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Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response.

Wang D, Qin Y, Han J, Zhang L, Xu X, Liu X, Wang C, Liu X - Biotechnol. Biotechnol. Equip. (2014)

Bottom Line: Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response.It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines.The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

View Article: PubMed Central - PubMed

Affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.

ABSTRACT

Rice blast resistance (R) genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes pi21 in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene OsWRKY45 of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, JAmyb (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) R genes Pib, Pizt, Pik, Pita2 and Pikahei than in NB and KHS. SalT of abscisic acid (ABA) signalling may be involved in the R/Avr interaction, including Pizt, Pik, pi21 and Pikahei. However, SalT was shown to negatively regulate Pib/AvrPib interaction. OsPR1b and PBZ1 were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response. It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

No MeSH data available.


Expression pattern of OsSGT1 in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
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f0007: Expression pattern of OsSGT1 in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.

Mentions: Our results showed that OsRAR1 expression levels were downregulated in all of the tested lines, except for NB-Pita2 and KHR (Figure 6). In addition, OsSGT1 was upregulated in five lines (NB-Pib, NB-Pizt, NB-Pik, NB-Pita2 and KHR) to 2-, 2-, 2-, 2.5- and 4.5-fold of their background by 24 h p.i. (Figure 7). They subsequently decreased rapidly by 48 h p.i in NB, NB-Pib, NB-Pizt, NB-Pik and NB-Pita2. OsSGT1 was upregulated in two lines: AA and AA-pi21. The expression levels were 1.6-fold at 24 h in AA and 2.6-fold at 48 h in AA-pi21, demonstrating a substantial increase in the resistant line AA-pi21 (Figure 7). OsSGT1 was induced at 24 h in two lines (KHR and KHS), where the expression level of KHR was much higher than in KHS.Figure 6.


Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response.

Wang D, Qin Y, Han J, Zhang L, Xu X, Liu X, Wang C, Liu X - Biotechnol. Biotechnol. Equip. (2014)

Expression pattern of OsSGT1 in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697195&req=5

f0007: Expression pattern of OsSGT1 in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
Mentions: Our results showed that OsRAR1 expression levels were downregulated in all of the tested lines, except for NB-Pita2 and KHR (Figure 6). In addition, OsSGT1 was upregulated in five lines (NB-Pib, NB-Pizt, NB-Pik, NB-Pita2 and KHR) to 2-, 2-, 2-, 2.5- and 4.5-fold of their background by 24 h p.i. (Figure 7). They subsequently decreased rapidly by 48 h p.i in NB, NB-Pib, NB-Pizt, NB-Pik and NB-Pita2. OsSGT1 was upregulated in two lines: AA and AA-pi21. The expression levels were 1.6-fold at 24 h in AA and 2.6-fold at 48 h in AA-pi21, demonstrating a substantial increase in the resistant line AA-pi21 (Figure 7). OsSGT1 was induced at 24 h in two lines (KHR and KHS), where the expression level of KHR was much higher than in KHS.Figure 6.

Bottom Line: Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response.It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines.The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

View Article: PubMed Central - PubMed

Affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.

ABSTRACT

Rice blast resistance (R) genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes pi21 in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene OsWRKY45 of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, JAmyb (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) R genes Pib, Pizt, Pik, Pita2 and Pikahei than in NB and KHS. SalT of abscisic acid (ABA) signalling may be involved in the R/Avr interaction, including Pizt, Pik, pi21 and Pikahei. However, SalT was shown to negatively regulate Pib/AvrPib interaction. OsPR1b and PBZ1 were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response. It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

No MeSH data available.