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Effects of the Particulate Matter₂.₅ (PM₂.₅) on Lipoprotein Metabolism, Uptake and Degradation, and Embryo Toxicity.

Kim JY, Lee EY, Choi I, Kim J, Cho KH - Mol. Cells (2015)

Bottom Line: In the current study, we investigated the putative physiological effects of aqueous PM2.5 solution on lipoprotein metabolism.PM2.5 extract resulted in degradation and aggregation of high-density lipoprotein (HDL) as well as low-density lipoprotein (LDL); apoA-I in HDL aggregated and apo-B in LDL disappeared.In conclusion, water extract of PM2.5 induced oxidative stress as a precursor to cardiovascular toxicity, skin cell senescence, and embryonic toxicity via aggregation and proteolytic degradation of serum lipoproteins.

View Article: PubMed Central - PubMed

Affiliation: School of Biotechnology, Korea.

ABSTRACT
Particulate matter2.5 (PM2.5) is notorious for its strong toxic effects on the cardiovascular, skin, nervous, and reproduction systems. However, the molecular mechanism by which PM2.5 aggravates disease progression is poorly understood, especially in a water-soluble state. In the current study, we investigated the putative physiological effects of aqueous PM2.5 solution on lipoprotein metabolism. Collected PM2.5 from Seoul, Korea was dissolved in water, and the water extract (final 3 and 30 ppm) was treated to human serum lipoproteins, macrophages, and dermal cells. PM2.5 extract resulted in degradation and aggregation of high-density lipoprotein (HDL) as well as low-density lipoprotein (LDL); apoA-I in HDL aggregated and apo-B in LDL disappeared. PM2.5 treatment (final 30 ppm) also induced cellular uptake of oxidized LDL (oxLDL) into macrophages, especially in the presence of fructose (final 50 mM). Uptake of oxLDL along with production of reactive oxygen species was accelerated by PM2.5 solution in a dose-dependent manner. Further, PM2.5 solution caused cellular senescence in human dermal fibroblast cells. Microinjection of PM2.5 solution into zebrafish embryos induced severe mortality accompanied by impairment of skeletal development. In conclusion, water extract of PM2.5 induced oxidative stress as a precursor to cardiovascular toxicity, skin cell senescence, and embryonic toxicity via aggregation and proteolytic degradation of serum lipoproteins.

No MeSH data available.


Related in: MedlinePlus

Measurement of glycation extent of LDL modified by PM2.5 in the presence of fructose based on fluorescence intensity.
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f2-molce-38-12-1096: Measurement of glycation extent of LDL modified by PM2.5 in the presence of fructose based on fluorescence intensity.

Mentions: After 48 h of incubation, fluorescence intensity increased by 5.5-fold upon co-treatment with fructose and PM2.5 extract (Fig. 2) as well as by 2- and 4-fold upon PM2.5 and fructose co-treatment, respectively. These results suggest that extent of LDL glycation was accelerated by PM2.5 and fructose in a synergistic manner.


Effects of the Particulate Matter₂.₅ (PM₂.₅) on Lipoprotein Metabolism, Uptake and Degradation, and Embryo Toxicity.

Kim JY, Lee EY, Choi I, Kim J, Cho KH - Mol. Cells (2015)

Measurement of glycation extent of LDL modified by PM2.5 in the presence of fructose based on fluorescence intensity.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4697001&req=5

f2-molce-38-12-1096: Measurement of glycation extent of LDL modified by PM2.5 in the presence of fructose based on fluorescence intensity.
Mentions: After 48 h of incubation, fluorescence intensity increased by 5.5-fold upon co-treatment with fructose and PM2.5 extract (Fig. 2) as well as by 2- and 4-fold upon PM2.5 and fructose co-treatment, respectively. These results suggest that extent of LDL glycation was accelerated by PM2.5 and fructose in a synergistic manner.

Bottom Line: In the current study, we investigated the putative physiological effects of aqueous PM2.5 solution on lipoprotein metabolism.PM2.5 extract resulted in degradation and aggregation of high-density lipoprotein (HDL) as well as low-density lipoprotein (LDL); apoA-I in HDL aggregated and apo-B in LDL disappeared.In conclusion, water extract of PM2.5 induced oxidative stress as a precursor to cardiovascular toxicity, skin cell senescence, and embryonic toxicity via aggregation and proteolytic degradation of serum lipoproteins.

View Article: PubMed Central - PubMed

Affiliation: School of Biotechnology, Korea.

ABSTRACT
Particulate matter2.5 (PM2.5) is notorious for its strong toxic effects on the cardiovascular, skin, nervous, and reproduction systems. However, the molecular mechanism by which PM2.5 aggravates disease progression is poorly understood, especially in a water-soluble state. In the current study, we investigated the putative physiological effects of aqueous PM2.5 solution on lipoprotein metabolism. Collected PM2.5 from Seoul, Korea was dissolved in water, and the water extract (final 3 and 30 ppm) was treated to human serum lipoproteins, macrophages, and dermal cells. PM2.5 extract resulted in degradation and aggregation of high-density lipoprotein (HDL) as well as low-density lipoprotein (LDL); apoA-I in HDL aggregated and apo-B in LDL disappeared. PM2.5 treatment (final 30 ppm) also induced cellular uptake of oxidized LDL (oxLDL) into macrophages, especially in the presence of fructose (final 50 mM). Uptake of oxLDL along with production of reactive oxygen species was accelerated by PM2.5 solution in a dose-dependent manner. Further, PM2.5 solution caused cellular senescence in human dermal fibroblast cells. Microinjection of PM2.5 solution into zebrafish embryos induced severe mortality accompanied by impairment of skeletal development. In conclusion, water extract of PM2.5 induced oxidative stress as a precursor to cardiovascular toxicity, skin cell senescence, and embryonic toxicity via aggregation and proteolytic degradation of serum lipoproteins.

No MeSH data available.


Related in: MedlinePlus