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Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response.

Wang D, Qin Y, Han J, Zhang L, Xu X, Liu X, Wang C, Liu X - Biotechnol. Biotechnol. Equip. (2014)

Bottom Line: Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response.It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines.The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

View Article: PubMed Central - PubMed

Affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.

ABSTRACT

Rice blast resistance (R) genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes pi21 in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene OsWRKY45 of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, JAmyb (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) R genes Pib, Pizt, Pik, Pita2 and Pikahei than in NB and KHS. SalT of abscisic acid (ABA) signalling may be involved in the R/Avr interaction, including Pizt, Pik, pi21 and Pikahei. However, SalT was shown to negatively regulate Pib/AvrPib interaction. OsPR1b and PBZ1 were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response. It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

No MeSH data available.


Expression pattern of JAmyb in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
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f0002: Expression pattern of JAmyb in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.

Mentions: Several studies have suggested that the JA signalling pathway is an essential and important endogenous signal implicated in induced resistance to pathogen infection.[18] In our experiments, the expression patterns of JAmyb in the four resistant NB lines (NB-Pib, NB-Pizt, NB-Pik and NB-Pita2) were not similar. They all showed upregulation, but their expression levels differed significantly, being highest in NB-Pita2 (Figure 2). JAmyb was upregulated at 24 h, but then its expression decreased by 48 h p.i. In NB, the expression levels were upregulated at 24 h followed by a slight rise at 48 h. The expression levels of the four resistance lines were much higher than those shown in NB. JAmyb was induced in two lines with a 62-fold increase at 24 h in AA and a 61-fold increase at 48 h in AA-pi21 (Figure 2). JAmyb was also upregulated in KHS and KHR, peaking at 24 h; however, KHR demonstrated slightly higher expression levels than KHS. That is, JAmyb showed higher upregulation in resistance lines with NBS-LRR R genes (NB-Pib, NB-Pizt, NB-Pik, NB-Pita2 and KHR with Pikahei) than in NB and KHS, suggesting that NBS-LRR R genes may be specifically involved in the JA signalling pathway. Moreover, JAmyb was rapidly upregulated by 24 h p.i., but decreased significantly by 48 h p.i., indicating that JA signalling may play an important role in the early stages of the blast fungus infection by causing a downstream defence response.Figure 2.


Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response.

Wang D, Qin Y, Han J, Zhang L, Xu X, Liu X, Wang C, Liu X - Biotechnol. Biotechnol. Equip. (2014)

Expression pattern of JAmyb in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697195&req=5

f0002: Expression pattern of JAmyb in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
Mentions: Several studies have suggested that the JA signalling pathway is an essential and important endogenous signal implicated in induced resistance to pathogen infection.[18] In our experiments, the expression patterns of JAmyb in the four resistant NB lines (NB-Pib, NB-Pizt, NB-Pik and NB-Pita2) were not similar. They all showed upregulation, but their expression levels differed significantly, being highest in NB-Pita2 (Figure 2). JAmyb was upregulated at 24 h, but then its expression decreased by 48 h p.i. In NB, the expression levels were upregulated at 24 h followed by a slight rise at 48 h. The expression levels of the four resistance lines were much higher than those shown in NB. JAmyb was induced in two lines with a 62-fold increase at 24 h in AA and a 61-fold increase at 48 h in AA-pi21 (Figure 2). JAmyb was also upregulated in KHS and KHR, peaking at 24 h; however, KHR demonstrated slightly higher expression levels than KHS. That is, JAmyb showed higher upregulation in resistance lines with NBS-LRR R genes (NB-Pib, NB-Pizt, NB-Pik, NB-Pita2 and KHR with Pikahei) than in NB and KHS, suggesting that NBS-LRR R genes may be specifically involved in the JA signalling pathway. Moreover, JAmyb was rapidly upregulated by 24 h p.i., but decreased significantly by 48 h p.i., indicating that JA signalling may play an important role in the early stages of the blast fungus infection by causing a downstream defence response.Figure 2.

Bottom Line: Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response.It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines.The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

View Article: PubMed Central - PubMed

Affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.

ABSTRACT

Rice blast resistance (R) genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes pi21 in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene OsWRKY45 of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, JAmyb (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) R genes Pib, Pizt, Pik, Pita2 and Pikahei than in NB and KHS. SalT of abscisic acid (ABA) signalling may be involved in the R/Avr interaction, including Pizt, Pik, pi21 and Pikahei. However, SalT was shown to negatively regulate Pib/AvrPib interaction. OsPR1b and PBZ1 were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response. It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

No MeSH data available.