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Induction of growth arrest in colorectal cancer cells by cold plasma and gold nanoparticles.

Irani S, Shahmirani Z, Atyabi SM, Mirpoor S - Arch Med Sci (2015)

Bottom Line: Plasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs).Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4',6-diamidino-2-phenylindole (DAPI) staining.It appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Introduction: Guided treatments with nanoparticles and cold atmospheric plasma are a new approach in cancer therapy. Plasma is an ionized gas that has reactive and energetic particles and can be produced in the laboratory by different methods.

Material and methods: Plasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs). Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4',6-diamidino-2-phenylindole (DAPI) staining.

Results: The results showed that cell death was increased significantly with p < 0.001 by cold atmospheric plasma in the presence of gold nanoparticles.

Conclusions: It appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy.

No MeSH data available.


Related in: MedlinePlus

Images obtained by optical microscopy 48 h after plasma treatment on HCT-116 cells. Exposure times: 40 s (A), 50 s (B), 60 s (C), 90 s (D), 120 s (E), 180 s (F) and cells not treated with cold plasma (G). Scale bar is 50 μm
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Figure 0002: Images obtained by optical microscopy 48 h after plasma treatment on HCT-116 cells. Exposure times: 40 s (A), 50 s (B), 60 s (C), 90 s (D), 120 s (E), 180 s (F) and cells not treated with cold plasma (G). Scale bar is 50 μm

Mentions: Plasma treatment of HCT-116 cells resulted in a significant reduction of cell proliferation after 24 h and 48 h treatments (Figure 2).


Induction of growth arrest in colorectal cancer cells by cold plasma and gold nanoparticles.

Irani S, Shahmirani Z, Atyabi SM, Mirpoor S - Arch Med Sci (2015)

Images obtained by optical microscopy 48 h after plasma treatment on HCT-116 cells. Exposure times: 40 s (A), 50 s (B), 60 s (C), 90 s (D), 120 s (E), 180 s (F) and cells not treated with cold plasma (G). Scale bar is 50 μm
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697042&req=5

Figure 0002: Images obtained by optical microscopy 48 h after plasma treatment on HCT-116 cells. Exposure times: 40 s (A), 50 s (B), 60 s (C), 90 s (D), 120 s (E), 180 s (F) and cells not treated with cold plasma (G). Scale bar is 50 μm
Mentions: Plasma treatment of HCT-116 cells resulted in a significant reduction of cell proliferation after 24 h and 48 h treatments (Figure 2).

Bottom Line: Plasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs).Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4',6-diamidino-2-phenylindole (DAPI) staining.It appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Introduction: Guided treatments with nanoparticles and cold atmospheric plasma are a new approach in cancer therapy. Plasma is an ionized gas that has reactive and energetic particles and can be produced in the laboratory by different methods.

Material and methods: Plasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs). Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4',6-diamidino-2-phenylindole (DAPI) staining.

Results: The results showed that cell death was increased significantly with p < 0.001 by cold atmospheric plasma in the presence of gold nanoparticles.

Conclusions: It appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy.

No MeSH data available.


Related in: MedlinePlus