Limits...
Isolation and partial characterization of a highly divergent lineage of hantavirus from the European mole (Talpa europaea).

Gu SH, Kumar M, Sikorska B, Hejduk J, Markowski J, Markowski M, Liberski PP, Yanagihara R - Sci Rep (2016)

Bottom Line: High NVAV RNA copies were detected in lung, liver, kidney, spleen and brain by quantitative real-time RT-PCR.Neuropathological examination showed astrocytic and microglial activation and neuronal loss.The first mole-borne hantavirus isolate will facilitate long-overdue studies on its infectivity and pathogenic potential in humans.

View Article: PubMed Central - PubMed

Affiliation: Departments of Pediatrics and Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA.

ABSTRACT
Genetically distinct hantaviruses have been identified in five species of fossorial moles (order Eulipotyphla, family Talpidae) from Eurasia and North America. Here, we report the isolation and partial characterization of a highly divergent hantavirus, named Nova virus (NVAV), from lung tissue of a European mole (Talpa europaea), captured in central Poland in August 2013. Typical hantavirus-like particles, measuring 80-120 nm in diameter, were found in NVAV-infected Vero E6 cells by transmission electron microscopy. Whole-genome sequences of the isolate, designated NVAV strain Te34, were identical to that amplified from the original lung tissue, and phylogenetic analysis of the full-length L, M and S segments, using maximum-likelihood and Bayesian methods, showed that NVAV was most closely related to hantaviruses harbored by insectivorous bats, consistent with an ancient evolutionary origin. Infant Swiss Webster mice, inoculated with NVAV by the intraperitoneal route, developed weight loss and hyperactivity, beginning at 16 days, followed by hind-limb paralysis and death. High NVAV RNA copies were detected in lung, liver, kidney, spleen and brain by quantitative real-time RT-PCR. Neuropathological examination showed astrocytic and microglial activation and neuronal loss. The first mole-borne hantavirus isolate will facilitate long-overdue studies on its infectivity and pathogenic potential in humans.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic trees generated by the Bayesian method, using the GTR+I+Γ model of evolution as estimated from the data, based on the alignment of the coding regions of the full- length S, M and L segments of NVAV strain Te34.Topologies of the unrooted phylogenetic trees using the maximum-likelihood method were nearly identical. Relationships are shown to the prototype NVAV strain from Hungary (MSB95703, S: FJ539168; M: HQ840957; L: FJ593498) and to representative NVAV strains from Poland (1135, S: JX990924; L: JX990948; 2086, S: KF515970; L: JX990963; and 2097, S: JX990930; L: KF663727) and France (YA0077, S: KF010573; L: KF010538; YA0087, S: KF010571; L: KF010534; YA0109, S: KF010565; L: KF010521). Other hantaviruses harbored by shrews and moles included Thottapalayam virus (TPMV VRC66412), Imjin virus (MJNV Cl 05-11), Uluguru virus (ULUV FMNH158302), Kilimanjaro virus (KMJV FMNH174124), Asama virus (ASAV N10), Oxbow virus (OXBV Ng1453), Rockport virus (RKPV MSB57412), Jemez Springs virus (JMSV MSB144475), Seewis virus (SWSV mp70), Kenkeme virus (KKMV MSB148794), Qian Hu Shan virus (QHSV YN05-284), Cao Bang virus (CBNV CBN-3), Azagny virus (AZGV KBM15), Tanganya virus (TGNV Tan826), Bowé virus (BOWV VN1512), and Jeju virus (JJUV SH42). Bat-borne hantaviruses included Magboi virus (MGBV MGB1209), Mouyassué virus (MOYV KB576), Huangpi virus (HUPV Pa-1), Longquan virus (LQUV Ra-10), Laibin virus (LBV BT20), and Xuan Son virus (XSV F42682). Also shown are representative rodent-borne hantaviruses, including Hantaan virus (HTNV 76-118), Soochong virus (SOOV SOO-1), Dobrava/Belgrade virus (DOB/BGDV Greece), Seoul virus (SEOV 80-39), Tula virus (TULV M5302v), Puumala virus (PUUV Sotkamo), Prospect Hill virus (PHV PH-1), Sin Nombre virus (SNV NMH10) and Andes virus (ANDV Chile9717869). The numbers at each node are posterior node probabilities based on 150,000 trees. The scale bar indicates nucleotide substitutions per site.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4759689&req=5

f2: Phylogenetic trees generated by the Bayesian method, using the GTR+I+Γ model of evolution as estimated from the data, based on the alignment of the coding regions of the full- length S, M and L segments of NVAV strain Te34.Topologies of the unrooted phylogenetic trees using the maximum-likelihood method were nearly identical. Relationships are shown to the prototype NVAV strain from Hungary (MSB95703, S: FJ539168; M: HQ840957; L: FJ593498) and to representative NVAV strains from Poland (1135, S: JX990924; L: JX990948; 2086, S: KF515970; L: JX990963; and 2097, S: JX990930; L: KF663727) and France (YA0077, S: KF010573; L: KF010538; YA0087, S: KF010571; L: KF010534; YA0109, S: KF010565; L: KF010521). Other hantaviruses harbored by shrews and moles included Thottapalayam virus (TPMV VRC66412), Imjin virus (MJNV Cl 05-11), Uluguru virus (ULUV FMNH158302), Kilimanjaro virus (KMJV FMNH174124), Asama virus (ASAV N10), Oxbow virus (OXBV Ng1453), Rockport virus (RKPV MSB57412), Jemez Springs virus (JMSV MSB144475), Seewis virus (SWSV mp70), Kenkeme virus (KKMV MSB148794), Qian Hu Shan virus (QHSV YN05-284), Cao Bang virus (CBNV CBN-3), Azagny virus (AZGV KBM15), Tanganya virus (TGNV Tan826), Bowé virus (BOWV VN1512), and Jeju virus (JJUV SH42). Bat-borne hantaviruses included Magboi virus (MGBV MGB1209), Mouyassué virus (MOYV KB576), Huangpi virus (HUPV Pa-1), Longquan virus (LQUV Ra-10), Laibin virus (LBV BT20), and Xuan Son virus (XSV F42682). Also shown are representative rodent-borne hantaviruses, including Hantaan virus (HTNV 76-118), Soochong virus (SOOV SOO-1), Dobrava/Belgrade virus (DOB/BGDV Greece), Seoul virus (SEOV 80-39), Tula virus (TULV M5302v), Puumala virus (PUUV Sotkamo), Prospect Hill virus (PHV PH-1), Sin Nombre virus (SNV NMH10) and Andes virus (ANDV Chile9717869). The numbers at each node are posterior node probabilities based on 150,000 trees. The scale bar indicates nucleotide substitutions per site.

Mentions: As determined by maximum-likelihood and Bayesian methods, phylogenetic analysis, based on full-length sequences of each genomic segment, demonstrated tree topologies, well supported by bootstrap analysis and posterior node probabilities, showing the NVAV isolate forming a distinctly divergent clade, which comprised other NVAV strains from Hungary, France and Poland (Fig. 2). Similar topologies were found for deduced amino acid sequences of the encoded proteins (data not shown). Collectively, these data strongly supported an ancient hantavirus-host relationship without evidence of host switching.


Isolation and partial characterization of a highly divergent lineage of hantavirus from the European mole (Talpa europaea).

Gu SH, Kumar M, Sikorska B, Hejduk J, Markowski J, Markowski M, Liberski PP, Yanagihara R - Sci Rep (2016)

Phylogenetic trees generated by the Bayesian method, using the GTR+I+Γ model of evolution as estimated from the data, based on the alignment of the coding regions of the full- length S, M and L segments of NVAV strain Te34.Topologies of the unrooted phylogenetic trees using the maximum-likelihood method were nearly identical. Relationships are shown to the prototype NVAV strain from Hungary (MSB95703, S: FJ539168; M: HQ840957; L: FJ593498) and to representative NVAV strains from Poland (1135, S: JX990924; L: JX990948; 2086, S: KF515970; L: JX990963; and 2097, S: JX990930; L: KF663727) and France (YA0077, S: KF010573; L: KF010538; YA0087, S: KF010571; L: KF010534; YA0109, S: KF010565; L: KF010521). Other hantaviruses harbored by shrews and moles included Thottapalayam virus (TPMV VRC66412), Imjin virus (MJNV Cl 05-11), Uluguru virus (ULUV FMNH158302), Kilimanjaro virus (KMJV FMNH174124), Asama virus (ASAV N10), Oxbow virus (OXBV Ng1453), Rockport virus (RKPV MSB57412), Jemez Springs virus (JMSV MSB144475), Seewis virus (SWSV mp70), Kenkeme virus (KKMV MSB148794), Qian Hu Shan virus (QHSV YN05-284), Cao Bang virus (CBNV CBN-3), Azagny virus (AZGV KBM15), Tanganya virus (TGNV Tan826), Bowé virus (BOWV VN1512), and Jeju virus (JJUV SH42). Bat-borne hantaviruses included Magboi virus (MGBV MGB1209), Mouyassué virus (MOYV KB576), Huangpi virus (HUPV Pa-1), Longquan virus (LQUV Ra-10), Laibin virus (LBV BT20), and Xuan Son virus (XSV F42682). Also shown are representative rodent-borne hantaviruses, including Hantaan virus (HTNV 76-118), Soochong virus (SOOV SOO-1), Dobrava/Belgrade virus (DOB/BGDV Greece), Seoul virus (SEOV 80-39), Tula virus (TULV M5302v), Puumala virus (PUUV Sotkamo), Prospect Hill virus (PHV PH-1), Sin Nombre virus (SNV NMH10) and Andes virus (ANDV Chile9717869). The numbers at each node are posterior node probabilities based on 150,000 trees. The scale bar indicates nucleotide substitutions per site.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4759689&req=5

f2: Phylogenetic trees generated by the Bayesian method, using the GTR+I+Γ model of evolution as estimated from the data, based on the alignment of the coding regions of the full- length S, M and L segments of NVAV strain Te34.Topologies of the unrooted phylogenetic trees using the maximum-likelihood method were nearly identical. Relationships are shown to the prototype NVAV strain from Hungary (MSB95703, S: FJ539168; M: HQ840957; L: FJ593498) and to representative NVAV strains from Poland (1135, S: JX990924; L: JX990948; 2086, S: KF515970; L: JX990963; and 2097, S: JX990930; L: KF663727) and France (YA0077, S: KF010573; L: KF010538; YA0087, S: KF010571; L: KF010534; YA0109, S: KF010565; L: KF010521). Other hantaviruses harbored by shrews and moles included Thottapalayam virus (TPMV VRC66412), Imjin virus (MJNV Cl 05-11), Uluguru virus (ULUV FMNH158302), Kilimanjaro virus (KMJV FMNH174124), Asama virus (ASAV N10), Oxbow virus (OXBV Ng1453), Rockport virus (RKPV MSB57412), Jemez Springs virus (JMSV MSB144475), Seewis virus (SWSV mp70), Kenkeme virus (KKMV MSB148794), Qian Hu Shan virus (QHSV YN05-284), Cao Bang virus (CBNV CBN-3), Azagny virus (AZGV KBM15), Tanganya virus (TGNV Tan826), Bowé virus (BOWV VN1512), and Jeju virus (JJUV SH42). Bat-borne hantaviruses included Magboi virus (MGBV MGB1209), Mouyassué virus (MOYV KB576), Huangpi virus (HUPV Pa-1), Longquan virus (LQUV Ra-10), Laibin virus (LBV BT20), and Xuan Son virus (XSV F42682). Also shown are representative rodent-borne hantaviruses, including Hantaan virus (HTNV 76-118), Soochong virus (SOOV SOO-1), Dobrava/Belgrade virus (DOB/BGDV Greece), Seoul virus (SEOV 80-39), Tula virus (TULV M5302v), Puumala virus (PUUV Sotkamo), Prospect Hill virus (PHV PH-1), Sin Nombre virus (SNV NMH10) and Andes virus (ANDV Chile9717869). The numbers at each node are posterior node probabilities based on 150,000 trees. The scale bar indicates nucleotide substitutions per site.
Mentions: As determined by maximum-likelihood and Bayesian methods, phylogenetic analysis, based on full-length sequences of each genomic segment, demonstrated tree topologies, well supported by bootstrap analysis and posterior node probabilities, showing the NVAV isolate forming a distinctly divergent clade, which comprised other NVAV strains from Hungary, France and Poland (Fig. 2). Similar topologies were found for deduced amino acid sequences of the encoded proteins (data not shown). Collectively, these data strongly supported an ancient hantavirus-host relationship without evidence of host switching.

Bottom Line: High NVAV RNA copies were detected in lung, liver, kidney, spleen and brain by quantitative real-time RT-PCR.Neuropathological examination showed astrocytic and microglial activation and neuronal loss.The first mole-borne hantavirus isolate will facilitate long-overdue studies on its infectivity and pathogenic potential in humans.

View Article: PubMed Central - PubMed

Affiliation: Departments of Pediatrics and Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA.

ABSTRACT
Genetically distinct hantaviruses have been identified in five species of fossorial moles (order Eulipotyphla, family Talpidae) from Eurasia and North America. Here, we report the isolation and partial characterization of a highly divergent hantavirus, named Nova virus (NVAV), from lung tissue of a European mole (Talpa europaea), captured in central Poland in August 2013. Typical hantavirus-like particles, measuring 80-120 nm in diameter, were found in NVAV-infected Vero E6 cells by transmission electron microscopy. Whole-genome sequences of the isolate, designated NVAV strain Te34, were identical to that amplified from the original lung tissue, and phylogenetic analysis of the full-length L, M and S segments, using maximum-likelihood and Bayesian methods, showed that NVAV was most closely related to hantaviruses harbored by insectivorous bats, consistent with an ancient evolutionary origin. Infant Swiss Webster mice, inoculated with NVAV by the intraperitoneal route, developed weight loss and hyperactivity, beginning at 16 days, followed by hind-limb paralysis and death. High NVAV RNA copies were detected in lung, liver, kidney, spleen and brain by quantitative real-time RT-PCR. Neuropathological examination showed astrocytic and microglial activation and neuronal loss. The first mole-borne hantavirus isolate will facilitate long-overdue studies on its infectivity and pathogenic potential in humans.

No MeSH data available.


Related in: MedlinePlus