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Herbal preparation (HemoHIM) enhanced functional maturation of bone marrow-derived dendritic cells mediated toll-like receptor 4.

Lee SJ, Kim JJ, Kang KY, Hwang YH, Jeong GY, Jo SK, Jung U, Park HR, Yee ST - BMC Complement Altern Med (2016)

Bottom Line: HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously.Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4(+) and CD8(+) T cell responses.These results suggest that HemoHIM has the potential to mediate DC immune responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon, 549-742, Republic of Korea. 6525140@hanmail.net.

ABSTRACT

Background: HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously. DCs are a unique group of white blood cells that initiate primary immune responses by capturing, processing, and presenting antigens to T cells.

Results: In the present study, we investigated the effect of HemoHIM on the functional and phenotypic maturation of murine bone marrow-derived dendritic cells (BMDCs) both in vitro and in vivo. The expression of co-stimulatory molecules (CD40, CD80, CD86, MHC I, and MHC II) and the production of cytokines (IL-1β, IL-6, IL-12p70, and TNF-α) were increased by HemoHIM in BMDCs. Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4(+) and CD8(+) T cell responses.

Conclusions: Our findings demonstrated that HemoHIM induces TLR4-mediated BMDCs functional and phenotypic maturation through in vivo and in vitro. And our study showed the antigen-presenting ability that HemoHIM-treated mature BMDCs increase CD4(+) and CD8(+) T cell responses by in vitro. These results suggest that HemoHIM has the potential to mediate DC immune responses.

No MeSH data available.


HemoHIM was not cytotoxic to BMDCs. BMDCs were treated with the indicated concentrations (1 μg/ml, 10 μg/ml and 100 μg/ml) of HemoHIM for 24 h, stained with Annexin V-FITC and PI, and analyzed by flow cytometry. The results are representative of three experiments
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Fig1: HemoHIM was not cytotoxic to BMDCs. BMDCs were treated with the indicated concentrations (1 μg/ml, 10 μg/ml and 100 μg/ml) of HemoHIM for 24 h, stained with Annexin V-FITC and PI, and analyzed by flow cytometry. The results are representative of three experiments

Mentions: We investigated whether HemoHIM at different concentrations induces apoptosis in BMDCs. As shown in Fig. 1, HemoHIM did not induce BMDC apoptosis at any of the examined concentrations.Fig. 1


Herbal preparation (HemoHIM) enhanced functional maturation of bone marrow-derived dendritic cells mediated toll-like receptor 4.

Lee SJ, Kim JJ, Kang KY, Hwang YH, Jeong GY, Jo SK, Jung U, Park HR, Yee ST - BMC Complement Altern Med (2016)

HemoHIM was not cytotoxic to BMDCs. BMDCs were treated with the indicated concentrations (1 μg/ml, 10 μg/ml and 100 μg/ml) of HemoHIM for 24 h, stained with Annexin V-FITC and PI, and analyzed by flow cytometry. The results are representative of three experiments
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4759761&req=5

Fig1: HemoHIM was not cytotoxic to BMDCs. BMDCs were treated with the indicated concentrations (1 μg/ml, 10 μg/ml and 100 μg/ml) of HemoHIM for 24 h, stained with Annexin V-FITC and PI, and analyzed by flow cytometry. The results are representative of three experiments
Mentions: We investigated whether HemoHIM at different concentrations induces apoptosis in BMDCs. As shown in Fig. 1, HemoHIM did not induce BMDC apoptosis at any of the examined concentrations.Fig. 1

Bottom Line: HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously.Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4(+) and CD8(+) T cell responses.These results suggest that HemoHIM has the potential to mediate DC immune responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon, 549-742, Republic of Korea. 6525140@hanmail.net.

ABSTRACT

Background: HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously. DCs are a unique group of white blood cells that initiate primary immune responses by capturing, processing, and presenting antigens to T cells.

Results: In the present study, we investigated the effect of HemoHIM on the functional and phenotypic maturation of murine bone marrow-derived dendritic cells (BMDCs) both in vitro and in vivo. The expression of co-stimulatory molecules (CD40, CD80, CD86, MHC I, and MHC II) and the production of cytokines (IL-1β, IL-6, IL-12p70, and TNF-α) were increased by HemoHIM in BMDCs. Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4(+) and CD8(+) T cell responses.

Conclusions: Our findings demonstrated that HemoHIM induces TLR4-mediated BMDCs functional and phenotypic maturation through in vivo and in vitro. And our study showed the antigen-presenting ability that HemoHIM-treated mature BMDCs increase CD4(+) and CD8(+) T cell responses by in vitro. These results suggest that HemoHIM has the potential to mediate DC immune responses.

No MeSH data available.