Limits...
Identification of Differentially Expressed Genes Associated with Apple Fruit Ripening and Softening by Suppression Subtractive Hybridization.

Zhang Z, Jiang S, Wang N, Li M, Ji X, Sun S, Liu J, Wang D, Xu H, Qi S, Wu S, Fei Z, Feng S, Chen X - PLoS ONE (2015)

Bottom Line: These genes were involved in cell wall modification, anthocyanin biosynthesis, aroma production, stress response, metabolism, transcription, or were non-annotated.Some genes associated with cell wall modification, anthocyanin biosynthesis and aroma production were up-regulated and significantly correlated with ethylene production, suggesting that fruit texture, coloration and aroma may be regulated by ethylene in 'Taishanzaoxia'.The results contribute to an improved characterization of changes in gene expression during apple fruit ripening and softening.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China.

ABSTRACT
Apple is one of the most economically important horticultural fruit crops worldwide. It is critical to gain insights into fruit ripening and softening to improve apple fruit quality and extend shelf life. In this study, forward and reverse suppression subtractive hybridization libraries were generated from 'Taishanzaoxia' apple fruits sampled around the ethylene climacteric to isolate ripening- and softening-related genes. A set of 648 unigenes were derived from sequence alignment and cluster assembly of 918 expressed sequence tags. According to gene ontology functional classification, 390 out of 443 unigenes (88%) were assigned to the biological process category, 356 unigenes (80%) were classified in the molecular function category, and 381 unigenes (86%) were allocated to the cellular component category. A total of 26 unigenes differentially expressed during fruit development period were analyzed by quantitative RT-PCR. These genes were involved in cell wall modification, anthocyanin biosynthesis, aroma production, stress response, metabolism, transcription, or were non-annotated. Some genes associated with cell wall modification, anthocyanin biosynthesis and aroma production were up-regulated and significantly correlated with ethylene production, suggesting that fruit texture, coloration and aroma may be regulated by ethylene in 'Taishanzaoxia'. Some of the identified unigenes associated with fruit ripening and softening have not been characterized in public databases. The results contribute to an improved characterization of changes in gene expression during apple fruit ripening and softening.

Show MeSH

Related in: MedlinePlus

Fruit firmness and ethylene production in apple ‘Taishanzaoxia’ during the fruit development period.(A) Ethylene production rate, (B) Fruit firmness, (C) Samples used for SSH construction.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4697835&req=5

pone.0146061.g001: Fruit firmness and ethylene production in apple ‘Taishanzaoxia’ during the fruit development period.(A) Ethylene production rate, (B) Fruit firmness, (C) Samples used for SSH construction.

Mentions: Ethylene production and fruit firmness were measured during fruit development period to determine sampling time points for construction of SSH libraries. Ethylene production continued to increase during fruit development period while fruit firmness declined continuously (Fig 1). The ethylene biosynthesis burst at 60–70 DAFB accompanied a dramatic decline in fruit firmness. Important gene expression and physiological changes were predicted to occur during this period. Therefore, samples at 60 DAFB and 70 DAFB were used for construction of SSH libraries to identify differentially expressed genes involved in fruit ripening and softening. The subtraction efficiency of Actin in two libraries was tested by PCR application, and it was as high as 210-fold, suggesting the subtraction was efficient (S2 Fig). The total number of clones in the forward and reverse libraries was 2600 and 2400, respectively.


Identification of Differentially Expressed Genes Associated with Apple Fruit Ripening and Softening by Suppression Subtractive Hybridization.

Zhang Z, Jiang S, Wang N, Li M, Ji X, Sun S, Liu J, Wang D, Xu H, Qi S, Wu S, Fei Z, Feng S, Chen X - PLoS ONE (2015)

Fruit firmness and ethylene production in apple ‘Taishanzaoxia’ during the fruit development period.(A) Ethylene production rate, (B) Fruit firmness, (C) Samples used for SSH construction.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697835&req=5

pone.0146061.g001: Fruit firmness and ethylene production in apple ‘Taishanzaoxia’ during the fruit development period.(A) Ethylene production rate, (B) Fruit firmness, (C) Samples used for SSH construction.
Mentions: Ethylene production and fruit firmness were measured during fruit development period to determine sampling time points for construction of SSH libraries. Ethylene production continued to increase during fruit development period while fruit firmness declined continuously (Fig 1). The ethylene biosynthesis burst at 60–70 DAFB accompanied a dramatic decline in fruit firmness. Important gene expression and physiological changes were predicted to occur during this period. Therefore, samples at 60 DAFB and 70 DAFB were used for construction of SSH libraries to identify differentially expressed genes involved in fruit ripening and softening. The subtraction efficiency of Actin in two libraries was tested by PCR application, and it was as high as 210-fold, suggesting the subtraction was efficient (S2 Fig). The total number of clones in the forward and reverse libraries was 2600 and 2400, respectively.

Bottom Line: These genes were involved in cell wall modification, anthocyanin biosynthesis, aroma production, stress response, metabolism, transcription, or were non-annotated.Some genes associated with cell wall modification, anthocyanin biosynthesis and aroma production were up-regulated and significantly correlated with ethylene production, suggesting that fruit texture, coloration and aroma may be regulated by ethylene in 'Taishanzaoxia'.The results contribute to an improved characterization of changes in gene expression during apple fruit ripening and softening.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China.

ABSTRACT
Apple is one of the most economically important horticultural fruit crops worldwide. It is critical to gain insights into fruit ripening and softening to improve apple fruit quality and extend shelf life. In this study, forward and reverse suppression subtractive hybridization libraries were generated from 'Taishanzaoxia' apple fruits sampled around the ethylene climacteric to isolate ripening- and softening-related genes. A set of 648 unigenes were derived from sequence alignment and cluster assembly of 918 expressed sequence tags. According to gene ontology functional classification, 390 out of 443 unigenes (88%) were assigned to the biological process category, 356 unigenes (80%) were classified in the molecular function category, and 381 unigenes (86%) were allocated to the cellular component category. A total of 26 unigenes differentially expressed during fruit development period were analyzed by quantitative RT-PCR. These genes were involved in cell wall modification, anthocyanin biosynthesis, aroma production, stress response, metabolism, transcription, or were non-annotated. Some genes associated with cell wall modification, anthocyanin biosynthesis and aroma production were up-regulated and significantly correlated with ethylene production, suggesting that fruit texture, coloration and aroma may be regulated by ethylene in 'Taishanzaoxia'. Some of the identified unigenes associated with fruit ripening and softening have not been characterized in public databases. The results contribute to an improved characterization of changes in gene expression during apple fruit ripening and softening.

Show MeSH
Related in: MedlinePlus