these cells reduced during meibomian gland morphogenesis and may play a role in the development and homeostasis of the gland.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Cincinnati, Cincinnati OH.

ABSTRACT

Purpose: To identify the lineage that contributes to the morphogenesis of the meibomian gland.

Methods: To examine which cell lineage gives rise to the meibomian gland, the expression of Pax6 as well as that of various cytokeratin markers, including keratin 14 (Krt14), Krt15, Krt4, and Krt10, was examined with immunofluorescent staining of C57BL/6J mouse eyelids from P2 to P11 pups and adult mice.

Results: Pax6 was localized to the cytoplasm within the acinar region of the meibomian glands during morphogenesis but was absent in the fully developed gland. Keratin 14 was expressed throughout the gland at all stages whereas keratin 15 was absent at all stages. Keratin 4, a marker of mucosal lineage, was present throughout the gland and was colocalized with keratin 10 (epidermal lineage marker) in the developing duct at P4. This colocalization region decreased as the gland developed becoming restricted to the central duct near the opening to the acini in the fully developed gland.

Conclusions: We identified a unique cell lineage that expresses markers characteristic of mucosal and epidermal epithelia during meibomian gland morphogenesis. This unique group of cells was located in the central duct with a concentration near the ductule orifice. The expression of these cells reduced during meibomian gland morphogenesis and may play a role in the development and homeostasis of the gland.

No MeSH data available.


Related in: MedlinePlus

Differential expression of Pax6. Ten-micron horizontal cryosections of the eyelid were examined for Pax6 expression. The developing meibomian gland expressed cytoplasmic Pax6 as early as P4 in the elongating epithelial cord. At P7 and P11, expression was observed within the acinar region of the gland. Once the gland was fully developed (adult), Pax6 expression was significantly reduced within the acini. MG = meibomian gland; HF = hair follicle; EP = epidermis.
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f2: Differential expression of Pax6. Ten-micron horizontal cryosections of the eyelid were examined for Pax6 expression. The developing meibomian gland expressed cytoplasmic Pax6 as early as P4 in the elongating epithelial cord. At P7 and P11, expression was observed within the acinar region of the gland. Once the gland was fully developed (adult), Pax6 expression was significantly reduced within the acini. MG = meibomian gland; HF = hair follicle; EP = epidermis.

Mentions: Pax6 is a transcription factor expressed by ectodermal tissue and plays a critical role in the development and morphogenesis of many ocular tissues. Cytoplasmic Pax6 expression was present in the acinar cells throughout all stages of meibomian gland morphogenesis, but this expression was significantly reduced in the adult meibomian glands (Figure 2).


A unique lineage gives rise to the meibomian gland.

Call M, Fischesser K, Lunn MO, Kao WW - Mol. Vis. (2016)

Differential expression of Pax6. Ten-micron horizontal cryosections of the eyelid were examined for Pax6 expression. The developing meibomian gland expressed cytoplasmic Pax6 as early as P4 in the elongating epithelial cord. At P7 and P11, expression was observed within the acinar region of the gland. Once the gland was fully developed (adult), Pax6 expression was significantly reduced within the acini. MG = meibomian gland; HF = hair follicle; EP = epidermis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4764607&req=5

f2: Differential expression of Pax6. Ten-micron horizontal cryosections of the eyelid were examined for Pax6 expression. The developing meibomian gland expressed cytoplasmic Pax6 as early as P4 in the elongating epithelial cord. At P7 and P11, expression was observed within the acinar region of the gland. Once the gland was fully developed (adult), Pax6 expression was significantly reduced within the acini. MG = meibomian gland; HF = hair follicle; EP = epidermis.
Mentions: Pax6 is a transcription factor expressed by ectodermal tissue and plays a critical role in the development and morphogenesis of many ocular tissues. Cytoplasmic Pax6 expression was present in the acinar cells throughout all stages of meibomian gland morphogenesis, but this expression was significantly reduced in the adult meibomian glands (Figure 2).

Bottom Line: This colocalization region decreased as the gland developed becoming restricted to the central duct near the opening to the acini in the fully developed gland.This unique group of cells was located in the central duct with a concentration near the ductule orifice.The expression of these cells reduced during meibomian gland morphogenesis and may play a role in the development and homeostasis of the gland.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Cincinnati, Cincinnati OH.

ABSTRACT

Purpose: To identify the lineage that contributes to the morphogenesis of the meibomian gland.

Methods: To examine which cell lineage gives rise to the meibomian gland, the expression of Pax6 as well as that of various cytokeratin markers, including keratin 14 (Krt14), Krt15, Krt4, and Krt10, was examined with immunofluorescent staining of C57BL/6J mouse eyelids from P2 to P11 pups and adult mice.

Results: Pax6 was localized to the cytoplasm within the acinar region of the meibomian glands during morphogenesis but was absent in the fully developed gland. Keratin 14 was expressed throughout the gland at all stages whereas keratin 15 was absent at all stages. Keratin 4, a marker of mucosal lineage, was present throughout the gland and was colocalized with keratin 10 (epidermal lineage marker) in the developing duct at P4. This colocalization region decreased as the gland developed becoming restricted to the central duct near the opening to the acini in the fully developed gland.

Conclusions: We identified a unique cell lineage that expresses markers characteristic of mucosal and epidermal epithelia during meibomian gland morphogenesis. This unique group of cells was located in the central duct with a concentration near the ductule orifice. The expression of these cells reduced during meibomian gland morphogenesis and may play a role in the development and homeostasis of the gland.

No MeSH data available.


Related in: MedlinePlus