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Genetic Map of Triticale Integrating Microsatellite, DArT and SNP Markers.

Tyrka M, Tyrka D, Wędzony M - PLoS ONE (2015)

Bottom Line: No markers specific to chromosome 7R were found, instead mosaic linkage group composed of 1880 highly distorted markers (116 bins) from 10 wheat chromosomes was identified.A total of 512 (31.7%) bin markers showed significant (P < 0.05) segregation distortion across all chromosomes.The high-density genetic map of triticale will facilitate fine mapping of quantitative trait loci, the identification of candidate genes and map-based cloning.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Biotechnology, Faculty of Chemistry, Rzeszow University of Technology, Rzeszow, Poland.

ABSTRACT
Triticale (×Triticosecale Wittm) is an economically important crop for fodder and biomass production. To facilitate the identification of markers for agronomically important traits and for genetic and genomic characteristics of this species, a new high-density genetic linkage map of triticale was constructed using doubled haploid (DH) population derived from a cross between cultivars 'Hewo' and 'Magnat'. The map consists of 1615 bin markers, that represent 50 simple sequence repeat (SSR), 842 diversity array technology (DArT), and 16888 DArTseq markers mapped onto 20 linkage groups assigned to the A, B, and R genomes of triticale. No markers specific to chromosome 7R were found, instead mosaic linkage group composed of 1880 highly distorted markers (116 bins) from 10 wheat chromosomes was identified. The genetic map covers 4907 cM with a mean distance between two bins of 3.0 cM. Comparative analysis in respect to published maps of wheat, rye and triticale revealed possible deletions in chromosomes 4B, 5A, and 6A, as well as inversion in chromosome 7B. The number of bin markers in each chromosome varied from 24 in chromosome 3R to 147 in chromosome 6R. The length of individual chromosomes ranged between 50.7 cM for chromosome 2R and 386.2 cM for chromosome 7B. A total of 512 (31.7%) bin markers showed significant (P < 0.05) segregation distortion across all chromosomes. The number of 8 the segregation distorted regions (SDRs) were identified on 1A, 7A, 1B, 2B, 7B (2 SDRs), 5R and 6R chromosomes. The high-density genetic map of triticale will facilitate fine mapping of quantitative trait loci, the identification of candidate genes and map-based cloning.

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Related in: MedlinePlus

Genetic linkage map of the triticale R genome obtained with 89 doubled haploid lines of the ‘Hewo’ × ‘Magnat’ population comprising SSR, DArT, and DArTseq markers.
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pone.0145714.g005: Genetic linkage map of the triticale R genome obtained with 89 doubled haploid lines of the ‘Hewo’ × ‘Magnat’ population comprising SSR, DArT, and DArTseq markers.

Mentions: R genome of HM-DH population consisted of 6 linkage groups with 7R chromosome missing (Fig 5). Chromosome 1R consisted of two clusters of markers corresponding to short and long arm put into single chromosome at distance of 47.1 cM. DArTseq markers for R genome are not annotated with chromosome position. Then, localization of DArT markers on consensus map of triticale [46] and rye [16] was used to identify linkage groups (Fig 6). Chromosomes 1R, 4R, 5R and 6R showed good agreement with rye and triticale maps expressed by high correlation coefficients (0.89–0.98). Fragment of chromosome 1R of HM-DH equal to 7cM corresponds to 53 cM region in the genetic map of rye (from 213 to 266 cM). Colinearity of markers from chromosomes 2R and 3R was low (0.78, and 0.52, respectively). These linkage groups were the shortest in R genome of HM-DH population (50.7 and 60.9, respectively) with markers of suppressed recombination in respect to rye genetic map. Suppressed recombination in triticale vs rye is also well visible in case of 4R and 5R where in rye markers spanning 300 cM are packed in 50 cM fragments of HM-DH population.


Genetic Map of Triticale Integrating Microsatellite, DArT and SNP Markers.

Tyrka M, Tyrka D, Wędzony M - PLoS ONE (2015)

Genetic linkage map of the triticale R genome obtained with 89 doubled haploid lines of the ‘Hewo’ × ‘Magnat’ population comprising SSR, DArT, and DArTseq markers.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696847&req=5

pone.0145714.g005: Genetic linkage map of the triticale R genome obtained with 89 doubled haploid lines of the ‘Hewo’ × ‘Magnat’ population comprising SSR, DArT, and DArTseq markers.
Mentions: R genome of HM-DH population consisted of 6 linkage groups with 7R chromosome missing (Fig 5). Chromosome 1R consisted of two clusters of markers corresponding to short and long arm put into single chromosome at distance of 47.1 cM. DArTseq markers for R genome are not annotated with chromosome position. Then, localization of DArT markers on consensus map of triticale [46] and rye [16] was used to identify linkage groups (Fig 6). Chromosomes 1R, 4R, 5R and 6R showed good agreement with rye and triticale maps expressed by high correlation coefficients (0.89–0.98). Fragment of chromosome 1R of HM-DH equal to 7cM corresponds to 53 cM region in the genetic map of rye (from 213 to 266 cM). Colinearity of markers from chromosomes 2R and 3R was low (0.78, and 0.52, respectively). These linkage groups were the shortest in R genome of HM-DH population (50.7 and 60.9, respectively) with markers of suppressed recombination in respect to rye genetic map. Suppressed recombination in triticale vs rye is also well visible in case of 4R and 5R where in rye markers spanning 300 cM are packed in 50 cM fragments of HM-DH population.

Bottom Line: No markers specific to chromosome 7R were found, instead mosaic linkage group composed of 1880 highly distorted markers (116 bins) from 10 wheat chromosomes was identified.A total of 512 (31.7%) bin markers showed significant (P < 0.05) segregation distortion across all chromosomes.The high-density genetic map of triticale will facilitate fine mapping of quantitative trait loci, the identification of candidate genes and map-based cloning.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Biotechnology, Faculty of Chemistry, Rzeszow University of Technology, Rzeszow, Poland.

ABSTRACT
Triticale (×Triticosecale Wittm) is an economically important crop for fodder and biomass production. To facilitate the identification of markers for agronomically important traits and for genetic and genomic characteristics of this species, a new high-density genetic linkage map of triticale was constructed using doubled haploid (DH) population derived from a cross between cultivars 'Hewo' and 'Magnat'. The map consists of 1615 bin markers, that represent 50 simple sequence repeat (SSR), 842 diversity array technology (DArT), and 16888 DArTseq markers mapped onto 20 linkage groups assigned to the A, B, and R genomes of triticale. No markers specific to chromosome 7R were found, instead mosaic linkage group composed of 1880 highly distorted markers (116 bins) from 10 wheat chromosomes was identified. The genetic map covers 4907 cM with a mean distance between two bins of 3.0 cM. Comparative analysis in respect to published maps of wheat, rye and triticale revealed possible deletions in chromosomes 4B, 5A, and 6A, as well as inversion in chromosome 7B. The number of bin markers in each chromosome varied from 24 in chromosome 3R to 147 in chromosome 6R. The length of individual chromosomes ranged between 50.7 cM for chromosome 2R and 386.2 cM for chromosome 7B. A total of 512 (31.7%) bin markers showed significant (P < 0.05) segregation distortion across all chromosomes. The number of 8 the segregation distorted regions (SDRs) were identified on 1A, 7A, 1B, 2B, 7B (2 SDRs), 5R and 6R chromosomes. The high-density genetic map of triticale will facilitate fine mapping of quantitative trait loci, the identification of candidate genes and map-based cloning.

Show MeSH
Related in: MedlinePlus