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Effect of 50% enantiomeric excess bupivacaine mixture combined with pancuronium on neuromuscular transmission in rat phrenic nerve-diaphragm preparation; a pilot study.

de Assunção Braga Ade F, Carvalho VH, da Silva Braga FS, Potério GM, Santos FN, Junqueira FE - Indian J Anaesth (2015)

Bottom Line: However, the effects of these drugs on the neuromuscular junction and their influence on the blockade produced by non-depolarising neuromuscular blocking drugs are still under investigation.A 50% enantiomeric excess bupivacaine mixture did not alter the amplitude of muscle response (MP) but decreased the frequency and amplitude of MEPP.No action was shown on the muscle fibre, and alterations on MEPPs demonstrated a presynaptic action.

View Article: PubMed Central - PubMed

Affiliation: Department of Anaesthesiology, School of Medicine, UNICAMP, Campinas, São Paulo, Brazil.

ABSTRACT

Background and aims: Local anaesthetics are drugs that are widely used in clinical practice. However, the effects of these drugs on the neuromuscular junction and their influence on the blockade produced by non-depolarising neuromuscular blocking drugs are still under investigation. The aim of this study was to evaluate, in vitro, the influence of a 50% enantiomeric excess bupivacaine mixture on neuromuscular transmission and neuromuscular block produced by pancuronium.

Methods: Rats were distributed into three groups (n = 5) according to the drug studied namely, 50% enantiomeric excess bupivacaine mixture (5 μg/mL); pancuronium (2 μg/mL); 50% enantiomeric excess bupivacaine mixture + pancuronium. The following parameters were evaluated: (1) Effects of a 50% enantiomeric excess bupivacaine mixture on membrane potential (MP) and miniature endplate potentials (MEPPs); (2) amplitude of diaphragmatic response before and 60 min after the addition of a 50% enantiomeric excess bupivacaine mixture; the degree of neuromuscular block with pancuronium and pancuronium combined with a 50% enantiomeric excess bupivacaine mixture.

Results: A 50% enantiomeric excess bupivacaine mixture did not alter the amplitude of muscle response (MP) but decreased the frequency and amplitude of MEPP. The block produced by pancuronium was potentiated by a 50% enantiomeric excess bupivacaine mixture.

Conclusion: A 50% enantiomeric excess bupivacaine mixture used alone did not affect neuromuscular transmission, but potentiated the neuromuscular block produced by pancuronium. No action was shown on the muscle fibre, and alterations on MEPPs demonstrated a presynaptic action.

No MeSH data available.


Related in: MedlinePlus

Degree of blockade with R25-S75 and pancuronium alone and in preparation previously exposed to R25-S75
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Figure 1: Degree of blockade with R25-S75 and pancuronium alone and in preparation previously exposed to R25-S75

Mentions: In the concentration studied, R25-S75% did not cause any reduction in the amplitude of muscle response to indirect stimulation, in a rat phrenic nerve-diaphragm preparation when used as the sole drug. Blockade produced by pancuronium alone was (54.9 ± 14.1%). In preparations previously exposed to R25-S75%, pancuronium produced a 100% block, with a significant difference (P = 0.015) compared to the block produced by pancuronium alone [Figure 1]. No significant effect of R25-S75% on MPs was observed [Figure 2]. The effects on MEPPs (PPTM) were characterised by a decrease in the frequency and amplitude until complete block [Figure 3].


Effect of 50% enantiomeric excess bupivacaine mixture combined with pancuronium on neuromuscular transmission in rat phrenic nerve-diaphragm preparation; a pilot study.

de Assunção Braga Ade F, Carvalho VH, da Silva Braga FS, Potério GM, Santos FN, Junqueira FE - Indian J Anaesth (2015)

Degree of blockade with R25-S75 and pancuronium alone and in preparation previously exposed to R25-S75
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697241&req=5

Figure 1: Degree of blockade with R25-S75 and pancuronium alone and in preparation previously exposed to R25-S75
Mentions: In the concentration studied, R25-S75% did not cause any reduction in the amplitude of muscle response to indirect stimulation, in a rat phrenic nerve-diaphragm preparation when used as the sole drug. Blockade produced by pancuronium alone was (54.9 ± 14.1%). In preparations previously exposed to R25-S75%, pancuronium produced a 100% block, with a significant difference (P = 0.015) compared to the block produced by pancuronium alone [Figure 1]. No significant effect of R25-S75% on MPs was observed [Figure 2]. The effects on MEPPs (PPTM) were characterised by a decrease in the frequency and amplitude until complete block [Figure 3].

Bottom Line: However, the effects of these drugs on the neuromuscular junction and their influence on the blockade produced by non-depolarising neuromuscular blocking drugs are still under investigation.A 50% enantiomeric excess bupivacaine mixture did not alter the amplitude of muscle response (MP) but decreased the frequency and amplitude of MEPP.No action was shown on the muscle fibre, and alterations on MEPPs demonstrated a presynaptic action.

View Article: PubMed Central - PubMed

Affiliation: Department of Anaesthesiology, School of Medicine, UNICAMP, Campinas, São Paulo, Brazil.

ABSTRACT

Background and aims: Local anaesthetics are drugs that are widely used in clinical practice. However, the effects of these drugs on the neuromuscular junction and their influence on the blockade produced by non-depolarising neuromuscular blocking drugs are still under investigation. The aim of this study was to evaluate, in vitro, the influence of a 50% enantiomeric excess bupivacaine mixture on neuromuscular transmission and neuromuscular block produced by pancuronium.

Methods: Rats were distributed into three groups (n = 5) according to the drug studied namely, 50% enantiomeric excess bupivacaine mixture (5 μg/mL); pancuronium (2 μg/mL); 50% enantiomeric excess bupivacaine mixture + pancuronium. The following parameters were evaluated: (1) Effects of a 50% enantiomeric excess bupivacaine mixture on membrane potential (MP) and miniature endplate potentials (MEPPs); (2) amplitude of diaphragmatic response before and 60 min after the addition of a 50% enantiomeric excess bupivacaine mixture; the degree of neuromuscular block with pancuronium and pancuronium combined with a 50% enantiomeric excess bupivacaine mixture.

Results: A 50% enantiomeric excess bupivacaine mixture did not alter the amplitude of muscle response (MP) but decreased the frequency and amplitude of MEPP. The block produced by pancuronium was potentiated by a 50% enantiomeric excess bupivacaine mixture.

Conclusion: A 50% enantiomeric excess bupivacaine mixture used alone did not affect neuromuscular transmission, but potentiated the neuromuscular block produced by pancuronium. No action was shown on the muscle fibre, and alterations on MEPPs demonstrated a presynaptic action.

No MeSH data available.


Related in: MedlinePlus