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WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis.

Bakshi M, Vahabi K, Bhattacharya S, Sherameti I, Varma A, Yeh KW, Baldwin I, Johri AK, Oelmüller R - BMC Plant Biol. (2015)

Bottom Line: The results demonstrate that P. indica promotes phosphate uptake and root development under Pi limitation in wrky6 mutant.This is associated with the stimulation of PHOSPHATE1 expression and ethylene production.They can be tested for optimizing root growth in model and agricultural plants.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Botany and Plant Physiology, Friedrich-Schiller-University Jena, Dornburgerstr. 159, D-07743, Jena, Germany. bakshi.madhunita@gmail.com.

ABSTRACT

Background: Arabidopsis root growth is stimulated by Piriformospora indica, phosphate limitation and inactivation of the WRKY6 transcription factor. Combinations of these factors induce unexpected alterations in root and shoot growth, root architecture and root gene expression profiles.

Results: The results demonstrate that P. indica promotes phosphate uptake and root development under Pi limitation in wrky6 mutant. This is associated with the stimulation of PHOSPHATE1 expression and ethylene production. Expression profiles from the roots of wrky6 seedlings identified genes involved in hormone metabolism, transport, meristem, cell and plastid proliferation, and growth regulation. 25 miRNAs were also up-regulated in these roots. We generated and discuss here a list of common genes which are regulated in growing roots and which are common to all three growth stimuli investigated in this study.

Conclusion: Since root development of wrky6 plants exposed to P. indica under phosphate limitation is strongly promoted, we propose that common genes which respond to all three growth stimuli are central for the control of root growth and architecture. They can be tested for optimizing root growth in model and agricultural plants.

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Related in: MedlinePlus

PHO1 expression after 3 days of co-cultivation or mock-treatment of the two symbionts under the two different Pi concentrations. Data are based on 3 independent experiments with 15 plants each. Bars represent SEs. Asterisks indicate significant differences, as determined by Student’s paired t-test for two tailed distribution (** P ≤ 0.01; *** P ≤ 0.001)
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Fig6: PHO1 expression after 3 days of co-cultivation or mock-treatment of the two symbionts under the two different Pi concentrations. Data are based on 3 independent experiments with 15 plants each. Bars represent SEs. Asterisks indicate significant differences, as determined by Student’s paired t-test for two tailed distribution (** P ≤ 0.01; *** P ≤ 0.001)

Mentions: PHO1 is a high affinity Pi transporter expressed predominantly in the roots, and the gene is up-regulated under low Pi conditions [39]. We observed a ~3.8-fold stimulation of PHO1 expression by P. indica in wrky6 roots under Pi limitation, but not under NP conditions (Fig. 6). This confirms previous observations that WRKY6 acts as a repressor of PHO1 expression under NP conditions. The strong effect of P. indica on root development is reflected by the up-regulation of PHO1 under Pi limitation.Fig. 6


WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis.

Bakshi M, Vahabi K, Bhattacharya S, Sherameti I, Varma A, Yeh KW, Baldwin I, Johri AK, Oelmüller R - BMC Plant Biol. (2015)

PHO1 expression after 3 days of co-cultivation or mock-treatment of the two symbionts under the two different Pi concentrations. Data are based on 3 independent experiments with 15 plants each. Bars represent SEs. Asterisks indicate significant differences, as determined by Student’s paired t-test for two tailed distribution (** P ≤ 0.01; *** P ≤ 0.001)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4697310&req=5

Fig6: PHO1 expression after 3 days of co-cultivation or mock-treatment of the two symbionts under the two different Pi concentrations. Data are based on 3 independent experiments with 15 plants each. Bars represent SEs. Asterisks indicate significant differences, as determined by Student’s paired t-test for two tailed distribution (** P ≤ 0.01; *** P ≤ 0.001)
Mentions: PHO1 is a high affinity Pi transporter expressed predominantly in the roots, and the gene is up-regulated under low Pi conditions [39]. We observed a ~3.8-fold stimulation of PHO1 expression by P. indica in wrky6 roots under Pi limitation, but not under NP conditions (Fig. 6). This confirms previous observations that WRKY6 acts as a repressor of PHO1 expression under NP conditions. The strong effect of P. indica on root development is reflected by the up-regulation of PHO1 under Pi limitation.Fig. 6

Bottom Line: The results demonstrate that P. indica promotes phosphate uptake and root development under Pi limitation in wrky6 mutant.This is associated with the stimulation of PHOSPHATE1 expression and ethylene production.They can be tested for optimizing root growth in model and agricultural plants.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Botany and Plant Physiology, Friedrich-Schiller-University Jena, Dornburgerstr. 159, D-07743, Jena, Germany. bakshi.madhunita@gmail.com.

ABSTRACT

Background: Arabidopsis root growth is stimulated by Piriformospora indica, phosphate limitation and inactivation of the WRKY6 transcription factor. Combinations of these factors induce unexpected alterations in root and shoot growth, root architecture and root gene expression profiles.

Results: The results demonstrate that P. indica promotes phosphate uptake and root development under Pi limitation in wrky6 mutant. This is associated with the stimulation of PHOSPHATE1 expression and ethylene production. Expression profiles from the roots of wrky6 seedlings identified genes involved in hormone metabolism, transport, meristem, cell and plastid proliferation, and growth regulation. 25 miRNAs were also up-regulated in these roots. We generated and discuss here a list of common genes which are regulated in growing roots and which are common to all three growth stimuli investigated in this study.

Conclusion: Since root development of wrky6 plants exposed to P. indica under phosphate limitation is strongly promoted, we propose that common genes which respond to all three growth stimuli are central for the control of root growth and architecture. They can be tested for optimizing root growth in model and agricultural plants.

Show MeSH
Related in: MedlinePlus