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Herbal preparation (HemoHIM) enhanced functional maturation of bone marrow-derived dendritic cells mediated toll-like receptor 4.

Lee SJ, Kim JJ, Kang KY, Hwang YH, Jeong GY, Jo SK, Jung U, Park HR, Yee ST - BMC Complement Altern Med (2016)

Bottom Line: HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously.Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4(+) and CD8(+) T cell responses.These results suggest that HemoHIM has the potential to mediate DC immune responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon, 549-742, Republic of Korea. 6525140@hanmail.net.

ABSTRACT

Background: HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously. DCs are a unique group of white blood cells that initiate primary immune responses by capturing, processing, and presenting antigens to T cells.

Results: In the present study, we investigated the effect of HemoHIM on the functional and phenotypic maturation of murine bone marrow-derived dendritic cells (BMDCs) both in vitro and in vivo. The expression of co-stimulatory molecules (CD40, CD80, CD86, MHC I, and MHC II) and the production of cytokines (IL-1β, IL-6, IL-12p70, and TNF-α) were increased by HemoHIM in BMDCs. Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4(+) and CD8(+) T cell responses.

Conclusions: Our findings demonstrated that HemoHIM induces TLR4-mediated BMDCs functional and phenotypic maturation through in vivo and in vitro. And our study showed the antigen-presenting ability that HemoHIM-treated mature BMDCs increase CD4(+) and CD8(+) T cell responses by in vitro. These results suggest that HemoHIM has the potential to mediate DC immune responses.

No MeSH data available.


Related in: MedlinePlus

HemoHIM-treated BMDCs induced the activations of allogeneic CD4+ T cells derived from BALB/c mice. a CD4+ T cells were co-cultured for 48 h with C57BL/6-derived BMDCs that were treated with LPS (1 μg/ml) or HemoHIM (100 μg/ml). The proliferation of allogeneic CD4+ T cells was assessed using a Bromo-kit. b Culture supernatants were harvested after 48 h, and cytokine levels were measured by ELISA. The results are representative of three experiments. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. CD4+ T cells co-cultured with untreated BMDCs. Not detection (N.D) showed less than 10 pg/ml of cytokine secretion in this experiment
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Fig3: HemoHIM-treated BMDCs induced the activations of allogeneic CD4+ T cells derived from BALB/c mice. a CD4+ T cells were co-cultured for 48 h with C57BL/6-derived BMDCs that were treated with LPS (1 μg/ml) or HemoHIM (100 μg/ml). The proliferation of allogeneic CD4+ T cells was assessed using a Bromo-kit. b Culture supernatants were harvested after 48 h, and cytokine levels were measured by ELISA. The results are representative of three experiments. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. CD4+ T cells co-cultured with untreated BMDCs. Not detection (N.D) showed less than 10 pg/ml of cytokine secretion in this experiment

Mentions: To examine the ability of HemoHIM-treated BMDCs to increase CD4+ and CD8+ T cell responses, we performed an allogeneic MLR assay using BALB/c CD4+ T cells. CD4+ T cells were co-cultured with untreated BMDCs, LPS-treated BMDCs, or HemoHIM-treated BMDCs. After co-culture for 2 days, CD4+ T cells co-cultured with HemoHIM-treated BMDCs were found to induce more CD4+ T cell proliferation than CD4+ T cells co-cultured with untreated BMDCs (Fig. 3a). To determine whether HemoHIM-treated BMDCs modulate cytokine secretion during allogeneic T cell responses, we measured IL-2, IFN-γ, and IL-4 levels in supernatants after co-culture for 2 days. HemoHIM-treated BMDCs co-cultured with CD4+ T cells secreted higher levels of IFN-γ, IL-4, and IL-2 than untreated BMDCs (Fig. 3b). In addition, the proliferation and cytokine secretion of CD4+ T cells increased in accordance with the T cell to DC ratio, and IFN-γ production increased more than IL-4 production (Fig. 3b). These results suggest that the activation and maturation of BMDCs by HemoHIM activates T cells and enhances Th1 responses.Fig. 3


Herbal preparation (HemoHIM) enhanced functional maturation of bone marrow-derived dendritic cells mediated toll-like receptor 4.

Lee SJ, Kim JJ, Kang KY, Hwang YH, Jeong GY, Jo SK, Jung U, Park HR, Yee ST - BMC Complement Altern Med (2016)

HemoHIM-treated BMDCs induced the activations of allogeneic CD4+ T cells derived from BALB/c mice. a CD4+ T cells were co-cultured for 48 h with C57BL/6-derived BMDCs that were treated with LPS (1 μg/ml) or HemoHIM (100 μg/ml). The proliferation of allogeneic CD4+ T cells was assessed using a Bromo-kit. b Culture supernatants were harvested after 48 h, and cytokine levels were measured by ELISA. The results are representative of three experiments. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. CD4+ T cells co-cultured with untreated BMDCs. Not detection (N.D) showed less than 10 pg/ml of cytokine secretion in this experiment
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4759761&req=5

Fig3: HemoHIM-treated BMDCs induced the activations of allogeneic CD4+ T cells derived from BALB/c mice. a CD4+ T cells were co-cultured for 48 h with C57BL/6-derived BMDCs that were treated with LPS (1 μg/ml) or HemoHIM (100 μg/ml). The proliferation of allogeneic CD4+ T cells was assessed using a Bromo-kit. b Culture supernatants were harvested after 48 h, and cytokine levels were measured by ELISA. The results are representative of three experiments. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. CD4+ T cells co-cultured with untreated BMDCs. Not detection (N.D) showed less than 10 pg/ml of cytokine secretion in this experiment
Mentions: To examine the ability of HemoHIM-treated BMDCs to increase CD4+ and CD8+ T cell responses, we performed an allogeneic MLR assay using BALB/c CD4+ T cells. CD4+ T cells were co-cultured with untreated BMDCs, LPS-treated BMDCs, or HemoHIM-treated BMDCs. After co-culture for 2 days, CD4+ T cells co-cultured with HemoHIM-treated BMDCs were found to induce more CD4+ T cell proliferation than CD4+ T cells co-cultured with untreated BMDCs (Fig. 3a). To determine whether HemoHIM-treated BMDCs modulate cytokine secretion during allogeneic T cell responses, we measured IL-2, IFN-γ, and IL-4 levels in supernatants after co-culture for 2 days. HemoHIM-treated BMDCs co-cultured with CD4+ T cells secreted higher levels of IFN-γ, IL-4, and IL-2 than untreated BMDCs (Fig. 3b). In addition, the proliferation and cytokine secretion of CD4+ T cells increased in accordance with the T cell to DC ratio, and IFN-γ production increased more than IL-4 production (Fig. 3b). These results suggest that the activation and maturation of BMDCs by HemoHIM activates T cells and enhances Th1 responses.Fig. 3

Bottom Line: HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously.Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4(+) and CD8(+) T cell responses.These results suggest that HemoHIM has the potential to mediate DC immune responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon, 549-742, Republic of Korea. 6525140@hanmail.net.

ABSTRACT

Background: HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously. DCs are a unique group of white blood cells that initiate primary immune responses by capturing, processing, and presenting antigens to T cells.

Results: In the present study, we investigated the effect of HemoHIM on the functional and phenotypic maturation of murine bone marrow-derived dendritic cells (BMDCs) both in vitro and in vivo. The expression of co-stimulatory molecules (CD40, CD80, CD86, MHC I, and MHC II) and the production of cytokines (IL-1β, IL-6, IL-12p70, and TNF-α) were increased by HemoHIM in BMDCs. Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4(+) and CD8(+) T cell responses.

Conclusions: Our findings demonstrated that HemoHIM induces TLR4-mediated BMDCs functional and phenotypic maturation through in vivo and in vitro. And our study showed the antigen-presenting ability that HemoHIM-treated mature BMDCs increase CD4(+) and CD8(+) T cell responses by in vitro. These results suggest that HemoHIM has the potential to mediate DC immune responses.

No MeSH data available.


Related in: MedlinePlus