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Diversity and antagonistic potential of marine microbes collected from south-west coast of India

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ABSTRACT

The diversity of some of the culturable microorganisms associated with marine flora and fauna collected off Vizhinjam and Mulloor coast of South India was evaluated and their bioactive production potential determined. From a total of 24 bacteria, 4 actinomycetes and 8 fungi isolated from diverse marine sources, five bacterial species-BLM3, BSP2, BCS1, BCS4 and BMA6 showed inhibitory activity against at least one of the tested pathogens viz., Klebsiella pneumonia KU1, Pseudomonas aeruginosa VL3, Salmonella enterica typhimurium MTCC 98, Escherichia coli MTCC 40, Micrococcus luteus MTCC 105, Staphylococcus simulans MTCC 3610, Proteus vulgaris MTCC 426, Vibrio fluvialis, Vibrio sp. P3a and Vibrio sp. P3b. The isolated actinomycetes and fungi did not produce significant inhibition zones against the tested pathogens; however, the macroalgal isolated actinomycetes strain AMA1 produced reddish pigment in Starch Casein medium which remained stable till the stationary phase of growth. The marine sediment isolate BCS4, identified as Bacillus sp. showed wide spectrum of activity against the tested Gram positive bacteria, S. simulans MTCC 3610 and Gram negative bacteria, Proteus vulgaris with zone of inhibitions of 25 and 11 mm respectively. Better extraction of the bioactive compound was obtained with ethyl acetate when compared with methanol, benzene and hexane and TLC analysis revealed the presence of an active compound. The 16SrRNA sequencing confirmed the potent strain belong to Bacillus sp. and hence designated Bacillus sp. BCS4.

Electronic supplementary material: The online version of this article (doi:10.1007/s13205-015-0318-1) contains supplementary material, which is available to authorized users.

No MeSH data available.


Growth characteristics andbioactive compound production of Marine bacterial isolate BCS4
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Fig3: Growth characteristics andbioactive compound production of Marine bacterial isolate BCS4

Mentions: The determination of growth of the potent marine isolate Bacillus sp. BCS4 revealed that the growth initiated after about 8 h of incubation, entered into the exponential phase after 24 h. The bacterium showed a steady growth till the 96th h after which it ceased to grow and entered the stationary phase followed by the decline. The steady rise in the growth rate indicated that the production of the metabolites maximized around 48 to 72 h (Fig. 3). The growth was followed by the production of bioactive metabolite which maximized at the 72th h of incubation and remained stable till the 96th h and decreased thereafter.Fig. 3


Diversity and antagonistic potential of marine microbes collected from south-west coast of India
Growth characteristics andbioactive compound production of Marine bacterial isolate BCS4
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697912&req=5

Fig3: Growth characteristics andbioactive compound production of Marine bacterial isolate BCS4
Mentions: The determination of growth of the potent marine isolate Bacillus sp. BCS4 revealed that the growth initiated after about 8 h of incubation, entered into the exponential phase after 24 h. The bacterium showed a steady growth till the 96th h after which it ceased to grow and entered the stationary phase followed by the decline. The steady rise in the growth rate indicated that the production of the metabolites maximized around 48 to 72 h (Fig. 3). The growth was followed by the production of bioactive metabolite which maximized at the 72th h of incubation and remained stable till the 96th h and decreased thereafter.Fig. 3

View Article: PubMed Central - PubMed

ABSTRACT

The diversity of some of the culturable microorganisms associated with marine flora and fauna collected off Vizhinjam and Mulloor coast of South India was evaluated and their bioactive production potential determined. From a total of 24 bacteria, 4 actinomycetes and 8 fungi isolated from diverse marine sources, five bacterial species-BLM3, BSP2, BCS1, BCS4 and BMA6 showed inhibitory activity against at least one of the tested pathogens viz., Klebsiella pneumonia KU1, Pseudomonas aeruginosa VL3, Salmonella enterica typhimurium MTCC 98, Escherichia coli MTCC 40, Micrococcus luteus MTCC 105, Staphylococcus simulans MTCC 3610, Proteus vulgaris MTCC 426, Vibrio fluvialis, Vibrio sp. P3a and Vibrio sp. P3b. The isolated actinomycetes and fungi did not produce significant inhibition zones against the tested pathogens; however, the macroalgal isolated actinomycetes strain AMA1 produced reddish pigment in Starch Casein medium which remained stable till the stationary phase of growth. The marine sediment isolate BCS4, identified as Bacillus sp. showed wide spectrum of activity against the tested Gram positive bacteria, S. simulans MTCC 3610 and Gram negative bacteria, Proteus vulgaris with zone of inhibitions of 25 and 11 mm respectively. Better extraction of the bioactive compound was obtained with ethyl acetate when compared with methanol, benzene and hexane and TLC analysis revealed the presence of an active compound. The 16SrRNA sequencing confirmed the potent strain belong to Bacillus sp. and hence designated Bacillus sp. BCS4.

Electronic supplementary material: The online version of this article (doi:10.1007/s13205-015-0318-1) contains supplementary material, which is available to authorized users.

No MeSH data available.