Limits...
Genetic Map of Triticale Integrating Microsatellite, DArT and SNP Markers.

Tyrka M, Tyrka D, Wędzony M - PLoS ONE (2015)

Bottom Line: No markers specific to chromosome 7R were found, instead mosaic linkage group composed of 1880 highly distorted markers (116 bins) from 10 wheat chromosomes was identified.A total of 512 (31.7%) bin markers showed significant (P < 0.05) segregation distortion across all chromosomes.The high-density genetic map of triticale will facilitate fine mapping of quantitative trait loci, the identification of candidate genes and map-based cloning.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Biotechnology, Faculty of Chemistry, Rzeszow University of Technology, Rzeszow, Poland.

ABSTRACT
Triticale (×Triticosecale Wittm) is an economically important crop for fodder and biomass production. To facilitate the identification of markers for agronomically important traits and for genetic and genomic characteristics of this species, a new high-density genetic linkage map of triticale was constructed using doubled haploid (DH) population derived from a cross between cultivars 'Hewo' and 'Magnat'. The map consists of 1615 bin markers, that represent 50 simple sequence repeat (SSR), 842 diversity array technology (DArT), and 16888 DArTseq markers mapped onto 20 linkage groups assigned to the A, B, and R genomes of triticale. No markers specific to chromosome 7R were found, instead mosaic linkage group composed of 1880 highly distorted markers (116 bins) from 10 wheat chromosomes was identified. The genetic map covers 4907 cM with a mean distance between two bins of 3.0 cM. Comparative analysis in respect to published maps of wheat, rye and triticale revealed possible deletions in chromosomes 4B, 5A, and 6A, as well as inversion in chromosome 7B. The number of bin markers in each chromosome varied from 24 in chromosome 3R to 147 in chromosome 6R. The length of individual chromosomes ranged between 50.7 cM for chromosome 2R and 386.2 cM for chromosome 7B. A total of 512 (31.7%) bin markers showed significant (P < 0.05) segregation distortion across all chromosomes. The number of 8 the segregation distorted regions (SDRs) were identified on 1A, 7A, 1B, 2B, 7B (2 SDRs), 5R and 6R chromosomes. The high-density genetic map of triticale will facilitate fine mapping of quantitative trait loci, the identification of candidate genes and map-based cloning.

Show MeSH

Related in: MedlinePlus

Genetic linkage map of the triticale B genome obtained with 89 doubled haploid lines of the ‘Hewo’ × ‘Magnat’ population comprising SSR, DArT, and DArTseq markers.The ruler on the left indicates distances in centiMorgans (Kosambi).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4696847&req=5

pone.0145714.g002: Genetic linkage map of the triticale B genome obtained with 89 doubled haploid lines of the ‘Hewo’ × ‘Magnat’ population comprising SSR, DArT, and DArTseq markers.The ruler on the left indicates distances in centiMorgans (Kosambi).

Mentions: The genetic map of DH-HM showed good coverage of the A and B genome chromosomes, apart from 6A. Compared with positions on consensus map of wheat, most shared markers showed highly consistent orders with correlation coefficient exceeding 0.900. The maps in Figs 1 and 2 include representations of all markers assigned to the identified linkage groups, grouped by genome. To simplify the map, redundant co-located markers were represented by a single, representative bin marker with the total number of markers represented (S1 Table) given in parentheses after the marker name. Genetic map was constructed for bin markers and redundant markers were added to saturate the map and to supplement sequence information (S2 and S3 Tables). Loci of DArTseq markers on chromosomes from the A and B genomes were compared with reference positions for wheat provided within service (Figs 3 and 4).


Genetic Map of Triticale Integrating Microsatellite, DArT and SNP Markers.

Tyrka M, Tyrka D, Wędzony M - PLoS ONE (2015)

Genetic linkage map of the triticale B genome obtained with 89 doubled haploid lines of the ‘Hewo’ × ‘Magnat’ population comprising SSR, DArT, and DArTseq markers.The ruler on the left indicates distances in centiMorgans (Kosambi).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696847&req=5

pone.0145714.g002: Genetic linkage map of the triticale B genome obtained with 89 doubled haploid lines of the ‘Hewo’ × ‘Magnat’ population comprising SSR, DArT, and DArTseq markers.The ruler on the left indicates distances in centiMorgans (Kosambi).
Mentions: The genetic map of DH-HM showed good coverage of the A and B genome chromosomes, apart from 6A. Compared with positions on consensus map of wheat, most shared markers showed highly consistent orders with correlation coefficient exceeding 0.900. The maps in Figs 1 and 2 include representations of all markers assigned to the identified linkage groups, grouped by genome. To simplify the map, redundant co-located markers were represented by a single, representative bin marker with the total number of markers represented (S1 Table) given in parentheses after the marker name. Genetic map was constructed for bin markers and redundant markers were added to saturate the map and to supplement sequence information (S2 and S3 Tables). Loci of DArTseq markers on chromosomes from the A and B genomes were compared with reference positions for wheat provided within service (Figs 3 and 4).

Bottom Line: No markers specific to chromosome 7R were found, instead mosaic linkage group composed of 1880 highly distorted markers (116 bins) from 10 wheat chromosomes was identified.A total of 512 (31.7%) bin markers showed significant (P < 0.05) segregation distortion across all chromosomes.The high-density genetic map of triticale will facilitate fine mapping of quantitative trait loci, the identification of candidate genes and map-based cloning.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Biotechnology, Faculty of Chemistry, Rzeszow University of Technology, Rzeszow, Poland.

ABSTRACT
Triticale (×Triticosecale Wittm) is an economically important crop for fodder and biomass production. To facilitate the identification of markers for agronomically important traits and for genetic and genomic characteristics of this species, a new high-density genetic linkage map of triticale was constructed using doubled haploid (DH) population derived from a cross between cultivars 'Hewo' and 'Magnat'. The map consists of 1615 bin markers, that represent 50 simple sequence repeat (SSR), 842 diversity array technology (DArT), and 16888 DArTseq markers mapped onto 20 linkage groups assigned to the A, B, and R genomes of triticale. No markers specific to chromosome 7R were found, instead mosaic linkage group composed of 1880 highly distorted markers (116 bins) from 10 wheat chromosomes was identified. The genetic map covers 4907 cM with a mean distance between two bins of 3.0 cM. Comparative analysis in respect to published maps of wheat, rye and triticale revealed possible deletions in chromosomes 4B, 5A, and 6A, as well as inversion in chromosome 7B. The number of bin markers in each chromosome varied from 24 in chromosome 3R to 147 in chromosome 6R. The length of individual chromosomes ranged between 50.7 cM for chromosome 2R and 386.2 cM for chromosome 7B. A total of 512 (31.7%) bin markers showed significant (P < 0.05) segregation distortion across all chromosomes. The number of 8 the segregation distorted regions (SDRs) were identified on 1A, 7A, 1B, 2B, 7B (2 SDRs), 5R and 6R chromosomes. The high-density genetic map of triticale will facilitate fine mapping of quantitative trait loci, the identification of candidate genes and map-based cloning.

Show MeSH
Related in: MedlinePlus