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Antioxidative and antidiabetic activities of watermelon (Citrullus lanatus) juice on oxidative stress in alloxan-induced diabetic male Wistar albino rats.

Oseni OA, Odesanmi OE, Oladele FC - Niger Med J (2015 Jul-Aug)

Bottom Line: The results of the biochemical analyses showed a significant increase in the concentration of blood glucose level after treatment with alloxan, which indicates that diabetic was induced.Hence, watermelon juice caused increased in weight, hypoglycemia; and increases in GSH, GPx, catalase, and SOD % inhibition activities with reduced MDA concentration after treatments.The watermelon juice resulted in the restoration of impaired conditions of the rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Ekiti-State University, Ado-Ekiti, Nigeria.

ABSTRACT

Background: The nutritional and medicinal importance of watermelon has been emphasized and its diseases preventive and curative power must be evaluated. Hence, this study was designed to evaluate the antioxidative and antidiabetic potentials of watermelon.

Materials and methods: The in vivo assay was carried out on 15 male albino rats which were divided into groups of three stages. In stage I, all animals received normal feeds and water for 1-week after, which five animals were selected and sacrificed for biochemical analyses which form the nondiabetic control, group. The remaining animals were fasted for 24 h before injected intra-peritoneally with a freshly prepared solution of alloxan at a dosage of 35 mg/kg body weight. Five out of the 10 rats were sacrificed as diabetic group while last five animals were fed with water melon juice for a week after, which they were sacrificed to form the treated group animals. In all the groups, body weights, fasting blood sugar, total protein level in the blood, and other biochemical parameters such as reduced glutathione (GSH), glutathione peroxidase (GPx), malondialdehyde (MDA) concentration; catalase, and superoxide dismutase (SOD) % inhibition activities were determined.

Results: The results of the biochemical analyses showed a significant increase in the concentration of blood glucose level after treatment with alloxan, which indicates that diabetic was induced. Hence, watermelon juice caused increased in weight, hypoglycemia; and increases in GSH, GPx, catalase, and SOD % inhibition activities with reduced MDA concentration after treatments.

Conclusion: The watermelon juice resulted in the restoration of impaired conditions of the rats.

No MeSH data available.


Related in: MedlinePlus

Effects of watermelon (Citrullus lanatus) juice treatment on the catalase activity of alloxan-induced diabetic rat
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Figure 6: Effects of watermelon (Citrullus lanatus) juice treatment on the catalase activity of alloxan-induced diabetic rat

Mentions: Figure 6 revealed the effects of watermelon (C. lanatus) juice treatment on the catalase activity of alloxan-induced diabetic rat. The reduction in catalase activity after injection with alloxan is another significant finding in this study. The decreased concentration of plasma catalase is attributable in part to the reduced synthesis of this antioxidant enzyme (which functions in the detoxification of hydrogen perioxide) whose concentrations would have fallen with the alloxan that was injected into the animals. Though some studies have reported no alterations in the activity of red cell catalase in diabetics.26 However, this study is in agreement with earlier reports by Ochuko et al., Udoh et al., and Tagami et al.212728 who reported a decreased in red blood cell catalase activity in a diabetic. While treatment with watermelon (C. lanatus) juice caused a slight increase in catalase activity in their respective groups.


Antioxidative and antidiabetic activities of watermelon (Citrullus lanatus) juice on oxidative stress in alloxan-induced diabetic male Wistar albino rats.

Oseni OA, Odesanmi OE, Oladele FC - Niger Med J (2015 Jul-Aug)

Effects of watermelon (Citrullus lanatus) juice treatment on the catalase activity of alloxan-induced diabetic rat
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697216&req=5

Figure 6: Effects of watermelon (Citrullus lanatus) juice treatment on the catalase activity of alloxan-induced diabetic rat
Mentions: Figure 6 revealed the effects of watermelon (C. lanatus) juice treatment on the catalase activity of alloxan-induced diabetic rat. The reduction in catalase activity after injection with alloxan is another significant finding in this study. The decreased concentration of plasma catalase is attributable in part to the reduced synthesis of this antioxidant enzyme (which functions in the detoxification of hydrogen perioxide) whose concentrations would have fallen with the alloxan that was injected into the animals. Though some studies have reported no alterations in the activity of red cell catalase in diabetics.26 However, this study is in agreement with earlier reports by Ochuko et al., Udoh et al., and Tagami et al.212728 who reported a decreased in red blood cell catalase activity in a diabetic. While treatment with watermelon (C. lanatus) juice caused a slight increase in catalase activity in their respective groups.

Bottom Line: The results of the biochemical analyses showed a significant increase in the concentration of blood glucose level after treatment with alloxan, which indicates that diabetic was induced.Hence, watermelon juice caused increased in weight, hypoglycemia; and increases in GSH, GPx, catalase, and SOD % inhibition activities with reduced MDA concentration after treatments.The watermelon juice resulted in the restoration of impaired conditions of the rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Ekiti-State University, Ado-Ekiti, Nigeria.

ABSTRACT

Background: The nutritional and medicinal importance of watermelon has been emphasized and its diseases preventive and curative power must be evaluated. Hence, this study was designed to evaluate the antioxidative and antidiabetic potentials of watermelon.

Materials and methods: The in vivo assay was carried out on 15 male albino rats which were divided into groups of three stages. In stage I, all animals received normal feeds and water for 1-week after, which five animals were selected and sacrificed for biochemical analyses which form the nondiabetic control, group. The remaining animals were fasted for 24 h before injected intra-peritoneally with a freshly prepared solution of alloxan at a dosage of 35 mg/kg body weight. Five out of the 10 rats were sacrificed as diabetic group while last five animals were fed with water melon juice for a week after, which they were sacrificed to form the treated group animals. In all the groups, body weights, fasting blood sugar, total protein level in the blood, and other biochemical parameters such as reduced glutathione (GSH), glutathione peroxidase (GPx), malondialdehyde (MDA) concentration; catalase, and superoxide dismutase (SOD) % inhibition activities were determined.

Results: The results of the biochemical analyses showed a significant increase in the concentration of blood glucose level after treatment with alloxan, which indicates that diabetic was induced. Hence, watermelon juice caused increased in weight, hypoglycemia; and increases in GSH, GPx, catalase, and SOD % inhibition activities with reduced MDA concentration after treatments.

Conclusion: The watermelon juice resulted in the restoration of impaired conditions of the rats.

No MeSH data available.


Related in: MedlinePlus