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Stem Cells in Aggregate Form to Enhance Chondrogenesis in Hydrogels.

Sridharan B, Lin SM, Hwu AT, Laflin AD, Detamore MS - PLoS ONE (2015)

Bottom Line: Despite being differing cell sources, both rBMSC and hWJC aggregates were consistent in outperforming cell suspension control groups in biosynthesis and chondrogenesis.Higher cell density impacted biosynthesis favorably, and the number of aggregates positively influenced chondrogenesis.Therefore, we recommend that investigators employing hydrogels consider using cells in an aggregate form for enhanced chondrogenic performance.

View Article: PubMed Central - PubMed

Affiliation: Bioengineering Graduate Program, University of Kansas, Lawrence, Kansas, United States of America.

ABSTRACT
There are a variety of exciting hydrogel technologies being explored for cartilage regenerative medicine. Our overall goal is to explore whether using stem cells in an aggregate form may be advantageous in these applications. 3D stem cell aggregates hold great promise as they may recapitulate the in vivo skeletal tissue condensation, a property that is not typically observed in 2D culture. We considered two different stem cell sources, human umbilical cord Wharton's jelly cells (hWJCs, currently being used in clinical trials) and rat bone marrow-derived mesenchymal stem cells (rBMSCs). The objective of the current study was to compare the influence of cell phenotype, aggregate size, and aggregate number on chondrogenic differentiation in a generic hydrogel (agarose) platform. Despite being differing cell sources, both rBMSC and hWJC aggregates were consistent in outperforming cell suspension control groups in biosynthesis and chondrogenesis. Higher cell density impacted biosynthesis favorably, and the number of aggregates positively influenced chondrogenesis. Therefore, we recommend that investigators employing hydrogels consider using cells in an aggregate form for enhanced chondrogenic performance.

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Related in: MedlinePlus

Representative images for immunohistochemistry analysis for collagen I, collagen II, and aggrecan staining for rBMSC groups at weeks 0, 2, and 3.At week 2, 20M LA had the most intense staining for collagen II and aggrecan. At week 3, 10M LA had the highest staining intensity at collagen II. Scale bar = 200 μm.
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pone.0141479.g009: Representative images for immunohistochemistry analysis for collagen I, collagen II, and aggrecan staining for rBMSC groups at weeks 0, 2, and 3.At week 2, 20M LA had the most intense staining for collagen II and aggrecan. At week 3, 10M LA had the highest staining intensity at collagen II. Scale bar = 200 μm.

Mentions: IHC staining of the rBMSC aggregates revealed that the diameter of the aggregates decreased over the 3-week period, specifically the 10M HA (Fig 9) and 20M LA aggregates. In contrast, the 20M HA aggregates increased in size from week 0 to week 3. Except for the 10M HA group that showed increased collagen I staining at week 2 compared to week 0, collagen I staining did not increase with time for any other group. Collagen II and aggrecan staining were more intense in the 20M group for LA (especially at 2 weeks), but more intense in the 10M group for HA (especially at 3 weeks).


Stem Cells in Aggregate Form to Enhance Chondrogenesis in Hydrogels.

Sridharan B, Lin SM, Hwu AT, Laflin AD, Detamore MS - PLoS ONE (2015)

Representative images for immunohistochemistry analysis for collagen I, collagen II, and aggrecan staining for rBMSC groups at weeks 0, 2, and 3.At week 2, 20M LA had the most intense staining for collagen II and aggrecan. At week 3, 10M LA had the highest staining intensity at collagen II. Scale bar = 200 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697858&req=5

pone.0141479.g009: Representative images for immunohistochemistry analysis for collagen I, collagen II, and aggrecan staining for rBMSC groups at weeks 0, 2, and 3.At week 2, 20M LA had the most intense staining for collagen II and aggrecan. At week 3, 10M LA had the highest staining intensity at collagen II. Scale bar = 200 μm.
Mentions: IHC staining of the rBMSC aggregates revealed that the diameter of the aggregates decreased over the 3-week period, specifically the 10M HA (Fig 9) and 20M LA aggregates. In contrast, the 20M HA aggregates increased in size from week 0 to week 3. Except for the 10M HA group that showed increased collagen I staining at week 2 compared to week 0, collagen I staining did not increase with time for any other group. Collagen II and aggrecan staining were more intense in the 20M group for LA (especially at 2 weeks), but more intense in the 10M group for HA (especially at 3 weeks).

Bottom Line: Despite being differing cell sources, both rBMSC and hWJC aggregates were consistent in outperforming cell suspension control groups in biosynthesis and chondrogenesis.Higher cell density impacted biosynthesis favorably, and the number of aggregates positively influenced chondrogenesis.Therefore, we recommend that investigators employing hydrogels consider using cells in an aggregate form for enhanced chondrogenic performance.

View Article: PubMed Central - PubMed

Affiliation: Bioengineering Graduate Program, University of Kansas, Lawrence, Kansas, United States of America.

ABSTRACT
There are a variety of exciting hydrogel technologies being explored for cartilage regenerative medicine. Our overall goal is to explore whether using stem cells in an aggregate form may be advantageous in these applications. 3D stem cell aggregates hold great promise as they may recapitulate the in vivo skeletal tissue condensation, a property that is not typically observed in 2D culture. We considered two different stem cell sources, human umbilical cord Wharton's jelly cells (hWJCs, currently being used in clinical trials) and rat bone marrow-derived mesenchymal stem cells (rBMSCs). The objective of the current study was to compare the influence of cell phenotype, aggregate size, and aggregate number on chondrogenic differentiation in a generic hydrogel (agarose) platform. Despite being differing cell sources, both rBMSC and hWJC aggregates were consistent in outperforming cell suspension control groups in biosynthesis and chondrogenesis. Higher cell density impacted biosynthesis favorably, and the number of aggregates positively influenced chondrogenesis. Therefore, we recommend that investigators employing hydrogels consider using cells in an aggregate form for enhanced chondrogenic performance.

Show MeSH
Related in: MedlinePlus