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WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis.

Bakshi M, Vahabi K, Bhattacharya S, Sherameti I, Varma A, Yeh KW, Baldwin I, Johri AK, Oelmüller R - BMC Plant Biol. (2015)

Bottom Line: The results demonstrate that P. indica promotes phosphate uptake and root development under Pi limitation in wrky6 mutant.This is associated with the stimulation of PHOSPHATE1 expression and ethylene production.They can be tested for optimizing root growth in model and agricultural plants.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Botany and Plant Physiology, Friedrich-Schiller-University Jena, Dornburgerstr. 159, D-07743, Jena, Germany. bakshi.madhunita@gmail.com.

ABSTRACT

Background: Arabidopsis root growth is stimulated by Piriformospora indica, phosphate limitation and inactivation of the WRKY6 transcription factor. Combinations of these factors induce unexpected alterations in root and shoot growth, root architecture and root gene expression profiles.

Results: The results demonstrate that P. indica promotes phosphate uptake and root development under Pi limitation in wrky6 mutant. This is associated with the stimulation of PHOSPHATE1 expression and ethylene production. Expression profiles from the roots of wrky6 seedlings identified genes involved in hormone metabolism, transport, meristem, cell and plastid proliferation, and growth regulation. 25 miRNAs were also up-regulated in these roots. We generated and discuss here a list of common genes which are regulated in growing roots and which are common to all three growth stimuli investigated in this study.

Conclusion: Since root development of wrky6 plants exposed to P. indica under phosphate limitation is strongly promoted, we propose that common genes which respond to all three growth stimuli are central for the control of root growth and architecture. They can be tested for optimizing root growth in model and agricultural plants.

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Related in: MedlinePlus

Common up- and down-regulated genes (>4 fold) in Arabidopsis roots regulated either by Pi limitation, WRKY6 or P. indica (Venn diagram). For experimental details, cf. Methods and Results
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Fig9: Common up- and down-regulated genes (>4 fold) in Arabidopsis roots regulated either by Pi limitation, WRKY6 or P. indica (Venn diagram). For experimental details, cf. Methods and Results

Mentions: The Venn-diagrams (Fig. 9) generated by the MAPMAN software identified genes, which were regulated by LP (but not P. indica or WRKY6), by P. indica (but not LP or WRKY6), and by WRKY6 (but not P. indica or LP). The common genes among these three datasets are not specific for one of the three stimuli and should therefore represent more general genes involved in the promotion of root growth and development (Fig. 9). Those genes were then arranged according to their average fold regulation in all datasets (Table 1, 4-fold; Additional file 2: Table S2). The higher the genes are ranked in the list, the more important appears to be their requirement during root growth.Fig. 9


WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis.

Bakshi M, Vahabi K, Bhattacharya S, Sherameti I, Varma A, Yeh KW, Baldwin I, Johri AK, Oelmüller R - BMC Plant Biol. (2015)

Common up- and down-regulated genes (>4 fold) in Arabidopsis roots regulated either by Pi limitation, WRKY6 or P. indica (Venn diagram). For experimental details, cf. Methods and Results
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4697310&req=5

Fig9: Common up- and down-regulated genes (>4 fold) in Arabidopsis roots regulated either by Pi limitation, WRKY6 or P. indica (Venn diagram). For experimental details, cf. Methods and Results
Mentions: The Venn-diagrams (Fig. 9) generated by the MAPMAN software identified genes, which were regulated by LP (but not P. indica or WRKY6), by P. indica (but not LP or WRKY6), and by WRKY6 (but not P. indica or LP). The common genes among these three datasets are not specific for one of the three stimuli and should therefore represent more general genes involved in the promotion of root growth and development (Fig. 9). Those genes were then arranged according to their average fold regulation in all datasets (Table 1, 4-fold; Additional file 2: Table S2). The higher the genes are ranked in the list, the more important appears to be their requirement during root growth.Fig. 9

Bottom Line: The results demonstrate that P. indica promotes phosphate uptake and root development under Pi limitation in wrky6 mutant.This is associated with the stimulation of PHOSPHATE1 expression and ethylene production.They can be tested for optimizing root growth in model and agricultural plants.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Botany and Plant Physiology, Friedrich-Schiller-University Jena, Dornburgerstr. 159, D-07743, Jena, Germany. bakshi.madhunita@gmail.com.

ABSTRACT

Background: Arabidopsis root growth is stimulated by Piriformospora indica, phosphate limitation and inactivation of the WRKY6 transcription factor. Combinations of these factors induce unexpected alterations in root and shoot growth, root architecture and root gene expression profiles.

Results: The results demonstrate that P. indica promotes phosphate uptake and root development under Pi limitation in wrky6 mutant. This is associated with the stimulation of PHOSPHATE1 expression and ethylene production. Expression profiles from the roots of wrky6 seedlings identified genes involved in hormone metabolism, transport, meristem, cell and plastid proliferation, and growth regulation. 25 miRNAs were also up-regulated in these roots. We generated and discuss here a list of common genes which are regulated in growing roots and which are common to all three growth stimuli investigated in this study.

Conclusion: Since root development of wrky6 plants exposed to P. indica under phosphate limitation is strongly promoted, we propose that common genes which respond to all three growth stimuli are central for the control of root growth and architecture. They can be tested for optimizing root growth in model and agricultural plants.

Show MeSH
Related in: MedlinePlus