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Induction of growth arrest in colorectal cancer cells by cold plasma and gold nanoparticles.

Irani S, Shahmirani Z, Atyabi SM, Mirpoor S - Arch Med Sci (2015)

Bottom Line: Plasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs).Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4',6-diamidino-2-phenylindole (DAPI) staining.It appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Introduction: Guided treatments with nanoparticles and cold atmospheric plasma are a new approach in cancer therapy. Plasma is an ionized gas that has reactive and energetic particles and can be produced in the laboratory by different methods.

Material and methods: Plasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs). Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4',6-diamidino-2-phenylindole (DAPI) staining.

Results: The results showed that cell death was increased significantly with p < 0.001 by cold atmospheric plasma in the presence of gold nanoparticles.

Conclusions: It appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy.

No MeSH data available.


Related in: MedlinePlus

Optical microscopy images 48 h after GNP treatment on HCT-116 cells (A). Exposure concentration: 375 ppm (B), 93.7 ppm (C), 46.8 ppm (D) and cells not treated with GNPs (E). Scale bar is 50 μm
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Figure 0005: Optical microscopy images 48 h after GNP treatment on HCT-116 cells (A). Exposure concentration: 375 ppm (B), 93.7 ppm (C), 46.8 ppm (D) and cells not treated with GNPs (E). Scale bar is 50 μm

Mentions: The results of optical microscopy showed the cells being treated with GNPs in different concentrations. When the concentration was increased, the cell viability decreased so that at 375 ppm concentration the smallest amount of living cells was observed, while at 93.7, 46.8 concentrations there were no significant reductions detected in the amount of living cells (Figure 5).


Induction of growth arrest in colorectal cancer cells by cold plasma and gold nanoparticles.

Irani S, Shahmirani Z, Atyabi SM, Mirpoor S - Arch Med Sci (2015)

Optical microscopy images 48 h after GNP treatment on HCT-116 cells (A). Exposure concentration: 375 ppm (B), 93.7 ppm (C), 46.8 ppm (D) and cells not treated with GNPs (E). Scale bar is 50 μm
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697042&req=5

Figure 0005: Optical microscopy images 48 h after GNP treatment on HCT-116 cells (A). Exposure concentration: 375 ppm (B), 93.7 ppm (C), 46.8 ppm (D) and cells not treated with GNPs (E). Scale bar is 50 μm
Mentions: The results of optical microscopy showed the cells being treated with GNPs in different concentrations. When the concentration was increased, the cell viability decreased so that at 375 ppm concentration the smallest amount of living cells was observed, while at 93.7, 46.8 concentrations there were no significant reductions detected in the amount of living cells (Figure 5).

Bottom Line: Plasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs).Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4',6-diamidino-2-phenylindole (DAPI) staining.It appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Introduction: Guided treatments with nanoparticles and cold atmospheric plasma are a new approach in cancer therapy. Plasma is an ionized gas that has reactive and energetic particles and can be produced in the laboratory by different methods.

Material and methods: Plasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs). Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4',6-diamidino-2-phenylindole (DAPI) staining.

Results: The results showed that cell death was increased significantly with p < 0.001 by cold atmospheric plasma in the presence of gold nanoparticles.

Conclusions: It appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy.

No MeSH data available.


Related in: MedlinePlus