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Herbal preparation (HemoHIM) enhanced functional maturation of bone marrow-derived dendritic cells mediated toll-like receptor 4.

Lee SJ, Kim JJ, Kang KY, Hwang YH, Jeong GY, Jo SK, Jung U, Park HR, Yee ST - BMC Complement Altern Med (2016)

Bottom Line: HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously.Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4(+) and CD8(+) T cell responses.These results suggest that HemoHIM has the potential to mediate DC immune responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon, 549-742, Republic of Korea. 6525140@hanmail.net.

ABSTRACT

Background: HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously. DCs are a unique group of white blood cells that initiate primary immune responses by capturing, processing, and presenting antigens to T cells.

Results: In the present study, we investigated the effect of HemoHIM on the functional and phenotypic maturation of murine bone marrow-derived dendritic cells (BMDCs) both in vitro and in vivo. The expression of co-stimulatory molecules (CD40, CD80, CD86, MHC I, and MHC II) and the production of cytokines (IL-1β, IL-6, IL-12p70, and TNF-α) were increased by HemoHIM in BMDCs. Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4(+) and CD8(+) T cell responses.

Conclusions: Our findings demonstrated that HemoHIM induces TLR4-mediated BMDCs functional and phenotypic maturation through in vivo and in vitro. And our study showed the antigen-presenting ability that HemoHIM-treated mature BMDCs increase CD4(+) and CD8(+) T cell responses by in vitro. These results suggest that HemoHIM has the potential to mediate DC immune responses.

No MeSH data available.


HemoHIM-treated BMDCs induced the activation of allogeneic CD8+ T cells derived from C57BL/6 OT-1 mice. CD8+ T cells were co-cultured for 24 h with C57BL/6-derived BMDCs that were treated with LPS (1 μg/ml) or HemoHIM (100 μg/ml). a Flow cytometry of intracellular IFN-γ staining in CD8+ T cells. b Culture supernatants were harvested after 24 h, and cytokine levels were measured by ELISA. The results are representative of three experiments. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. OVA257-264-specific T cells co-cultured with untreated BMDCs. +, p < 0.05, ++, p < 0.01, and +++, p < 0.001 vs. OVA257-264-specific T cells co-cultured with OVA257-264-pulsed BMDCs. Not detection (N.D) showed less than 10 pg/ml of cytokine secretion in this experiment
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Fig6: HemoHIM-treated BMDCs induced the activation of allogeneic CD8+ T cells derived from C57BL/6 OT-1 mice. CD8+ T cells were co-cultured for 24 h with C57BL/6-derived BMDCs that were treated with LPS (1 μg/ml) or HemoHIM (100 μg/ml). a Flow cytometry of intracellular IFN-γ staining in CD8+ T cells. b Culture supernatants were harvested after 24 h, and cytokine levels were measured by ELISA. The results are representative of three experiments. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. OVA257-264-specific T cells co-cultured with untreated BMDCs. +, p < 0.05, ++, p < 0.01, and +++, p < 0.001 vs. OVA257-264-specific T cells co-cultured with OVA257-264-pulsed BMDCs. Not detection (N.D) showed less than 10 pg/ml of cytokine secretion in this experiment

Mentions: The presentation of captured antigens to cytotoxic CD8+ T cells is important for the induction of anti-tumor immunity. To examine the ability of HemoHIM-treated BMDCs to increase antigen-presentation to CD8+ T cells, we used OT-1 mice. CFSE-labeled CD8+ T cells were co-cultured with untreated BMDCs pulsed with OVA257-264 or treated BMDCs (LPS or HemoHIM) pulsed with OVA257-264. CD8+ T cells co-cultured with HemoHIM-treated BMDCs proliferated more than CD8+ T cells co-cultured with untreated BMDCs (Fig. 5). Furthermore, CD8+ T cells co-cultured with HemoHIM-treated BMDCs produced significantly more IFN-γ than those co-cultured with untreated BMDCs, presumably due to the secretion of intracellular cytokines (Fig. 6a) and the production (Fig. 6b) of cytokines. These results supported the notion that HemoHIM enhances CD8+ T cell responses by activating DCs.Fig. 5


Herbal preparation (HemoHIM) enhanced functional maturation of bone marrow-derived dendritic cells mediated toll-like receptor 4.

Lee SJ, Kim JJ, Kang KY, Hwang YH, Jeong GY, Jo SK, Jung U, Park HR, Yee ST - BMC Complement Altern Med (2016)

HemoHIM-treated BMDCs induced the activation of allogeneic CD8+ T cells derived from C57BL/6 OT-1 mice. CD8+ T cells were co-cultured for 24 h with C57BL/6-derived BMDCs that were treated with LPS (1 μg/ml) or HemoHIM (100 μg/ml). a Flow cytometry of intracellular IFN-γ staining in CD8+ T cells. b Culture supernatants were harvested after 24 h, and cytokine levels were measured by ELISA. The results are representative of three experiments. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. OVA257-264-specific T cells co-cultured with untreated BMDCs. +, p < 0.05, ++, p < 0.01, and +++, p < 0.001 vs. OVA257-264-specific T cells co-cultured with OVA257-264-pulsed BMDCs. Not detection (N.D) showed less than 10 pg/ml of cytokine secretion in this experiment
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4759761&req=5

Fig6: HemoHIM-treated BMDCs induced the activation of allogeneic CD8+ T cells derived from C57BL/6 OT-1 mice. CD8+ T cells were co-cultured for 24 h with C57BL/6-derived BMDCs that were treated with LPS (1 μg/ml) or HemoHIM (100 μg/ml). a Flow cytometry of intracellular IFN-γ staining in CD8+ T cells. b Culture supernatants were harvested after 24 h, and cytokine levels were measured by ELISA. The results are representative of three experiments. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. OVA257-264-specific T cells co-cultured with untreated BMDCs. +, p < 0.05, ++, p < 0.01, and +++, p < 0.001 vs. OVA257-264-specific T cells co-cultured with OVA257-264-pulsed BMDCs. Not detection (N.D) showed less than 10 pg/ml of cytokine secretion in this experiment
Mentions: The presentation of captured antigens to cytotoxic CD8+ T cells is important for the induction of anti-tumor immunity. To examine the ability of HemoHIM-treated BMDCs to increase antigen-presentation to CD8+ T cells, we used OT-1 mice. CFSE-labeled CD8+ T cells were co-cultured with untreated BMDCs pulsed with OVA257-264 or treated BMDCs (LPS or HemoHIM) pulsed with OVA257-264. CD8+ T cells co-cultured with HemoHIM-treated BMDCs proliferated more than CD8+ T cells co-cultured with untreated BMDCs (Fig. 5). Furthermore, CD8+ T cells co-cultured with HemoHIM-treated BMDCs produced significantly more IFN-γ than those co-cultured with untreated BMDCs, presumably due to the secretion of intracellular cytokines (Fig. 6a) and the production (Fig. 6b) of cytokines. These results supported the notion that HemoHIM enhances CD8+ T cell responses by activating DCs.Fig. 5

Bottom Line: HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously.Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4(+) and CD8(+) T cell responses.These results suggest that HemoHIM has the potential to mediate DC immune responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon, 549-742, Republic of Korea. 6525140@hanmail.net.

ABSTRACT

Background: HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously. DCs are a unique group of white blood cells that initiate primary immune responses by capturing, processing, and presenting antigens to T cells.

Results: In the present study, we investigated the effect of HemoHIM on the functional and phenotypic maturation of murine bone marrow-derived dendritic cells (BMDCs) both in vitro and in vivo. The expression of co-stimulatory molecules (CD40, CD80, CD86, MHC I, and MHC II) and the production of cytokines (IL-1β, IL-6, IL-12p70, and TNF-α) were increased by HemoHIM in BMDCs. Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4(+) and CD8(+) T cell responses.

Conclusions: Our findings demonstrated that HemoHIM induces TLR4-mediated BMDCs functional and phenotypic maturation through in vivo and in vitro. And our study showed the antigen-presenting ability that HemoHIM-treated mature BMDCs increase CD4(+) and CD8(+) T cell responses by in vitro. These results suggest that HemoHIM has the potential to mediate DC immune responses.

No MeSH data available.