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OCT-based label-free in vivo lymphangiography within human skin and areola.

Baran U, Qin W, Qi X, Kalkan G, Wang RK - Sci Rep (2016)

Bottom Line: OLAG enables rapid (~seconds) mapping of lymphatic networks, along with blood vessel networks, over 8 mm x 8 mm of human skin and 5 mm x 5 mm of human areola.Moreover, lymphatic system's response to inflammation within human skin is monitored throughout an acne lesion development over 7 days.The demonstrated results promise OLAG as a revolutionary tool in the clinical research and treatment of patients with pathologic conditions such as cancer, diabetes, and autoimmune diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Washington, Seattle, WA, USA.

ABSTRACT
Due to the limitations of current imaging techniques, visualization of lymphatic capillaries within tissue in vivo has been challenging. Here, we present a label-free high resolution optical coherence tomography (OCT) based lymphangiography (OLAG) within human skin in vivo. OLAG enables rapid (~seconds) mapping of lymphatic networks, along with blood vessel networks, over 8 mm x 8 mm of human skin and 5 mm x 5 mm of human areola. Moreover, lymphatic system's response to inflammation within human skin is monitored throughout an acne lesion development over 7 days. The demonstrated results promise OLAG as a revolutionary tool in the clinical research and treatment of patients with pathologic conditions such as cancer, diabetes, and autoimmune diseases.

No MeSH data available.


Related in: MedlinePlus

Monitoring blood and lymphatic vessels within inflamed human skin with 5 mm × 5 mm imaging area.En face depth-resolved MIP of OMAG and sMIP of OLAG images with 5 mm × 5 mm field of view are shown for day 1, 2, and 3 of acne lesion development. Blood vessels are color coded according to their position in depth and lymphatic vessels are shown in green. Imaged areas are pointed out on photographs of acne lesion. While circles are highlighting the areas with edema.
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f3: Monitoring blood and lymphatic vessels within inflamed human skin with 5 mm × 5 mm imaging area.En face depth-resolved MIP of OMAG and sMIP of OLAG images with 5 mm × 5 mm field of view are shown for day 1, 2, and 3 of acne lesion development. Blood vessels are color coded according to their position in depth and lymphatic vessels are shown in green. Imaged areas are pointed out on photographs of acne lesion. While circles are highlighting the areas with edema.

Mentions: During inflammation, the lymphatic drainage is activated along with the vascular remodelling. Therefore, the monitoring of a simple inflammation phenomenon could be a good surrogate for demonstrating the usefulness of the proposed OLAG technique. Here, human skin’s inflammatory state and its recovery are monitored for 7 days within an acne lesion using OMAG and OLAG and shown in Figs 3 and 4. In these figures, the results for certain days are presented in each row. Accordingly, photograph, depth-resolved MIP OMAG, and the OLAG images from various depths are included in this figure. Field of view for OCT imaging is 5 mm ×5 mm.


OCT-based label-free in vivo lymphangiography within human skin and areola.

Baran U, Qin W, Qi X, Kalkan G, Wang RK - Sci Rep (2016)

Monitoring blood and lymphatic vessels within inflamed human skin with 5 mm × 5 mm imaging area.En face depth-resolved MIP of OMAG and sMIP of OLAG images with 5 mm × 5 mm field of view are shown for day 1, 2, and 3 of acne lesion development. Blood vessels are color coded according to their position in depth and lymphatic vessels are shown in green. Imaged areas are pointed out on photographs of acne lesion. While circles are highlighting the areas with edema.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4759696&req=5

f3: Monitoring blood and lymphatic vessels within inflamed human skin with 5 mm × 5 mm imaging area.En face depth-resolved MIP of OMAG and sMIP of OLAG images with 5 mm × 5 mm field of view are shown for day 1, 2, and 3 of acne lesion development. Blood vessels are color coded according to their position in depth and lymphatic vessels are shown in green. Imaged areas are pointed out on photographs of acne lesion. While circles are highlighting the areas with edema.
Mentions: During inflammation, the lymphatic drainage is activated along with the vascular remodelling. Therefore, the monitoring of a simple inflammation phenomenon could be a good surrogate for demonstrating the usefulness of the proposed OLAG technique. Here, human skin’s inflammatory state and its recovery are monitored for 7 days within an acne lesion using OMAG and OLAG and shown in Figs 3 and 4. In these figures, the results for certain days are presented in each row. Accordingly, photograph, depth-resolved MIP OMAG, and the OLAG images from various depths are included in this figure. Field of view for OCT imaging is 5 mm ×5 mm.

Bottom Line: OLAG enables rapid (~seconds) mapping of lymphatic networks, along with blood vessel networks, over 8 mm x 8 mm of human skin and 5 mm x 5 mm of human areola.Moreover, lymphatic system's response to inflammation within human skin is monitored throughout an acne lesion development over 7 days.The demonstrated results promise OLAG as a revolutionary tool in the clinical research and treatment of patients with pathologic conditions such as cancer, diabetes, and autoimmune diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Washington, Seattle, WA, USA.

ABSTRACT
Due to the limitations of current imaging techniques, visualization of lymphatic capillaries within tissue in vivo has been challenging. Here, we present a label-free high resolution optical coherence tomography (OCT) based lymphangiography (OLAG) within human skin in vivo. OLAG enables rapid (~seconds) mapping of lymphatic networks, along with blood vessel networks, over 8 mm x 8 mm of human skin and 5 mm x 5 mm of human areola. Moreover, lymphatic system's response to inflammation within human skin is monitored throughout an acne lesion development over 7 days. The demonstrated results promise OLAG as a revolutionary tool in the clinical research and treatment of patients with pathologic conditions such as cancer, diabetes, and autoimmune diseases.

No MeSH data available.


Related in: MedlinePlus