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A new modified animal model of myosin-induced experimental autoimmune myositis enhanced by defibrase.

Wen-Jing L, Chuan-Qiang P, Hong-Hua L, Xiang-Hui L, Jie-Xiao L - Arch Med Sci (2015)

Bottom Line: Histological analysis revealed a significant difference.Defibrase-treated animals displayed extensive inflammation and fiber necrosis compared with the EAM group (histological score: 2.80 ±1.15 vs. 1.88 ±1.32, p < 0.05).Defibrase can exacerbate myosin-induced EAM; thus a new modified model was generated.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Chinese PLA General Hospital, Beijing, China; Department of Neurology, Chinese PLA Wuhan General Hospital of Guangzhou Military Command, Wuhan, China.

ABSTRACT

Introduction: We investigated the effect of defibrase (a proteolytic enzyme extraction of Agkistrodon halys venom) on experimental autoimmune myositis (EAM) in guinea pigs and explored the option of using a modified pig model of EAM to enhance the study of this disease.

Material and methods: Guinea pigs were divided into 3 groups: group A (control group) was immunized with complete Freund adjuvant (CFA), then received 6 injections of saline weekly; group B (EAM group) was immunized with partially purified rabbit myosin emulsified with CFA, then received an injection of saline; group C (EAM + defibrase group) was immunized with purified rabbit myosin emulsified with CFA, then received an injection of defibrase. The animals were observed for their general health condition and the body weight was measured daily. Plasma levels of fibrinogen and creatine kinase (CK) were determined. Muscle tissues were examined histologically.

Results: After immunizations for 6 weeks, incidence of EAM in groups A, B and C was 0 (0/7), 83.3% (10/12) and 100% (15/15), respectively. Guinea pigs with EAM presented angeitis symptoms of muscle weakness. Histological analysis revealed a significant difference. Muscles with EAM had scattered or diffuse inflammatory manifestations, which are also common pathological features of human idiopathic polymyositis (IPM). Defibrase-treated animals displayed extensive inflammation and fiber necrosis compared with the EAM group (histological score: 2.80 ±1.15 vs. 1.88 ±1.32, p < 0.05). Severity of inflammation of group B was mainly mild to moderate; 16.7% (2/12) of animals developed severe inflammation. Incidence of severe inflammation with a score up to 4 in group C was 40% (6/15).

Conclusions: Defibrase can exacerbate myosin-induced EAM; thus a new modified model was generated.

No MeSH data available.


Related in: MedlinePlus

HE stain result of muscle in 3 groups (100×). A – Group A (normal control), morphology and structure of muscle fibers are normal, no infiltration of inflammatory cells could be observed. B – Group B (EAM), initial inflammation involving a single fiber with infiltration of mononuclear inflammatory cells, particle muscle fiber was degenerated, necrosis could be observed with surrounding infiltrating mononuclear cells. C – Group B, a number of fibers were degenerated and necrotic, surrounded by mononuclear cells
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Figure 0001: HE stain result of muscle in 3 groups (100×). A – Group A (normal control), morphology and structure of muscle fibers are normal, no infiltration of inflammatory cells could be observed. B – Group B (EAM), initial inflammation involving a single fiber with infiltration of mononuclear inflammatory cells, particle muscle fiber was degenerated, necrosis could be observed with surrounding infiltrating mononuclear cells. C – Group B, a number of fibers were degenerated and necrotic, surrounded by mononuclear cells

Mentions: Histological analysis results (Table I) revealed no existence of obvious necrosis or inflammation in group A animals. No animal showed any dystrophy, degeneration or necrosis of muscle fiber, or infiltration of inflammatory cells (Figure 1 A). However, the majority of animals in group B had intensive inflammation and necrosis, and 83.3% of animals suffered EAM confirmed by histological examination. Muscles of EAM displayed mild to severe inflammatory features of degeneration, necrosis and phagocytosis of fibers with infiltration of mononuclear cells in the endomysium and epimysium (Figures 1 B, C). For group C, defibrase induced intensive inflammation and necrosis. The muscle pathology data in 3 groups are collected in Table II. All the animals displayed histological features of myositis. The histological score in group C was the highest among the 3 groups. More severe inflammatory damage was observed in this group (and) 40% (6/15) of animals developed extensive lesions with a score up to 4. We observed that many fibers were necrotic, accompanied with infiltrate of numerous mononuclear cells, macrophages and plasmocytes (Figures 2 A–C). However, the majority of animals in group B (8/12, 66.7%) displayed mild to moderate inflammation with a score of 1–2.5 and only 16.7% (2/12) of animals had intensive inflammation (Table II).


A new modified animal model of myosin-induced experimental autoimmune myositis enhanced by defibrase.

Wen-Jing L, Chuan-Qiang P, Hong-Hua L, Xiang-Hui L, Jie-Xiao L - Arch Med Sci (2015)

HE stain result of muscle in 3 groups (100×). A – Group A (normal control), morphology and structure of muscle fibers are normal, no infiltration of inflammatory cells could be observed. B – Group B (EAM), initial inflammation involving a single fiber with infiltration of mononuclear inflammatory cells, particle muscle fiber was degenerated, necrosis could be observed with surrounding infiltrating mononuclear cells. C – Group B, a number of fibers were degenerated and necrotic, surrounded by mononuclear cells
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697045&req=5

Figure 0001: HE stain result of muscle in 3 groups (100×). A – Group A (normal control), morphology and structure of muscle fibers are normal, no infiltration of inflammatory cells could be observed. B – Group B (EAM), initial inflammation involving a single fiber with infiltration of mononuclear inflammatory cells, particle muscle fiber was degenerated, necrosis could be observed with surrounding infiltrating mononuclear cells. C – Group B, a number of fibers were degenerated and necrotic, surrounded by mononuclear cells
Mentions: Histological analysis results (Table I) revealed no existence of obvious necrosis or inflammation in group A animals. No animal showed any dystrophy, degeneration or necrosis of muscle fiber, or infiltration of inflammatory cells (Figure 1 A). However, the majority of animals in group B had intensive inflammation and necrosis, and 83.3% of animals suffered EAM confirmed by histological examination. Muscles of EAM displayed mild to severe inflammatory features of degeneration, necrosis and phagocytosis of fibers with infiltration of mononuclear cells in the endomysium and epimysium (Figures 1 B, C). For group C, defibrase induced intensive inflammation and necrosis. The muscle pathology data in 3 groups are collected in Table II. All the animals displayed histological features of myositis. The histological score in group C was the highest among the 3 groups. More severe inflammatory damage was observed in this group (and) 40% (6/15) of animals developed extensive lesions with a score up to 4. We observed that many fibers were necrotic, accompanied with infiltrate of numerous mononuclear cells, macrophages and plasmocytes (Figures 2 A–C). However, the majority of animals in group B (8/12, 66.7%) displayed mild to moderate inflammation with a score of 1–2.5 and only 16.7% (2/12) of animals had intensive inflammation (Table II).

Bottom Line: Histological analysis revealed a significant difference.Defibrase-treated animals displayed extensive inflammation and fiber necrosis compared with the EAM group (histological score: 2.80 ±1.15 vs. 1.88 ±1.32, p < 0.05).Defibrase can exacerbate myosin-induced EAM; thus a new modified model was generated.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Chinese PLA General Hospital, Beijing, China; Department of Neurology, Chinese PLA Wuhan General Hospital of Guangzhou Military Command, Wuhan, China.

ABSTRACT

Introduction: We investigated the effect of defibrase (a proteolytic enzyme extraction of Agkistrodon halys venom) on experimental autoimmune myositis (EAM) in guinea pigs and explored the option of using a modified pig model of EAM to enhance the study of this disease.

Material and methods: Guinea pigs were divided into 3 groups: group A (control group) was immunized with complete Freund adjuvant (CFA), then received 6 injections of saline weekly; group B (EAM group) was immunized with partially purified rabbit myosin emulsified with CFA, then received an injection of saline; group C (EAM + defibrase group) was immunized with purified rabbit myosin emulsified with CFA, then received an injection of defibrase. The animals were observed for their general health condition and the body weight was measured daily. Plasma levels of fibrinogen and creatine kinase (CK) were determined. Muscle tissues were examined histologically.

Results: After immunizations for 6 weeks, incidence of EAM in groups A, B and C was 0 (0/7), 83.3% (10/12) and 100% (15/15), respectively. Guinea pigs with EAM presented angeitis symptoms of muscle weakness. Histological analysis revealed a significant difference. Muscles with EAM had scattered or diffuse inflammatory manifestations, which are also common pathological features of human idiopathic polymyositis (IPM). Defibrase-treated animals displayed extensive inflammation and fiber necrosis compared with the EAM group (histological score: 2.80 ±1.15 vs. 1.88 ±1.32, p < 0.05). Severity of inflammation of group B was mainly mild to moderate; 16.7% (2/12) of animals developed severe inflammation. Incidence of severe inflammation with a score up to 4 in group C was 40% (6/15).

Conclusions: Defibrase can exacerbate myosin-induced EAM; thus a new modified model was generated.

No MeSH data available.


Related in: MedlinePlus