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OCT-based label-free in vivo lymphangiography within human skin and areola.

Baran U, Qin W, Qi X, Kalkan G, Wang RK - Sci Rep (2016)

Bottom Line: OLAG enables rapid (~seconds) mapping of lymphatic networks, along with blood vessel networks, over 8 mm x 8 mm of human skin and 5 mm x 5 mm of human areola.Moreover, lymphatic system's response to inflammation within human skin is monitored throughout an acne lesion development over 7 days.The demonstrated results promise OLAG as a revolutionary tool in the clinical research and treatment of patients with pathologic conditions such as cancer, diabetes, and autoimmune diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Washington, Seattle, WA, USA.

ABSTRACT
Due to the limitations of current imaging techniques, visualization of lymphatic capillaries within tissue in vivo has been challenging. Here, we present a label-free high resolution optical coherence tomography (OCT) based lymphangiography (OLAG) within human skin in vivo. OLAG enables rapid (~seconds) mapping of lymphatic networks, along with blood vessel networks, over 8 mm x 8 mm of human skin and 5 mm x 5 mm of human areola. Moreover, lymphatic system's response to inflammation within human skin is monitored throughout an acne lesion development over 7 days. The demonstrated results promise OLAG as a revolutionary tool in the clinical research and treatment of patients with pathologic conditions such as cancer, diabetes, and autoimmune diseases.

No MeSH data available.


Related in: MedlinePlus

En face mapping of lymphatic vessels at different layers of human lower arm skin with 8 mm × 8 mm total imaging area.(a) OCT volumetric structural image overlaid with volumetric OMAG acquired within in vivo human lower arm skin. (b) OCT cross-sectional image taken from the OCT volumetric data, pointed with black line in (a). SC: Stratum Corneum, E: Epidermis, EDJ: Epidermal-dermal junction, D: Dermis. (c) Segmented and processed cross section of dermis, pointed out at (b) with white dashed lines. (d) En face OMAG images with depth-resolved MIP. (e) Sketch of human lower arm where OCT images are taken from. (f–h) En face sMIP of lymphatic vessels from 0–200 um of dermis (f), 200–400 um of dermis (g), and 400–600 um of dermis (h). Red arrows point out some of the lymphatic vessels seen as low-scattering (dark) regions. [Figure (e) art by Uktu Baran].
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f1: En face mapping of lymphatic vessels at different layers of human lower arm skin with 8 mm × 8 mm total imaging area.(a) OCT volumetric structural image overlaid with volumetric OMAG acquired within in vivo human lower arm skin. (b) OCT cross-sectional image taken from the OCT volumetric data, pointed with black line in (a). SC: Stratum Corneum, E: Epidermis, EDJ: Epidermal-dermal junction, D: Dermis. (c) Segmented and processed cross section of dermis, pointed out at (b) with white dashed lines. (d) En face OMAG images with depth-resolved MIP. (e) Sketch of human lower arm where OCT images are taken from. (f–h) En face sMIP of lymphatic vessels from 0–200 um of dermis (f), 200–400 um of dermis (g), and 400–600 um of dermis (h). Red arrows point out some of the lymphatic vessels seen as low-scattering (dark) regions. [Figure (e) art by Uktu Baran].

Mentions: Using OCT, we imaged the skin in the ventral side of the human lower arm, and presented the results in Fig. 1. To achieve a large field of view, the final enface image is formed by mosaicking 9 images, each with 3 mm × 3 mm area. Depth-resolved maximum intensity projection (MIP) OMAG image shows the blood vessel network within human arm skin in Fig. 1d. Figure 1c shows the cross-section of the dermis after the compensation and the segmentation. The lymphatic vessels in the corresponding portions of the dermis are presented with enface sMIP (see Supplementary Information) in Fig. 1f,g. The results shows that the lymphatic vessels are in smaller shapes in the top layers of dermis and it becomes larger in the deeper layer, which is consistent with the literature22. Unlike blood vessels, lymphatic vessels are loosely attached together.


OCT-based label-free in vivo lymphangiography within human skin and areola.

Baran U, Qin W, Qi X, Kalkan G, Wang RK - Sci Rep (2016)

En face mapping of lymphatic vessels at different layers of human lower arm skin with 8 mm × 8 mm total imaging area.(a) OCT volumetric structural image overlaid with volumetric OMAG acquired within in vivo human lower arm skin. (b) OCT cross-sectional image taken from the OCT volumetric data, pointed with black line in (a). SC: Stratum Corneum, E: Epidermis, EDJ: Epidermal-dermal junction, D: Dermis. (c) Segmented and processed cross section of dermis, pointed out at (b) with white dashed lines. (d) En face OMAG images with depth-resolved MIP. (e) Sketch of human lower arm where OCT images are taken from. (f–h) En face sMIP of lymphatic vessels from 0–200 um of dermis (f), 200–400 um of dermis (g), and 400–600 um of dermis (h). Red arrows point out some of the lymphatic vessels seen as low-scattering (dark) regions. [Figure (e) art by Uktu Baran].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4759696&req=5

f1: En face mapping of lymphatic vessels at different layers of human lower arm skin with 8 mm × 8 mm total imaging area.(a) OCT volumetric structural image overlaid with volumetric OMAG acquired within in vivo human lower arm skin. (b) OCT cross-sectional image taken from the OCT volumetric data, pointed with black line in (a). SC: Stratum Corneum, E: Epidermis, EDJ: Epidermal-dermal junction, D: Dermis. (c) Segmented and processed cross section of dermis, pointed out at (b) with white dashed lines. (d) En face OMAG images with depth-resolved MIP. (e) Sketch of human lower arm where OCT images are taken from. (f–h) En face sMIP of lymphatic vessels from 0–200 um of dermis (f), 200–400 um of dermis (g), and 400–600 um of dermis (h). Red arrows point out some of the lymphatic vessels seen as low-scattering (dark) regions. [Figure (e) art by Uktu Baran].
Mentions: Using OCT, we imaged the skin in the ventral side of the human lower arm, and presented the results in Fig. 1. To achieve a large field of view, the final enface image is formed by mosaicking 9 images, each with 3 mm × 3 mm area. Depth-resolved maximum intensity projection (MIP) OMAG image shows the blood vessel network within human arm skin in Fig. 1d. Figure 1c shows the cross-section of the dermis after the compensation and the segmentation. The lymphatic vessels in the corresponding portions of the dermis are presented with enface sMIP (see Supplementary Information) in Fig. 1f,g. The results shows that the lymphatic vessels are in smaller shapes in the top layers of dermis and it becomes larger in the deeper layer, which is consistent with the literature22. Unlike blood vessels, lymphatic vessels are loosely attached together.

Bottom Line: OLAG enables rapid (~seconds) mapping of lymphatic networks, along with blood vessel networks, over 8 mm x 8 mm of human skin and 5 mm x 5 mm of human areola.Moreover, lymphatic system's response to inflammation within human skin is monitored throughout an acne lesion development over 7 days.The demonstrated results promise OLAG as a revolutionary tool in the clinical research and treatment of patients with pathologic conditions such as cancer, diabetes, and autoimmune diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Washington, Seattle, WA, USA.

ABSTRACT
Due to the limitations of current imaging techniques, visualization of lymphatic capillaries within tissue in vivo has been challenging. Here, we present a label-free high resolution optical coherence tomography (OCT) based lymphangiography (OLAG) within human skin in vivo. OLAG enables rapid (~seconds) mapping of lymphatic networks, along with blood vessel networks, over 8 mm x 8 mm of human skin and 5 mm x 5 mm of human areola. Moreover, lymphatic system's response to inflammation within human skin is monitored throughout an acne lesion development over 7 days. The demonstrated results promise OLAG as a revolutionary tool in the clinical research and treatment of patients with pathologic conditions such as cancer, diabetes, and autoimmune diseases.

No MeSH data available.


Related in: MedlinePlus