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Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response.

Wang D, Qin Y, Han J, Zhang L, Xu X, Liu X, Wang C, Liu X - Biotechnol. Biotechnol. Equip. (2014)

Bottom Line: Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response.It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines.The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

View Article: PubMed Central - PubMed

Affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.

ABSTRACT

Rice blast resistance (R) genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes pi21 in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene OsWRKY45 of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, JAmyb (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) R genes Pib, Pizt, Pik, Pita2 and Pikahei than in NB and KHS. SalT of abscisic acid (ABA) signalling may be involved in the R/Avr interaction, including Pizt, Pik, pi21 and Pikahei. However, SalT was shown to negatively regulate Pib/AvrPib interaction. OsPR1b and PBZ1 were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response. It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

No MeSH data available.


Expression pattern of OsWRKY45 in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
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f0001: Expression pattern of OsWRKY45 in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.

Mentions: OsWRKY45 is specifically induced by SA and is regarded as a marker gene involved in the SA signalling pathway in defence responses.[10] In our study, the relative expression of this marker gene was analysed by qRT-PCR. The results showed that the OsWRKY45 gene was induced at 24 h in lines NB, NB-Pib, NB-Pizt, NB-Pik and NB-Pita2. The expression level was shown to increase slightly by 48 h p.i. in the susceptible cultivar NB, which was accompanied by a rapid increase to 15-, 9-, 8- and 16-fold of the background levels in the resistance lines (Figure 1). Further studies with a greater range of time points would be needed to verify these results.Figure 1.


Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response.

Wang D, Qin Y, Han J, Zhang L, Xu X, Liu X, Wang C, Liu X - Biotechnol. Biotechnol. Equip. (2014)

Expression pattern of OsWRKY45 in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697195&req=5

f0001: Expression pattern of OsWRKY45 in the studied rice lines at 0 h, 24 h and 48 h p.i. with M. oryzae spores.
Mentions: OsWRKY45 is specifically induced by SA and is regarded as a marker gene involved in the SA signalling pathway in defence responses.[10] In our study, the relative expression of this marker gene was analysed by qRT-PCR. The results showed that the OsWRKY45 gene was induced at 24 h in lines NB, NB-Pib, NB-Pizt, NB-Pik and NB-Pita2. The expression level was shown to increase slightly by 48 h p.i. in the susceptible cultivar NB, which was accompanied by a rapid increase to 15-, 9-, 8- and 16-fold of the background levels in the resistance lines (Figure 1). Further studies with a greater range of time points would be needed to verify these results.Figure 1.

Bottom Line: Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response.It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines.The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

View Article: PubMed Central - PubMed

Affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.

ABSTRACT

Rice blast resistance (R) genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes pi21 in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene OsWRKY45 of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, JAmyb (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) R genes Pib, Pizt, Pik, Pita2 and Pikahei than in NB and KHS. SalT of abscisic acid (ABA) signalling may be involved in the R/Avr interaction, including Pizt, Pik, pi21 and Pikahei. However, SalT was shown to negatively regulate Pib/AvrPib interaction. OsPR1b and PBZ1 were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response. It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

No MeSH data available.