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MicroRNA-410-5p as a potential serum biomarker for the diagnosis of prostate cancer.

Wang J, Ye H, Zhang D, Hu Y, Yu X, Wang L, Zuo C, Yu Y, Xu G, Liu S - Cancer Cell Int. (2016)

Bottom Line: Plasma miRNAs are ideal biomarkers in heart failure, diabetes and other disease.Circulating miRNA-410-5p (miR-410-5p) level was significantly higher in the PCa patients than in healthy controls or non-cancer patients.The serum miR-410-5p level is a potential biomarker for the diagnosis of PCa.

View Article: PubMed Central - PubMed

Affiliation: Clinical Research Center, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.

ABSTRACT

Background: Prostate cancer (PCa) remains to be a diagnostic challenge due to its variable presentation and the lack of reliable diagnosis tool. MicroRNAs (miRNAs) regulate gene in extensive range of pathophysiologic processes. Plasma miRNAs are ideal biomarkers in heart failure, diabetes and other disease. However, using circulating miRNAs as biomarkers for the diagnosis of PCa is still unknown.

Methods: 149 PCa patients, 57 healthy controls, and 121 non-cancer patients (benign prostatic hyperplasia and other urinary diseases) were enrolled in this study. The reverse transcription of miRNA and SYBR-Green-based double standards curve miRNA quantitative polymerase chain reactions (qPCR) were used to evaluate the dysregulated miR-410-5p. Receiver operator characteristic (ROC) curve analysis was used to evaluate the diagnostic accuracy of miR-410-5p identified as the alternative biomarker.

Results: Circulating miRNA-410-5p (miR-410-5p) level was significantly higher in the PCa patients than in healthy controls or non-cancer patients. ROC curve analysis showed that plasma miR-410-5p was a specific diagnostic biomarker of PCa with an area under curve(AUC) of 0.8097 (95 % confidence interval, 0.7371-0.8823; P < 0.001).

Conclusions: The serum miR-410-5p level is a potential biomarker for the diagnosis of PCa.

No MeSH data available.


Related in: MedlinePlus

Serum miR-410-5p levels in PCa patients and comparison test with miR-1228 and let-7c. a The relative serum miR-410-5p level in 115 good prognosis patient (no recurrence) and 34 PCa patients with poor prognosis. The expression levels of miR-410-5p was normalized by U6. The value of miR-410-5p was provided above the histogram. **, P < 0.01. b The relative serum level of let-7c, miR-410-5p, and miR1228 in 73 samples (Including 34 randomly selected PCa patients, 25 BPH patients and 14 healthy controls). The expression levels were normalized by U6. The value of miRNAs level and p value were provided above the histogram
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Fig2: Serum miR-410-5p levels in PCa patients and comparison test with miR-1228 and let-7c. a The relative serum miR-410-5p level in 115 good prognosis patient (no recurrence) and 34 PCa patients with poor prognosis. The expression levels of miR-410-5p was normalized by U6. The value of miR-410-5p was provided above the histogram. **, P < 0.01. b The relative serum level of let-7c, miR-410-5p, and miR1228 in 73 samples (Including 34 randomly selected PCa patients, 25 BPH patients and 14 healthy controls). The expression levels were normalized by U6. The value of miRNAs level and p value were provided above the histogram

Mentions: The plasma miR-410-5p level was increased in the PCa group compared to both the non-cancer group and healthy controls (Fig. 1a). However, plasma miR-410-5p was also higher in the serums of PCa patients without recurrence than in the serums of PCa patients with poor prognosis (Fig. 2a), indicating that miR-410-5p may be a stable biomarker to evaluate the prognosis of PCa. To confirm that the assay is reproducible, all tests were repeated twice. It must be confirmed that no remarkable difference in the content of miR-410-5p was found between three tests of one sample. We also compared the plasma miR-410-5p level between high-intermediate risk PCa and low-risk PCa and found that the miR-410-5p level was significantly higher in the low-risk patients compared to high-intermediate-risk PCa patients (Fig. 1c).Fig. 2


MicroRNA-410-5p as a potential serum biomarker for the diagnosis of prostate cancer.

Wang J, Ye H, Zhang D, Hu Y, Yu X, Wang L, Zuo C, Yu Y, Xu G, Liu S - Cancer Cell Int. (2016)

Serum miR-410-5p levels in PCa patients and comparison test with miR-1228 and let-7c. a The relative serum miR-410-5p level in 115 good prognosis patient (no recurrence) and 34 PCa patients with poor prognosis. The expression levels of miR-410-5p was normalized by U6. The value of miR-410-5p was provided above the histogram. **, P < 0.01. b The relative serum level of let-7c, miR-410-5p, and miR1228 in 73 samples (Including 34 randomly selected PCa patients, 25 BPH patients and 14 healthy controls). The expression levels were normalized by U6. The value of miRNAs level and p value were provided above the histogram
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4759854&req=5

Fig2: Serum miR-410-5p levels in PCa patients and comparison test with miR-1228 and let-7c. a The relative serum miR-410-5p level in 115 good prognosis patient (no recurrence) and 34 PCa patients with poor prognosis. The expression levels of miR-410-5p was normalized by U6. The value of miR-410-5p was provided above the histogram. **, P < 0.01. b The relative serum level of let-7c, miR-410-5p, and miR1228 in 73 samples (Including 34 randomly selected PCa patients, 25 BPH patients and 14 healthy controls). The expression levels were normalized by U6. The value of miRNAs level and p value were provided above the histogram
Mentions: The plasma miR-410-5p level was increased in the PCa group compared to both the non-cancer group and healthy controls (Fig. 1a). However, plasma miR-410-5p was also higher in the serums of PCa patients without recurrence than in the serums of PCa patients with poor prognosis (Fig. 2a), indicating that miR-410-5p may be a stable biomarker to evaluate the prognosis of PCa. To confirm that the assay is reproducible, all tests were repeated twice. It must be confirmed that no remarkable difference in the content of miR-410-5p was found between three tests of one sample. We also compared the plasma miR-410-5p level between high-intermediate risk PCa and low-risk PCa and found that the miR-410-5p level was significantly higher in the low-risk patients compared to high-intermediate-risk PCa patients (Fig. 1c).Fig. 2

Bottom Line: Plasma miRNAs are ideal biomarkers in heart failure, diabetes and other disease.Circulating miRNA-410-5p (miR-410-5p) level was significantly higher in the PCa patients than in healthy controls or non-cancer patients.The serum miR-410-5p level is a potential biomarker for the diagnosis of PCa.

View Article: PubMed Central - PubMed

Affiliation: Clinical Research Center, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.

ABSTRACT

Background: Prostate cancer (PCa) remains to be a diagnostic challenge due to its variable presentation and the lack of reliable diagnosis tool. MicroRNAs (miRNAs) regulate gene in extensive range of pathophysiologic processes. Plasma miRNAs are ideal biomarkers in heart failure, diabetes and other disease. However, using circulating miRNAs as biomarkers for the diagnosis of PCa is still unknown.

Methods: 149 PCa patients, 57 healthy controls, and 121 non-cancer patients (benign prostatic hyperplasia and other urinary diseases) were enrolled in this study. The reverse transcription of miRNA and SYBR-Green-based double standards curve miRNA quantitative polymerase chain reactions (qPCR) were used to evaluate the dysregulated miR-410-5p. Receiver operator characteristic (ROC) curve analysis was used to evaluate the diagnostic accuracy of miR-410-5p identified as the alternative biomarker.

Results: Circulating miRNA-410-5p (miR-410-5p) level was significantly higher in the PCa patients than in healthy controls or non-cancer patients. ROC curve analysis showed that plasma miR-410-5p was a specific diagnostic biomarker of PCa with an area under curve(AUC) of 0.8097 (95 % confidence interval, 0.7371-0.8823; P < 0.001).

Conclusions: The serum miR-410-5p level is a potential biomarker for the diagnosis of PCa.

No MeSH data available.


Related in: MedlinePlus