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Isolation and partial characterization of a highly divergent lineage of hantavirus from the European mole (Talpa europaea).

Gu SH, Kumar M, Sikorska B, Hejduk J, Markowski J, Markowski M, Liberski PP, Yanagihara R - Sci Rep (2016)

Bottom Line: High NVAV RNA copies were detected in lung, liver, kidney, spleen and brain by quantitative real-time RT-PCR.Neuropathological examination showed astrocytic and microglial activation and neuronal loss.The first mole-borne hantavirus isolate will facilitate long-overdue studies on its infectivity and pathogenic potential in humans.

View Article: PubMed Central - PubMed

Affiliation: Departments of Pediatrics and Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA.

ABSTRACT
Genetically distinct hantaviruses have been identified in five species of fossorial moles (order Eulipotyphla, family Talpidae) from Eurasia and North America. Here, we report the isolation and partial characterization of a highly divergent hantavirus, named Nova virus (NVAV), from lung tissue of a European mole (Talpa europaea), captured in central Poland in August 2013. Typical hantavirus-like particles, measuring 80-120 nm in diameter, were found in NVAV-infected Vero E6 cells by transmission electron microscopy. Whole-genome sequences of the isolate, designated NVAV strain Te34, were identical to that amplified from the original lung tissue, and phylogenetic analysis of the full-length L, M and S segments, using maximum-likelihood and Bayesian methods, showed that NVAV was most closely related to hantaviruses harbored by insectivorous bats, consistent with an ancient evolutionary origin. Infant Swiss Webster mice, inoculated with NVAV by the intraperitoneal route, developed weight loss and hyperactivity, beginning at 16 days, followed by hind-limb paralysis and death. High NVAV RNA copies were detected in lung, liver, kidney, spleen and brain by quantitative real-time RT-PCR. Neuropathological examination showed astrocytic and microglial activation and neuronal loss. The first mole-borne hantavirus isolate will facilitate long-overdue studies on its infectivity and pathogenic potential in humans.

No MeSH data available.


Related in: MedlinePlus

(A) European mole (Talpa europaea) captured in Huta Dłutowska, in central Poland, in August 2013. (B) Thin-section transmission electron micrograph, showing hantavirus particles in Vero E6 cells inoculated with lung homogenate from a NVAV-infected European mole. Bar = 100 nm.
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f1: (A) European mole (Talpa europaea) captured in Huta Dłutowska, in central Poland, in August 2013. (B) Thin-section transmission electron micrograph, showing hantavirus particles in Vero E6 cells inoculated with lung homogenate from a NVAV-infected European mole. Bar = 100 nm.

Mentions: Of 22 European moles (Fig. 1A), captured in Huta Dłutowska between June 21, 2013 and August 26, 2013, NVAV RNA was detected by RT-PCR in lung tissue from 11 (50%). Based on the intensity of the RT-PCR amplicon on ethidium bromide-stained gels, lung tissue homogenates were prepared from four European moles and used for virus isolation attempts.


Isolation and partial characterization of a highly divergent lineage of hantavirus from the European mole (Talpa europaea).

Gu SH, Kumar M, Sikorska B, Hejduk J, Markowski J, Markowski M, Liberski PP, Yanagihara R - Sci Rep (2016)

(A) European mole (Talpa europaea) captured in Huta Dłutowska, in central Poland, in August 2013. (B) Thin-section transmission electron micrograph, showing hantavirus particles in Vero E6 cells inoculated with lung homogenate from a NVAV-infected European mole. Bar = 100 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4759689&req=5

f1: (A) European mole (Talpa europaea) captured in Huta Dłutowska, in central Poland, in August 2013. (B) Thin-section transmission electron micrograph, showing hantavirus particles in Vero E6 cells inoculated with lung homogenate from a NVAV-infected European mole. Bar = 100 nm.
Mentions: Of 22 European moles (Fig. 1A), captured in Huta Dłutowska between June 21, 2013 and August 26, 2013, NVAV RNA was detected by RT-PCR in lung tissue from 11 (50%). Based on the intensity of the RT-PCR amplicon on ethidium bromide-stained gels, lung tissue homogenates were prepared from four European moles and used for virus isolation attempts.

Bottom Line: High NVAV RNA copies were detected in lung, liver, kidney, spleen and brain by quantitative real-time RT-PCR.Neuropathological examination showed astrocytic and microglial activation and neuronal loss.The first mole-borne hantavirus isolate will facilitate long-overdue studies on its infectivity and pathogenic potential in humans.

View Article: PubMed Central - PubMed

Affiliation: Departments of Pediatrics and Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA.

ABSTRACT
Genetically distinct hantaviruses have been identified in five species of fossorial moles (order Eulipotyphla, family Talpidae) from Eurasia and North America. Here, we report the isolation and partial characterization of a highly divergent hantavirus, named Nova virus (NVAV), from lung tissue of a European mole (Talpa europaea), captured in central Poland in August 2013. Typical hantavirus-like particles, measuring 80-120 nm in diameter, were found in NVAV-infected Vero E6 cells by transmission electron microscopy. Whole-genome sequences of the isolate, designated NVAV strain Te34, were identical to that amplified from the original lung tissue, and phylogenetic analysis of the full-length L, M and S segments, using maximum-likelihood and Bayesian methods, showed that NVAV was most closely related to hantaviruses harbored by insectivorous bats, consistent with an ancient evolutionary origin. Infant Swiss Webster mice, inoculated with NVAV by the intraperitoneal route, developed weight loss and hyperactivity, beginning at 16 days, followed by hind-limb paralysis and death. High NVAV RNA copies were detected in lung, liver, kidney, spleen and brain by quantitative real-time RT-PCR. Neuropathological examination showed astrocytic and microglial activation and neuronal loss. The first mole-borne hantavirus isolate will facilitate long-overdue studies on its infectivity and pathogenic potential in humans.

No MeSH data available.


Related in: MedlinePlus