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Untwisting the Caenorhabditis elegans embryo.

Christensen RP, Bokinsky A, Santella A, Wu Y, Marquina-Solis J, Guo M, Kovacevic I, Kumar A, Winter PW, Tashakkori N, McCreedy E, Liu H, McAuliffe M, Mohler W, Colón-Ramos DA, Bao Z, Shroff H - Elife (2015)

Bottom Line: However, studies of single cell development have largely been conducted in fixed or pre-twitching live embryos, because of technical difficulties associated with embryo movement in late embryogenesis.The detailed positional information we obtained enabled us to develop a composite model showing movement of these cells and neurites in an 'average' worm embryo.The untwisting and cell tracking capabilities of our method provide a foundation on which to catalog C. elegans neurodevelopment, allowing interrogation of developmental events in previously inaccessible periods of embryogenesis.

View Article: PubMed Central - PubMed

Affiliation: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, United States.

ABSTRACT
The nematode Caenorhabditis elegans possesses a simple embryonic nervous system with few enough neurons that the growth of each cell could be followed to provide a systems-level view of development. However, studies of single cell development have largely been conducted in fixed or pre-twitching live embryos, because of technical difficulties associated with embryo movement in late embryogenesis. We present open-source untwisting and annotation software (http://mipav.cit.nih.gov/plugin_jws/mipav_worm_plugin.php) that allows the investigation of neurodevelopmental events in late embryogenesis and apply it to track the 3D positions of seam cell nuclei, neurons, and neurites in multiple elongating embryos. We also provide a tutorial describing how to use the software (Supplementary file 1) and a detailed description of the untwisting algorithm (Appendix). The detailed positional information we obtained enabled us to develop a composite model showing movement of these cells and neurites in an 'average' worm embryo. The untwisting and cell tracking capabilities of our method provide a foundation on which to catalog C. elegans neurodevelopment, allowing interrogation of developmental events in previously inaccessible periods of embryogenesis.

No MeSH data available.


Related in: MedlinePlus

Annotations added to the worm volume labeling parts of the neuron.DOI:http://dx.doi.org/10.7554/eLife.10070.048
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fig16: Annotations added to the worm volume labeling parts of the neuron.DOI:http://dx.doi.org/10.7554/eLife.10070.048

Mentions: The program also features the ability to add annotation points, for which the program records the X, Y, and Z-coordinate in relation to a user-defined origin point (typically the nose), and then outputs that location information in a spreadsheet file. The annotation points can be used to define the position of a cell or cellular structure in each worm volume so spatial displacement can be measured over time. Thus a user can examine the position of a specific cell or structure of interest in multiple worm volumes, to determine how stereotyped the position of that cell is during embryo development (Appendix 1—figure 10).10.7554/eLife.10070.048Appendix 1—figure 10.Annotations added to the worm volume labeling parts of the neuron.


Untwisting the Caenorhabditis elegans embryo.

Christensen RP, Bokinsky A, Santella A, Wu Y, Marquina-Solis J, Guo M, Kovacevic I, Kumar A, Winter PW, Tashakkori N, McCreedy E, Liu H, McAuliffe M, Mohler W, Colón-Ramos DA, Bao Z, Shroff H - Elife (2015)

Annotations added to the worm volume labeling parts of the neuron.DOI:http://dx.doi.org/10.7554/eLife.10070.048
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4764590&req=5

fig16: Annotations added to the worm volume labeling parts of the neuron.DOI:http://dx.doi.org/10.7554/eLife.10070.048
Mentions: The program also features the ability to add annotation points, for which the program records the X, Y, and Z-coordinate in relation to a user-defined origin point (typically the nose), and then outputs that location information in a spreadsheet file. The annotation points can be used to define the position of a cell or cellular structure in each worm volume so spatial displacement can be measured over time. Thus a user can examine the position of a specific cell or structure of interest in multiple worm volumes, to determine how stereotyped the position of that cell is during embryo development (Appendix 1—figure 10).10.7554/eLife.10070.048Appendix 1—figure 10.Annotations added to the worm volume labeling parts of the neuron.

Bottom Line: However, studies of single cell development have largely been conducted in fixed or pre-twitching live embryos, because of technical difficulties associated with embryo movement in late embryogenesis.The detailed positional information we obtained enabled us to develop a composite model showing movement of these cells and neurites in an 'average' worm embryo.The untwisting and cell tracking capabilities of our method provide a foundation on which to catalog C. elegans neurodevelopment, allowing interrogation of developmental events in previously inaccessible periods of embryogenesis.

View Article: PubMed Central - PubMed

Affiliation: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, United States.

ABSTRACT
The nematode Caenorhabditis elegans possesses a simple embryonic nervous system with few enough neurons that the growth of each cell could be followed to provide a systems-level view of development. However, studies of single cell development have largely been conducted in fixed or pre-twitching live embryos, because of technical difficulties associated with embryo movement in late embryogenesis. We present open-source untwisting and annotation software (http://mipav.cit.nih.gov/plugin_jws/mipav_worm_plugin.php) that allows the investigation of neurodevelopmental events in late embryogenesis and apply it to track the 3D positions of seam cell nuclei, neurons, and neurites in multiple elongating embryos. We also provide a tutorial describing how to use the software (Supplementary file 1) and a detailed description of the untwisting algorithm (Appendix). The detailed positional information we obtained enabled us to develop a composite model showing movement of these cells and neurites in an 'average' worm embryo. The untwisting and cell tracking capabilities of our method provide a foundation on which to catalog C. elegans neurodevelopment, allowing interrogation of developmental events in previously inaccessible periods of embryogenesis.

No MeSH data available.


Related in: MedlinePlus