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MicroRNA-410-5p as a potential serum biomarker for the diagnosis of prostate cancer.

Wang J, Ye H, Zhang D, Hu Y, Yu X, Wang L, Zuo C, Yu Y, Xu G, Liu S - Cancer Cell Int. (2016)

Bottom Line: Plasma miRNAs are ideal biomarkers in heart failure, diabetes and other disease.Circulating miRNA-410-5p (miR-410-5p) level was significantly higher in the PCa patients than in healthy controls or non-cancer patients.The serum miR-410-5p level is a potential biomarker for the diagnosis of PCa.

View Article: PubMed Central - PubMed

Affiliation: Clinical Research Center, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.

ABSTRACT

Background: Prostate cancer (PCa) remains to be a diagnostic challenge due to its variable presentation and the lack of reliable diagnosis tool. MicroRNAs (miRNAs) regulate gene in extensive range of pathophysiologic processes. Plasma miRNAs are ideal biomarkers in heart failure, diabetes and other disease. However, using circulating miRNAs as biomarkers for the diagnosis of PCa is still unknown.

Methods: 149 PCa patients, 57 healthy controls, and 121 non-cancer patients (benign prostatic hyperplasia and other urinary diseases) were enrolled in this study. The reverse transcription of miRNA and SYBR-Green-based double standards curve miRNA quantitative polymerase chain reactions (qPCR) were used to evaluate the dysregulated miR-410-5p. Receiver operator characteristic (ROC) curve analysis was used to evaluate the diagnostic accuracy of miR-410-5p identified as the alternative biomarker.

Results: Circulating miRNA-410-5p (miR-410-5p) level was significantly higher in the PCa patients than in healthy controls or non-cancer patients. ROC curve analysis showed that plasma miR-410-5p was a specific diagnostic biomarker of PCa with an area under curve(AUC) of 0.8097 (95 % confidence interval, 0.7371-0.8823; P < 0.001).

Conclusions: The serum miR-410-5p level is a potential biomarker for the diagnosis of PCa.

No MeSH data available.


Related in: MedlinePlus

Serum miR-410-5p levels in different groups. a The relative serum miR-410-5p levels in 20 healthy controls and 51 PCa patients. The value of miR-410-5p was provided above the histogram. **, P < 0.01. b The Serum miR-410-5p level in 57 healthy volunteers, 121 non-PCA controls (include 81 patients with BPH and 40 patients with other urinary diseases) and 149 PCA patients. The value of miR-410-5p was provided above the histogram. **, P < 0.01. c The Serum miR-410 levels in 65 low-risk PCa patients (Gleason level <7) and 84 high-intermediate-risk PCa (Gleason level >=7). The value of miR-410-5p was provided above the histogram. **, P < 0.01
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Fig1: Serum miR-410-5p levels in different groups. a The relative serum miR-410-5p levels in 20 healthy controls and 51 PCa patients. The value of miR-410-5p was provided above the histogram. **, P < 0.01. b The Serum miR-410-5p level in 57 healthy volunteers, 121 non-PCA controls (include 81 patients with BPH and 40 patients with other urinary diseases) and 149 PCA patients. The value of miR-410-5p was provided above the histogram. **, P < 0.01. c The Serum miR-410 levels in 65 low-risk PCa patients (Gleason level <7) and 84 high-intermediate-risk PCa (Gleason level >=7). The value of miR-410-5p was provided above the histogram. **, P < 0.01

Mentions: Plasma miR-410-5p were identified as the biomarker in 57 PCa patients from 14 healthy controls (Fig. 1a). The elevation of miR-410-5p was validated using double standard curve qRT-PCR in 149 PCa patients and 178 non-cancer controls (Fig. 1b). The basic clinical characteristics of PCa patients and non-cancer patients are shown in the Table 1. It showed that there were no remarkable differences in age between the PCa and non-cancer groups. These data have been normalized by double standard curves, a widely used normalized method for qPCR that was also confirmed in our other research. Moreover, we compared the difference in miR-410-5p content between 85 high-intermediate risk PCa patients and 64 low-risk PCa patients and obtained the same differences in miR-410-5p expression (Fig. 1c), which further supports that miR-410-5p is a stable biomarker for PCa.Fig. 1


MicroRNA-410-5p as a potential serum biomarker for the diagnosis of prostate cancer.

Wang J, Ye H, Zhang D, Hu Y, Yu X, Wang L, Zuo C, Yu Y, Xu G, Liu S - Cancer Cell Int. (2016)

Serum miR-410-5p levels in different groups. a The relative serum miR-410-5p levels in 20 healthy controls and 51 PCa patients. The value of miR-410-5p was provided above the histogram. **, P < 0.01. b The Serum miR-410-5p level in 57 healthy volunteers, 121 non-PCA controls (include 81 patients with BPH and 40 patients with other urinary diseases) and 149 PCA patients. The value of miR-410-5p was provided above the histogram. **, P < 0.01. c The Serum miR-410 levels in 65 low-risk PCa patients (Gleason level <7) and 84 high-intermediate-risk PCa (Gleason level >=7). The value of miR-410-5p was provided above the histogram. **, P < 0.01
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4759854&req=5

Fig1: Serum miR-410-5p levels in different groups. a The relative serum miR-410-5p levels in 20 healthy controls and 51 PCa patients. The value of miR-410-5p was provided above the histogram. **, P < 0.01. b The Serum miR-410-5p level in 57 healthy volunteers, 121 non-PCA controls (include 81 patients with BPH and 40 patients with other urinary diseases) and 149 PCA patients. The value of miR-410-5p was provided above the histogram. **, P < 0.01. c The Serum miR-410 levels in 65 low-risk PCa patients (Gleason level <7) and 84 high-intermediate-risk PCa (Gleason level >=7). The value of miR-410-5p was provided above the histogram. **, P < 0.01
Mentions: Plasma miR-410-5p were identified as the biomarker in 57 PCa patients from 14 healthy controls (Fig. 1a). The elevation of miR-410-5p was validated using double standard curve qRT-PCR in 149 PCa patients and 178 non-cancer controls (Fig. 1b). The basic clinical characteristics of PCa patients and non-cancer patients are shown in the Table 1. It showed that there were no remarkable differences in age between the PCa and non-cancer groups. These data have been normalized by double standard curves, a widely used normalized method for qPCR that was also confirmed in our other research. Moreover, we compared the difference in miR-410-5p content between 85 high-intermediate risk PCa patients and 64 low-risk PCa patients and obtained the same differences in miR-410-5p expression (Fig. 1c), which further supports that miR-410-5p is a stable biomarker for PCa.Fig. 1

Bottom Line: Plasma miRNAs are ideal biomarkers in heart failure, diabetes and other disease.Circulating miRNA-410-5p (miR-410-5p) level was significantly higher in the PCa patients than in healthy controls or non-cancer patients.The serum miR-410-5p level is a potential biomarker for the diagnosis of PCa.

View Article: PubMed Central - PubMed

Affiliation: Clinical Research Center, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.

ABSTRACT

Background: Prostate cancer (PCa) remains to be a diagnostic challenge due to its variable presentation and the lack of reliable diagnosis tool. MicroRNAs (miRNAs) regulate gene in extensive range of pathophysiologic processes. Plasma miRNAs are ideal biomarkers in heart failure, diabetes and other disease. However, using circulating miRNAs as biomarkers for the diagnosis of PCa is still unknown.

Methods: 149 PCa patients, 57 healthy controls, and 121 non-cancer patients (benign prostatic hyperplasia and other urinary diseases) were enrolled in this study. The reverse transcription of miRNA and SYBR-Green-based double standards curve miRNA quantitative polymerase chain reactions (qPCR) were used to evaluate the dysregulated miR-410-5p. Receiver operator characteristic (ROC) curve analysis was used to evaluate the diagnostic accuracy of miR-410-5p identified as the alternative biomarker.

Results: Circulating miRNA-410-5p (miR-410-5p) level was significantly higher in the PCa patients than in healthy controls or non-cancer patients. ROC curve analysis showed that plasma miR-410-5p was a specific diagnostic biomarker of PCa with an area under curve(AUC) of 0.8097 (95 % confidence interval, 0.7371-0.8823; P < 0.001).

Conclusions: The serum miR-410-5p level is a potential biomarker for the diagnosis of PCa.

No MeSH data available.


Related in: MedlinePlus