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Epidemiological study of erythromycin-resistant Streptococcus pyogenes from Korea and Japan by emm genotyping and multilocus sequence typing.

Takahashi T, Arai K, Lee DH, Koh EH, Yoshida H, Yano H, Kaku M, Kim S - Ann Lab Med (2016)

Bottom Line: ST36 isolates harbored either erm(B) (56.3%) or mef(A) (37.5%), whereas isolates ST28, ST49, and ST38 carried only mef(A).The proportion of erm(B) and mef(A) was 66.1% and 33.3% in Korea and 22.7% and 68.2% in Japan, respectively.The common STs in Korea and Japan were ST36 and ST49, whereas ST52 was present only in Korea and ST28 only in Japan.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Infectious Diseases, Kitasato Institute for Life Sciences & Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.

ABSTRACT

Background: We determined the epidemiological characteristics of erythromycin (EM)-resistant Streptococcus pyogenes (group A streptococci, GAS) strains isolated from Korea and Japan, using emm genotyping and multilocus sequence typing (MLST).

Methods: Clinical isolates of GAS had been collected from 1992 to 2012 in Korea and from 2004 to 2009 in Japan. EM resistance was determined by the microdilution method, and resistance genotypes were assessed by PCR. The emm genotyping and MLST were performed by DNA sequencing.

Results: The emm genotypes and sequence types (STs) were concordant in 143 (85.1%) of 168 EM-resistant GAS strains from Korea. ST36/emm12 (35.1%), ST52/emm28 (22.6%), and ST49/emm75 (16.1%) were the most common types. Most of the ST36 (93.9%) and ST52 (95.8%) strains harbored erm(B), whereas strains ST49, ST42, and ST15 contained mef(A). The concordance between emm genotypes and STs was 41 (93.2%) among 44 EM-resistant GAS strains from Japan. ST36/emm12 (34.1%), ST49/emm75 (18.2%), and ST28/emm1 (15.9%) were the major types. ST36 isolates harbored either erm(B) (56.3%) or mef(A) (37.5%), whereas isolates ST28, ST49, and ST38 carried only mef(A). The proportion of erm(B) and mef(A) was 66.1% and 33.3% in Korea and 22.7% and 68.2% in Japan, respectively.

Conclusions: The common STs in Korea and Japan were ST36 and ST49, whereas ST52 was present only in Korea and ST28 only in Japan. Genotype erm(B) was predominant in Korea, whereas mef(A) was frequent in Japan. There were differences between Korea and Japan regarding the frequencies of emm genotypes, STs, and EM resistance genes among the EM-resistant GAS.

No MeSH data available.


Prevalence of sequence types (STs) by different periods in Korea (A) and in Japan (B).
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Figure 1: Prevalence of sequence types (STs) by different periods in Korea (A) and in Japan (B).

Mentions: The prevalence rates of STs of the EM-resistant isolates from Korea and Japan are shown in Fig. 1A and B, respectively. In 2002, ST36 was predominant (46.8%) in Korea, whereas ST52 was rare (1.1%). However, the prevalence of ST36 declined (7.1%) and that of ST52 increased remarkably (71.4%) during 2006-2009. ST49 was observed only in 2002. The distribution of the STs between 2002 and 2006-2009 in Korea was significantly different (P=0.0000). In Japan, ST36 was predominant in both periods of 2004-2007 and 2008-2009.


Epidemiological study of erythromycin-resistant Streptococcus pyogenes from Korea and Japan by emm genotyping and multilocus sequence typing.

Takahashi T, Arai K, Lee DH, Koh EH, Yoshida H, Yano H, Kaku M, Kim S - Ann Lab Med (2016)

Prevalence of sequence types (STs) by different periods in Korea (A) and in Japan (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697353&req=5

Figure 1: Prevalence of sequence types (STs) by different periods in Korea (A) and in Japan (B).
Mentions: The prevalence rates of STs of the EM-resistant isolates from Korea and Japan are shown in Fig. 1A and B, respectively. In 2002, ST36 was predominant (46.8%) in Korea, whereas ST52 was rare (1.1%). However, the prevalence of ST36 declined (7.1%) and that of ST52 increased remarkably (71.4%) during 2006-2009. ST49 was observed only in 2002. The distribution of the STs between 2002 and 2006-2009 in Korea was significantly different (P=0.0000). In Japan, ST36 was predominant in both periods of 2004-2007 and 2008-2009.

Bottom Line: ST36 isolates harbored either erm(B) (56.3%) or mef(A) (37.5%), whereas isolates ST28, ST49, and ST38 carried only mef(A).The proportion of erm(B) and mef(A) was 66.1% and 33.3% in Korea and 22.7% and 68.2% in Japan, respectively.The common STs in Korea and Japan were ST36 and ST49, whereas ST52 was present only in Korea and ST28 only in Japan.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Infectious Diseases, Kitasato Institute for Life Sciences & Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.

ABSTRACT

Background: We determined the epidemiological characteristics of erythromycin (EM)-resistant Streptococcus pyogenes (group A streptococci, GAS) strains isolated from Korea and Japan, using emm genotyping and multilocus sequence typing (MLST).

Methods: Clinical isolates of GAS had been collected from 1992 to 2012 in Korea and from 2004 to 2009 in Japan. EM resistance was determined by the microdilution method, and resistance genotypes were assessed by PCR. The emm genotyping and MLST were performed by DNA sequencing.

Results: The emm genotypes and sequence types (STs) were concordant in 143 (85.1%) of 168 EM-resistant GAS strains from Korea. ST36/emm12 (35.1%), ST52/emm28 (22.6%), and ST49/emm75 (16.1%) were the most common types. Most of the ST36 (93.9%) and ST52 (95.8%) strains harbored erm(B), whereas strains ST49, ST42, and ST15 contained mef(A). The concordance between emm genotypes and STs was 41 (93.2%) among 44 EM-resistant GAS strains from Japan. ST36/emm12 (34.1%), ST49/emm75 (18.2%), and ST28/emm1 (15.9%) were the major types. ST36 isolates harbored either erm(B) (56.3%) or mef(A) (37.5%), whereas isolates ST28, ST49, and ST38 carried only mef(A). The proportion of erm(B) and mef(A) was 66.1% and 33.3% in Korea and 22.7% and 68.2% in Japan, respectively.

Conclusions: The common STs in Korea and Japan were ST36 and ST49, whereas ST52 was present only in Korea and ST28 only in Japan. Genotype erm(B) was predominant in Korea, whereas mef(A) was frequent in Japan. There were differences between Korea and Japan regarding the frequencies of emm genotypes, STs, and EM resistance genes among the EM-resistant GAS.

No MeSH data available.