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Syntenin is expressed in human gliomas and may correlate with tumor migration.

Zhong D, Ran J, Zhang X, Tan Y, Chen G, Tang W, Li X, Wang B - Arch Med Sci (2015)

Bottom Line: Syntenin has been demonstrated to be implicated in the migration, invasion and metastasis of many types of malignant tumors.The level of phosphorylated FAK at the tyrosine 397 site also elevated with the degree of tumor malignancy.Our results indicate that the enhanced expression of syntenin and phosphorylated FAK may correlate with the increase of the malignancy of human gliomas.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, the 1 Affiliated Hospital, Chongqing Medical University, Chongqing, China.

ABSTRACT

Introduction: Invasion is usually recognized as the main reason for the high recurrence and death rates of gliomas. Therefore, properly understanding the molecular mechanisms of migration and invasion of human gliomas has become a focus and will be helpful for the treatment of gliomas. Syntenin has been demonstrated to be implicated in the migration, invasion and metastasis of many types of malignant tumors. Therefore, we investigated the expression of syntenin in human gliomas and its relationship with glioma migration.

Material and methods: Immunohistochemistry, Western blot and real time-polymerase chain reaction (RT-PCR) were performed to detect the expression of syntenin in human gliomas. Phosphorylated FAK in human gliomas was examined by western blot.

Results: Scattered syntenin positive glioma cells were detected by immunohistochemistry in normal tissue. Syntenin expression in grade II, III and IV gliomas increased with the degree of tumor malignancy, and no syntenin expression was detected in grade I gliomas. The level of phosphorylated FAK at the tyrosine 397 site also elevated with the degree of tumor malignancy. There was a positive correlation between the syntenin level and the pathological grade of gliomas (r s = 0.896, p < 0.05). Phosphorylated FAK was also upregulated along with the stage of glioma progression and the increase of syntenin expression.

Conclusions: Our results indicate that the enhanced expression of syntenin and phosphorylated FAK may correlate with the increase of the malignancy of human gliomas. Syntenin may promote human glioma migration through interaction with FAK.

No MeSH data available.


Related in: MedlinePlus

Phosphorylation of FAK in different grades of glioma tissues. Protein was extracted from glioma tissues and western blot was performed using antibodies against p-FAK and β-actin
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Figure 0003: Phosphorylation of FAK in different grades of glioma tissues. Protein was extracted from glioma tissues and western blot was performed using antibodies against p-FAK and β-actin

Mentions: Activated FAK has been indicated as a positive modulator of cell motility and tumor migration and invasion, including gliomas [17, 20]. Therefore, we investigated the phosphorylation level of FAK in gliomas of different grades using western blot. In high-grade gliomas, the phosphorylation of FAK at Try397 was increased compared with that in low-grade gliomas (Figure 3).


Syntenin is expressed in human gliomas and may correlate with tumor migration.

Zhong D, Ran J, Zhang X, Tan Y, Chen G, Tang W, Li X, Wang B - Arch Med Sci (2015)

Phosphorylation of FAK in different grades of glioma tissues. Protein was extracted from glioma tissues and western blot was performed using antibodies against p-FAK and β-actin
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697043&req=5

Figure 0003: Phosphorylation of FAK in different grades of glioma tissues. Protein was extracted from glioma tissues and western blot was performed using antibodies against p-FAK and β-actin
Mentions: Activated FAK has been indicated as a positive modulator of cell motility and tumor migration and invasion, including gliomas [17, 20]. Therefore, we investigated the phosphorylation level of FAK in gliomas of different grades using western blot. In high-grade gliomas, the phosphorylation of FAK at Try397 was increased compared with that in low-grade gliomas (Figure 3).

Bottom Line: Syntenin has been demonstrated to be implicated in the migration, invasion and metastasis of many types of malignant tumors.The level of phosphorylated FAK at the tyrosine 397 site also elevated with the degree of tumor malignancy.Our results indicate that the enhanced expression of syntenin and phosphorylated FAK may correlate with the increase of the malignancy of human gliomas.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, the 1 Affiliated Hospital, Chongqing Medical University, Chongqing, China.

ABSTRACT

Introduction: Invasion is usually recognized as the main reason for the high recurrence and death rates of gliomas. Therefore, properly understanding the molecular mechanisms of migration and invasion of human gliomas has become a focus and will be helpful for the treatment of gliomas. Syntenin has been demonstrated to be implicated in the migration, invasion and metastasis of many types of malignant tumors. Therefore, we investigated the expression of syntenin in human gliomas and its relationship with glioma migration.

Material and methods: Immunohistochemistry, Western blot and real time-polymerase chain reaction (RT-PCR) were performed to detect the expression of syntenin in human gliomas. Phosphorylated FAK in human gliomas was examined by western blot.

Results: Scattered syntenin positive glioma cells were detected by immunohistochemistry in normal tissue. Syntenin expression in grade II, III and IV gliomas increased with the degree of tumor malignancy, and no syntenin expression was detected in grade I gliomas. The level of phosphorylated FAK at the tyrosine 397 site also elevated with the degree of tumor malignancy. There was a positive correlation between the syntenin level and the pathological grade of gliomas (r s = 0.896, p < 0.05). Phosphorylated FAK was also upregulated along with the stage of glioma progression and the increase of syntenin expression.

Conclusions: Our results indicate that the enhanced expression of syntenin and phosphorylated FAK may correlate with the increase of the malignancy of human gliomas. Syntenin may promote human glioma migration through interaction with FAK.

No MeSH data available.


Related in: MedlinePlus