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Induction of growth arrest in colorectal cancer cells by cold plasma and gold nanoparticles.

Irani S, Shahmirani Z, Atyabi SM, Mirpoor S - Arch Med Sci (2015)

Bottom Line: Plasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs).Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4',6-diamidino-2-phenylindole (DAPI) staining.It appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Introduction: Guided treatments with nanoparticles and cold atmospheric plasma are a new approach in cancer therapy. Plasma is an ionized gas that has reactive and energetic particles and can be produced in the laboratory by different methods.

Material and methods: Plasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs). Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4',6-diamidino-2-phenylindole (DAPI) staining.

Results: The results showed that cell death was increased significantly with p < 0.001 by cold atmospheric plasma in the presence of gold nanoparticles.

Conclusions: It appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy.

No MeSH data available.


Related in: MedlinePlus

The MTT test results after 48 h: cells were treated with cold plasma and gold nanoparticles. Gold nanoparticles concentration: 375 ppm. Cold plasma exposure time: 60, 90, 120, 180 s. ρ-value = 0.000
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Figure 0009: The MTT test results after 48 h: cells were treated with cold plasma and gold nanoparticles. Gold nanoparticles concentration: 375 ppm. Cold plasma exposure time: 60, 90, 120, 180 s. ρ-value = 0.000

Mentions: HCT-116 cells were treated with GNPs and cold atmospheric plasma and incubated for 24 h and 48 h. Then the supernatant was removed and MTT solution was added and incubated for 4 h. Dark-colored crystals were formed. By dissolving these crystals in DMSO solution, light absorption was measured with the ELISA reader (BioTech model). The results showed the cells treated by GNPs and then treated by cold atmospheric plasma. When the plasma treatment time increased, the number of viable cells significantly decreased (Figure 9).


Induction of growth arrest in colorectal cancer cells by cold plasma and gold nanoparticles.

Irani S, Shahmirani Z, Atyabi SM, Mirpoor S - Arch Med Sci (2015)

The MTT test results after 48 h: cells were treated with cold plasma and gold nanoparticles. Gold nanoparticles concentration: 375 ppm. Cold plasma exposure time: 60, 90, 120, 180 s. ρ-value = 0.000
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697042&req=5

Figure 0009: The MTT test results after 48 h: cells were treated with cold plasma and gold nanoparticles. Gold nanoparticles concentration: 375 ppm. Cold plasma exposure time: 60, 90, 120, 180 s. ρ-value = 0.000
Mentions: HCT-116 cells were treated with GNPs and cold atmospheric plasma and incubated for 24 h and 48 h. Then the supernatant was removed and MTT solution was added and incubated for 4 h. Dark-colored crystals were formed. By dissolving these crystals in DMSO solution, light absorption was measured with the ELISA reader (BioTech model). The results showed the cells treated by GNPs and then treated by cold atmospheric plasma. When the plasma treatment time increased, the number of viable cells significantly decreased (Figure 9).

Bottom Line: Plasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs).Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4',6-diamidino-2-phenylindole (DAPI) staining.It appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Introduction: Guided treatments with nanoparticles and cold atmospheric plasma are a new approach in cancer therapy. Plasma is an ionized gas that has reactive and energetic particles and can be produced in the laboratory by different methods.

Material and methods: Plasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs). Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4',6-diamidino-2-phenylindole (DAPI) staining.

Results: The results showed that cell death was increased significantly with p < 0.001 by cold atmospheric plasma in the presence of gold nanoparticles.

Conclusions: It appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy.

No MeSH data available.


Related in: MedlinePlus