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WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis.

Bakshi M, Vahabi K, Bhattacharya S, Sherameti I, Varma A, Yeh KW, Baldwin I, Johri AK, Oelmüller R - BMC Plant Biol. (2015)

Bottom Line: The results demonstrate that P. indica promotes phosphate uptake and root development under Pi limitation in wrky6 mutant.This is associated with the stimulation of PHOSPHATE1 expression and ethylene production.They can be tested for optimizing root growth in model and agricultural plants.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Botany and Plant Physiology, Friedrich-Schiller-University Jena, Dornburgerstr. 159, D-07743, Jena, Germany. bakshi.madhunita@gmail.com.

ABSTRACT

Background: Arabidopsis root growth is stimulated by Piriformospora indica, phosphate limitation and inactivation of the WRKY6 transcription factor. Combinations of these factors induce unexpected alterations in root and shoot growth, root architecture and root gene expression profiles.

Results: The results demonstrate that P. indica promotes phosphate uptake and root development under Pi limitation in wrky6 mutant. This is associated with the stimulation of PHOSPHATE1 expression and ethylene production. Expression profiles from the roots of wrky6 seedlings identified genes involved in hormone metabolism, transport, meristem, cell and plastid proliferation, and growth regulation. 25 miRNAs were also up-regulated in these roots. We generated and discuss here a list of common genes which are regulated in growing roots and which are common to all three growth stimuli investigated in this study.

Conclusion: Since root development of wrky6 plants exposed to P. indica under phosphate limitation is strongly promoted, we propose that common genes which respond to all three growth stimuli are central for the control of root growth and architecture. They can be tested for optimizing root growth in model and agricultural plants.

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Related in: MedlinePlus

a Colonization of WT and wrky6 Arabidopsis roots by P. indica after co-cultivation under 0.25 mM and 2.5 mM Pi concentrations in the media. The fungal material was stained with Trypan Blue. Photos are representative for more than 10 repetitions. b Quantification of the degree of root colonization by quantitative RT-PCR. The amount of fungal material (determined as fungal DNA with P. indica-specific primers) is expressed relative to the plant GAPDH DNA (determined with GAPDH-specific primers). Graphs are based on 3 independent experiments with 20 plants each. Bars represent SEs. Asterisk indicates significant difference, as determined by Student’s paired t-test for two tailed distribution (* P ≤ 0.05)
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Fig4: a Colonization of WT and wrky6 Arabidopsis roots by P. indica after co-cultivation under 0.25 mM and 2.5 mM Pi concentrations in the media. The fungal material was stained with Trypan Blue. Photos are representative for more than 10 repetitions. b Quantification of the degree of root colonization by quantitative RT-PCR. The amount of fungal material (determined as fungal DNA with P. indica-specific primers) is expressed relative to the plant GAPDH DNA (determined with GAPDH-specific primers). Graphs are based on 3 independent experiments with 20 plants each. Bars represent SEs. Asterisk indicates significant difference, as determined by Student’s paired t-test for two tailed distribution (* P ≤ 0.05)

Mentions: To assess the effect of Pi on root colonization, the fungal spores associated with Arabidopsis roots were stained with Trypan Blue. Fig. 4a demonstrates that the number of spores associated with WT roots was higher under LP than NP conditions, and this was not observed for wrky6 seedlings. The colonization by P. indica was also confirmed by quantitative RT-PCR with the P. indica-specific marker gene EF- H, relative to the plant GAPDH gene (Fig. 4b). These results highlight the strong effect of root colonization for WT seedlings grown under LP conditions (cf. Discussion).Fig. 4


WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis.

Bakshi M, Vahabi K, Bhattacharya S, Sherameti I, Varma A, Yeh KW, Baldwin I, Johri AK, Oelmüller R - BMC Plant Biol. (2015)

a Colonization of WT and wrky6 Arabidopsis roots by P. indica after co-cultivation under 0.25 mM and 2.5 mM Pi concentrations in the media. The fungal material was stained with Trypan Blue. Photos are representative for more than 10 repetitions. b Quantification of the degree of root colonization by quantitative RT-PCR. The amount of fungal material (determined as fungal DNA with P. indica-specific primers) is expressed relative to the plant GAPDH DNA (determined with GAPDH-specific primers). Graphs are based on 3 independent experiments with 20 plants each. Bars represent SEs. Asterisk indicates significant difference, as determined by Student’s paired t-test for two tailed distribution (* P ≤ 0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4697310&req=5

Fig4: a Colonization of WT and wrky6 Arabidopsis roots by P. indica after co-cultivation under 0.25 mM and 2.5 mM Pi concentrations in the media. The fungal material was stained with Trypan Blue. Photos are representative for more than 10 repetitions. b Quantification of the degree of root colonization by quantitative RT-PCR. The amount of fungal material (determined as fungal DNA with P. indica-specific primers) is expressed relative to the plant GAPDH DNA (determined with GAPDH-specific primers). Graphs are based on 3 independent experiments with 20 plants each. Bars represent SEs. Asterisk indicates significant difference, as determined by Student’s paired t-test for two tailed distribution (* P ≤ 0.05)
Mentions: To assess the effect of Pi on root colonization, the fungal spores associated with Arabidopsis roots were stained with Trypan Blue. Fig. 4a demonstrates that the number of spores associated with WT roots was higher under LP than NP conditions, and this was not observed for wrky6 seedlings. The colonization by P. indica was also confirmed by quantitative RT-PCR with the P. indica-specific marker gene EF- H, relative to the plant GAPDH gene (Fig. 4b). These results highlight the strong effect of root colonization for WT seedlings grown under LP conditions (cf. Discussion).Fig. 4

Bottom Line: The results demonstrate that P. indica promotes phosphate uptake and root development under Pi limitation in wrky6 mutant.This is associated with the stimulation of PHOSPHATE1 expression and ethylene production.They can be tested for optimizing root growth in model and agricultural plants.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Botany and Plant Physiology, Friedrich-Schiller-University Jena, Dornburgerstr. 159, D-07743, Jena, Germany. bakshi.madhunita@gmail.com.

ABSTRACT

Background: Arabidopsis root growth is stimulated by Piriformospora indica, phosphate limitation and inactivation of the WRKY6 transcription factor. Combinations of these factors induce unexpected alterations in root and shoot growth, root architecture and root gene expression profiles.

Results: The results demonstrate that P. indica promotes phosphate uptake and root development under Pi limitation in wrky6 mutant. This is associated with the stimulation of PHOSPHATE1 expression and ethylene production. Expression profiles from the roots of wrky6 seedlings identified genes involved in hormone metabolism, transport, meristem, cell and plastid proliferation, and growth regulation. 25 miRNAs were also up-regulated in these roots. We generated and discuss here a list of common genes which are regulated in growing roots and which are common to all three growth stimuli investigated in this study.

Conclusion: Since root development of wrky6 plants exposed to P. indica under phosphate limitation is strongly promoted, we propose that common genes which respond to all three growth stimuli are central for the control of root growth and architecture. They can be tested for optimizing root growth in model and agricultural plants.

Show MeSH
Related in: MedlinePlus