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Antidepressants modulate glycine action in rat hippocampus.

Chang HK, Kim KH, Kang KW, Kang YJ, Kim TW, Park HK, Kim SE, Kim CJ - J Exerc Rehabil (2015)

Bottom Line: In the present study, the effects of fluoxetine, tianeptine, and milnacipran on the glycine-induced ion current by nystatin-perforated patch clamp and on the amplitude of field potential in the hippocampal CA1 region by multichannel extracellular recording, MED64, system, were studied.In the present results, fluoxetine, tianeptine, and milnacipran reduced glycine-induced ion current in the hippocampal CA1 neurons in nystatin-perforated patch clamp method.These results suggest that antidepressants may increase neuronal activity by enhancing field potential through inhibition on glycine-induced ion current.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Gachon University Gil Medical Center, Gachon University School of Medicine, Incheon, Korea.

ABSTRACT
Antidepressants are drugs that relieve symptoms of depressive disorders. Fluoxetine, tianeptine, and milnacipran are different types of antidepressants, and they have widely been used for relieving of depression symptoms. In the present study, the effects of fluoxetine, tianeptine, and milnacipran on the glycine-induced ion current by nystatin-perforated patch clamp and on the amplitude of field potential in the hippocampal CA1 region by multichannel extracellular recording, MED64, system, were studied. In the present results, fluoxetine, tianeptine, and milnacipran reduced glycine-induced ion current in the hippocampal CA1 neurons in nystatin-perforated patch clamp method. These drugs enhanced the amplitude of the field potential in the hippocampal CA1 region in MED64 system. These results suggest that antidepressants may increase neuronal activity by enhancing field potential through inhibition on glycine-induced ion current.

No MeSH data available.


Related in: MedlinePlus

Modulation of milnacipran on glycine-activated ion current. Glycine-induced ion current was significantly inhibited by 10−5 M, and 10−4 M of milnacipran. *P<0.05 compared to the control.
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f3-jer-11-6-311: Modulation of milnacipran on glycine-activated ion current. Glycine-induced ion current was significantly inhibited by 10−5 M, and 10−4 M of milnacipran. *P<0.05 compared to the control.

Mentions: To investigate the modulation of milnacipran on the glycine-induced ion current, the magnitude of ion current elicited by 10−5 M glycine was used as the control value and 10−7 M, 10−6 M, 10−5 M, and 10−4 M milnacipran were applied simultaneously with 10−5 M glycine. Milnacipran at concentrations of 10−7 M, 10−6 M, 10−5 M, and 10−4 M suppressed glycine-induced ion current about 0.99%±0.08%, 0.86%±0.03%, 0.81%±0.04%, and 0.67%±0.05% of the control value set as 1. In the present study, the glycine-induced ion current in the hippocampal CA1 neurons was suppressed by application of milnacipran (Fig. 3).


Antidepressants modulate glycine action in rat hippocampus.

Chang HK, Kim KH, Kang KW, Kang YJ, Kim TW, Park HK, Kim SE, Kim CJ - J Exerc Rehabil (2015)

Modulation of milnacipran on glycine-activated ion current. Glycine-induced ion current was significantly inhibited by 10−5 M, and 10−4 M of milnacipran. *P<0.05 compared to the control.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697779&req=5

f3-jer-11-6-311: Modulation of milnacipran on glycine-activated ion current. Glycine-induced ion current was significantly inhibited by 10−5 M, and 10−4 M of milnacipran. *P<0.05 compared to the control.
Mentions: To investigate the modulation of milnacipran on the glycine-induced ion current, the magnitude of ion current elicited by 10−5 M glycine was used as the control value and 10−7 M, 10−6 M, 10−5 M, and 10−4 M milnacipran were applied simultaneously with 10−5 M glycine. Milnacipran at concentrations of 10−7 M, 10−6 M, 10−5 M, and 10−4 M suppressed glycine-induced ion current about 0.99%±0.08%, 0.86%±0.03%, 0.81%±0.04%, and 0.67%±0.05% of the control value set as 1. In the present study, the glycine-induced ion current in the hippocampal CA1 neurons was suppressed by application of milnacipran (Fig. 3).

Bottom Line: In the present study, the effects of fluoxetine, tianeptine, and milnacipran on the glycine-induced ion current by nystatin-perforated patch clamp and on the amplitude of field potential in the hippocampal CA1 region by multichannel extracellular recording, MED64, system, were studied.In the present results, fluoxetine, tianeptine, and milnacipran reduced glycine-induced ion current in the hippocampal CA1 neurons in nystatin-perforated patch clamp method.These results suggest that antidepressants may increase neuronal activity by enhancing field potential through inhibition on glycine-induced ion current.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Gachon University Gil Medical Center, Gachon University School of Medicine, Incheon, Korea.

ABSTRACT
Antidepressants are drugs that relieve symptoms of depressive disorders. Fluoxetine, tianeptine, and milnacipran are different types of antidepressants, and they have widely been used for relieving of depression symptoms. In the present study, the effects of fluoxetine, tianeptine, and milnacipran on the glycine-induced ion current by nystatin-perforated patch clamp and on the amplitude of field potential in the hippocampal CA1 region by multichannel extracellular recording, MED64, system, were studied. In the present results, fluoxetine, tianeptine, and milnacipran reduced glycine-induced ion current in the hippocampal CA1 neurons in nystatin-perforated patch clamp method. These drugs enhanced the amplitude of the field potential in the hippocampal CA1 region in MED64 system. These results suggest that antidepressants may increase neuronal activity by enhancing field potential through inhibition on glycine-induced ion current.

No MeSH data available.


Related in: MedlinePlus