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Diversity and antagonistic potential of marine microbes collected from south-west coast of India

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ABSTRACT

The diversity of some of the culturable microorganisms associated with marine flora and fauna collected off Vizhinjam and Mulloor coast of South India was evaluated and their bioactive production potential determined. From a total of 24 bacteria, 4 actinomycetes and 8 fungi isolated from diverse marine sources, five bacterial species-BLM3, BSP2, BCS1, BCS4 and BMA6 showed inhibitory activity against at least one of the tested pathogens viz., Klebsiella pneumonia KU1, Pseudomonas aeruginosa VL3, Salmonella enterica typhimurium MTCC 98, Escherichia coli MTCC 40, Micrococcus luteus MTCC 105, Staphylococcus simulans MTCC 3610, Proteus vulgaris MTCC 426, Vibrio fluvialis, Vibrio sp. P3a and Vibrio sp. P3b. The isolated actinomycetes and fungi did not produce significant inhibition zones against the tested pathogens; however, the macroalgal isolated actinomycetes strain AMA1 produced reddish pigment in Starch Casein medium which remained stable till the stationary phase of growth. The marine sediment isolate BCS4, identified as Bacillus sp. showed wide spectrum of activity against the tested Gram positive bacteria, S. simulans MTCC 3610 and Gram negative bacteria, Proteus vulgaris with zone of inhibitions of 25 and 11 mm respectively. Better extraction of the bioactive compound was obtained with ethyl acetate when compared with methanol, benzene and hexane and TLC analysis revealed the presence of an active compound. The 16SrRNA sequencing confirmed the potent strain belong to Bacillus sp. and hence designated Bacillus sp. BCS4.

Electronic supplementary material: The online version of this article (doi:10.1007/s13205-015-0318-1) contains supplementary material, which is available to authorized users.

No MeSH data available.


Marine microbes associated with samples collected off south-west Coast of India
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Fig1: Marine microbes associated with samples collected off south-west Coast of India

Mentions: The samples from the marine environment (Vizhinjam and Mulloor Coast) used for the isolation of marine bacteria, fungi and actinomycetes include the coastal sediments, marine invertebrates viz., crab, sepia and limpet and the marine macroalgae. The isolated microorganisms from the respective agar plates after spread plating and incubation for 24 h were streaked to get pure cultures of the same. The bacteria were grown in Zobell Marine Agar medium; the list and source of the isolated bacterial strains are presented in Table 1. The total count of bacteria per ml of the sample viz., coastal sediments, crab, sepia, limpets and the marine macroalgae was 6.5 × 106, 3.2 × 105, 5.9 × 105, 1.9 × 105 and 2.8 × 104 CFU respectively (Fig. 1).Fig. 1


Diversity and antagonistic potential of marine microbes collected from south-west coast of India
Marine microbes associated with samples collected off south-west Coast of India
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697912&req=5

Fig1: Marine microbes associated with samples collected off south-west Coast of India
Mentions: The samples from the marine environment (Vizhinjam and Mulloor Coast) used for the isolation of marine bacteria, fungi and actinomycetes include the coastal sediments, marine invertebrates viz., crab, sepia and limpet and the marine macroalgae. The isolated microorganisms from the respective agar plates after spread plating and incubation for 24 h were streaked to get pure cultures of the same. The bacteria were grown in Zobell Marine Agar medium; the list and source of the isolated bacterial strains are presented in Table 1. The total count of bacteria per ml of the sample viz., coastal sediments, crab, sepia, limpets and the marine macroalgae was 6.5 × 106, 3.2 × 105, 5.9 × 105, 1.9 × 105 and 2.8 × 104 CFU respectively (Fig. 1).Fig. 1

View Article: PubMed Central - PubMed

ABSTRACT

The diversity of some of the culturable microorganisms associated with marine flora and fauna collected off Vizhinjam and Mulloor coast of South India was evaluated and their bioactive production potential determined. From a total of 24 bacteria, 4 actinomycetes and 8 fungi isolated from diverse marine sources, five bacterial species-BLM3, BSP2, BCS1, BCS4 and BMA6 showed inhibitory activity against at least one of the tested pathogens viz., Klebsiella pneumonia KU1, Pseudomonas aeruginosa VL3, Salmonella enterica typhimurium MTCC 98, Escherichia coli MTCC 40, Micrococcus luteus MTCC 105, Staphylococcus simulans MTCC 3610, Proteus vulgaris MTCC 426, Vibrio fluvialis, Vibrio sp. P3a and Vibrio sp. P3b. The isolated actinomycetes and fungi did not produce significant inhibition zones against the tested pathogens; however, the macroalgal isolated actinomycetes strain AMA1 produced reddish pigment in Starch Casein medium which remained stable till the stationary phase of growth. The marine sediment isolate BCS4, identified as Bacillus sp. showed wide spectrum of activity against the tested Gram positive bacteria, S. simulans MTCC 3610 and Gram negative bacteria, Proteus vulgaris with zone of inhibitions of 25 and 11 mm respectively. Better extraction of the bioactive compound was obtained with ethyl acetate when compared with methanol, benzene and hexane and TLC analysis revealed the presence of an active compound. The 16SrRNA sequencing confirmed the potent strain belong to Bacillus sp. and hence designated Bacillus sp. BCS4.

Electronic supplementary material: The online version of this article (doi:10.1007/s13205-015-0318-1) contains supplementary material, which is available to authorized users.

No MeSH data available.