Increased Bacterial Load and Expression of Antimicrobial Peptides in Skin of Barrier-Deficient Mice with Reduced Cancer Susceptibility.
Bottom Line: However, reducing the bacterial load by antibiotic treatment or breeding mice under specific pathogen-free conditions did not reduce AMP expression or alleviate the abnormalities in T-cell populations.We conclude that the atopic characteristics of EPI-/- skin are a consequence of the defective barrier rather than a response to the increased bacterial load.It is therefore unlikely that the increase in skin microbiota contributes directly to the observed cancer resistance.
Affiliation: Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge, United Kingdom; Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.Show MeSH
Related in: MedlinePlus
Mentions: To determine whether the increased bacterial load influenced the atopic phenotype of EPI-/- mice, we used two strategies to reduce the bacterial content of the skin. One was to breed EPI-/- mice for multiple generations under specific-pathogen-free (SPF) barrier conditions (flora-deficient EPI-/- mice). The other was to treat them systemically for 2 weeks with the broad-spectrum antibiotic enrofloxacin, a time frame chosen on the basis that the exaggerated atopic response to TPA we described previously (Cipolat et al., 2014) is induced within 9 days. Both treatments reduced the bacterial load of EPI-/- skin to that of WT control mice (Figure 3a).
Affiliation: Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge, United Kingdom; Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.