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Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response.

Wang D, Qin Y, Han J, Zhang L, Xu X, Liu X, Wang C, Liu X - Biotechnol. Biotechnol. Equip. (2014)

Bottom Line: Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response.It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines.The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

View Article: PubMed Central - PubMed

Affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.

ABSTRACT

Rice blast resistance (R) genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes pi21 in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene OsWRKY45 of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, JAmyb (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) R genes Pib, Pizt, Pik, Pita2 and Pikahei than in NB and KHS. SalT of abscisic acid (ABA) signalling may be involved in the R/Avr interaction, including Pizt, Pik, pi21 and Pikahei. However, SalT was shown to negatively regulate Pib/AvrPib interaction. OsPR1b and PBZ1 were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response. It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

No MeSH data available.


Expression pattern of SalT in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
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f0003: Expression pattern of SalT in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.

Mentions: ABA has also been identified as a key signalling molecule in plant–pathogen interactions, enhancing the susceptibility of rice to blast fungus through the molecular mechanisms behind ABA.[19] ABA treatment has been shown to suppress SAR induction, indicating an antagonistic interaction between SA and ABA signalling.[20] Our results showed differential expression profiles of SalT, which is a marker gene for the ABA-signalling pathway, in the resistance response. In the true resistance response, SalT was induced and reached a 19-, 12- and 24-fold increase in the expression levels by 24 h p.i. in lines NB, NB-Pizt and NB-Pik, respectively (Figure 3). Only slight upregulation was shown in NB-Pita2 and downregulation in NB-Pib. In the field resistance gene-mediated resistance response, SalT was downregulated in the susceptible lines AA and KHS, significantly upregulated (46.5-fold) in AA-pi21 and upregulated 15-fold in KHR at 24 h p.i. (Figure 3). The expression levels of SalT did not change and were not downregulated in the tested lines by 48 h p.i. These results suggest the involvement of SalT in the R/Avr interaction, including R genes Pizt, Pik, pi21 and Pikahei. Moreover, SalT may have also negatively regulated Pib/AvrPib interaction.Figure 3.


Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response.

Wang D, Qin Y, Han J, Zhang L, Xu X, Liu X, Wang C, Liu X - Biotechnol. Biotechnol. Equip. (2014)

Expression pattern of SalT in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697195&req=5

f0003: Expression pattern of SalT in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
Mentions: ABA has also been identified as a key signalling molecule in plant–pathogen interactions, enhancing the susceptibility of rice to blast fungus through the molecular mechanisms behind ABA.[19] ABA treatment has been shown to suppress SAR induction, indicating an antagonistic interaction between SA and ABA signalling.[20] Our results showed differential expression profiles of SalT, which is a marker gene for the ABA-signalling pathway, in the resistance response. In the true resistance response, SalT was induced and reached a 19-, 12- and 24-fold increase in the expression levels by 24 h p.i. in lines NB, NB-Pizt and NB-Pik, respectively (Figure 3). Only slight upregulation was shown in NB-Pita2 and downregulation in NB-Pib. In the field resistance gene-mediated resistance response, SalT was downregulated in the susceptible lines AA and KHS, significantly upregulated (46.5-fold) in AA-pi21 and upregulated 15-fold in KHR at 24 h p.i. (Figure 3). The expression levels of SalT did not change and were not downregulated in the tested lines by 48 h p.i. These results suggest the involvement of SalT in the R/Avr interaction, including R genes Pizt, Pik, pi21 and Pikahei. Moreover, SalT may have also negatively regulated Pib/AvrPib interaction.Figure 3.

Bottom Line: Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response.It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines.The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

View Article: PubMed Central - PubMed

Affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.

ABSTRACT

Rice blast resistance (R) genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes pi21 in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene OsWRKY45 of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, JAmyb (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) R genes Pib, Pizt, Pik, Pita2 and Pikahei than in NB and KHS. SalT of abscisic acid (ABA) signalling may be involved in the R/Avr interaction, including Pizt, Pik, pi21 and Pikahei. However, SalT was shown to negatively regulate Pib/AvrPib interaction. OsPR1b and PBZ1 were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response. It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

No MeSH data available.