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Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response.

Wang D, Qin Y, Han J, Zhang L, Xu X, Liu X, Wang C, Liu X - Biotechnol. Biotechnol. Equip. (2014)

Bottom Line: Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response.It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines.The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

View Article: PubMed Central - PubMed

Affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.

ABSTRACT

Rice blast resistance (R) genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes pi21 in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene OsWRKY45 of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, JAmyb (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) R genes Pib, Pizt, Pik, Pita2 and Pikahei than in NB and KHS. SalT of abscisic acid (ABA) signalling may be involved in the R/Avr interaction, including Pizt, Pik, pi21 and Pikahei. However, SalT was shown to negatively regulate Pib/AvrPib interaction. OsPR1b and PBZ1 were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response. It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

No MeSH data available.


Expression pattern of OsPR1b in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
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f0004: Expression pattern of OsPR1b in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.

Mentions: In our study, at the tested time point, OsPR1b was shown to be upregulated in four lines (NB, NB-Pib, NB-Pizt and NB-Pik). However, much higher expression levels were shown at 48 h p.i. than at 24 h p.i. No expression was observed in NB-Pita2 at the three time points. OsPR1b was significantly induced by 48 h p.i. in AA and AA-pi21; however, the expression level in AA-pi21 was higher than that in AA (Figure 4). Strangely, OsPR1b was not expressed in lines KHS and KHR, and an experimental error was ruled out. PBZ1 was significantly elevated at 24 h in five lines (NB-Pib, NB-Pizt, NB-Pik, NB-Pita2 and AA-pi21), peaking at 48 h at approximately 340-, 155-, 156-, 45- and 35-fold, respectively (Figure 5). Compared with NB, the expression level of PBZ1 in the resistant lines was lower. The expression profiles of PBZ1 in AA and AA-pi21 were similar to that of OsPR1b. However, PBZ1 was upregulated with expression levels in KHS higher than those in KHR (Figure 5).Figure 4.


Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response.

Wang D, Qin Y, Han J, Zhang L, Xu X, Liu X, Wang C, Liu X - Biotechnol. Biotechnol. Equip. (2014)

Expression pattern of OsPR1b in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697195&req=5

f0004: Expression pattern of OsPR1b in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
Mentions: In our study, at the tested time point, OsPR1b was shown to be upregulated in four lines (NB, NB-Pib, NB-Pizt and NB-Pik). However, much higher expression levels were shown at 48 h p.i. than at 24 h p.i. No expression was observed in NB-Pita2 at the three time points. OsPR1b was significantly induced by 48 h p.i. in AA and AA-pi21; however, the expression level in AA-pi21 was higher than that in AA (Figure 4). Strangely, OsPR1b was not expressed in lines KHS and KHR, and an experimental error was ruled out. PBZ1 was significantly elevated at 24 h in five lines (NB-Pib, NB-Pizt, NB-Pik, NB-Pita2 and AA-pi21), peaking at 48 h at approximately 340-, 155-, 156-, 45- and 35-fold, respectively (Figure 5). Compared with NB, the expression level of PBZ1 in the resistant lines was lower. The expression profiles of PBZ1 in AA and AA-pi21 were similar to that of OsPR1b. However, PBZ1 was upregulated with expression levels in KHS higher than those in KHR (Figure 5).Figure 4.

Bottom Line: Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response.It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines.The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

View Article: PubMed Central - PubMed

Affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.

ABSTRACT

Rice blast resistance (R) genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes pi21 in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene OsWRKY45 of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, JAmyb (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) R genes Pib, Pizt, Pik, Pita2 and Pikahei than in NB and KHS. SalT of abscisic acid (ABA) signalling may be involved in the R/Avr interaction, including Pizt, Pik, pi21 and Pikahei. However, SalT was shown to negatively regulate Pib/AvrPib interaction. OsPR1b and PBZ1 were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response. It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

No MeSH data available.