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Neurotransmitter Specific, Cellular-Resolution Functional Brain Mapping Using Receptor Coated Nanoparticles: Assessment of the Possibility.

Forati E, Sabouni A, Ray S, Head B, Schoen C, Sievenpiper D - PLoS ONE (2015)

Bottom Line: Gold nanoparticles (GNPs) with two different geometries (sphere and rod) and quantum dots (QDs) with different sizes were studied along with three different neurotransmitters: dopamine, gamma-Aminobutyric acid (GABA), and glycine.The absorption/emission spectra of GNPs and QDs before and after binding of neurotransmitters and their corresponding receptors are reported.The results using QDs and nanorods with diameter 25nm and aspect rations larger than three were promising for the development of the proposed functional brain mapping approach.

View Article: PubMed Central - PubMed

Affiliation: Electrical and Computer Engineering Department, University of California San Diego, La Jolla, CA 92098, United States of America.

ABSTRACT
Receptor coated resonant nanoparticles and quantum dots are proposed to provide a cellular-level resolution image of neural activities inside the brain. The functionalized nanoparticles and quantum dots in this approach will selectively bind to different neurotransmitters in the extra-synaptic regions of neurons. This allows us to detect neural activities in real time by monitoring the nanoparticles and quantum dots optically. Gold nanoparticles (GNPs) with two different geometries (sphere and rod) and quantum dots (QDs) with different sizes were studied along with three different neurotransmitters: dopamine, gamma-Aminobutyric acid (GABA), and glycine. The absorption/emission spectra of GNPs and QDs before and after binding of neurotransmitters and their corresponding receptors are reported. The results using QDs and nanorods with diameter 25nm and aspect rations larger than three were promising for the development of the proposed functional brain mapping approach.

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a) Absorption spectra of NR5 and NR6 before and after functionalizing with the receptors, and b) using the mixture of the functionalized GNPs to detect both Dop and Gab.The numbers in parenthesizes report the peak wavelengths of each curve in units of nm.
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pone.0145852.g006: a) Absorption spectra of NR5 and NR6 before and after functionalizing with the receptors, and b) using the mixture of the functionalized GNPs to detect both Dop and Gab.The numbers in parenthesizes report the peak wavelengths of each curve in units of nm.

Mentions: As a further investigation, NR5 and NR6 were functionalized with Antidop and Antigly, respectively. The two functionalized GNPs were then mixed together and Gly or Dop was added to the mixture consequently. The idea in this measurement set was to examine the possibility of discerning neurotransmitters using a mixture GNPs with different sizes so that each size was functionalized with a specific receptor. Fig 6(a) shows the spectra of NR5 and NR6 before and after binding to the receptors. The shift in the peak wavelength confirmed proper GNP functionalizations. Note that unlike the nanorods with diameter 25nm (and nanospheres), the longitudinal resonance of the nanorods with diameter 10nm experienced a blue-shift post binding of Antidop (this was also observed in measurements using different nanorods). On the other hand, after mixing the two functionalized GNPs, both of the longitudinal peaks experienced a larger redshift (canceling the blue shift of the second peak), which could be due to the charge effects in the solution. Fig 6(b) includes the spectrum of the mixture of the bare nanorods with two longitudinal resonance peaks at 667nm and 957nm which the first peak moved to 683nm post binding of antibodies. The first and the second peaks redshifted 7nm and 33nm, respectively, post binding of Gly which were different form the 13nm and the 52nm redshifts post binding of Dop.


Neurotransmitter Specific, Cellular-Resolution Functional Brain Mapping Using Receptor Coated Nanoparticles: Assessment of the Possibility.

Forati E, Sabouni A, Ray S, Head B, Schoen C, Sievenpiper D - PLoS ONE (2015)

a) Absorption spectra of NR5 and NR6 before and after functionalizing with the receptors, and b) using the mixture of the functionalized GNPs to detect both Dop and Gab.The numbers in parenthesizes report the peak wavelengths of each curve in units of nm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696845&req=5

pone.0145852.g006: a) Absorption spectra of NR5 and NR6 before and after functionalizing with the receptors, and b) using the mixture of the functionalized GNPs to detect both Dop and Gab.The numbers in parenthesizes report the peak wavelengths of each curve in units of nm.
Mentions: As a further investigation, NR5 and NR6 were functionalized with Antidop and Antigly, respectively. The two functionalized GNPs were then mixed together and Gly or Dop was added to the mixture consequently. The idea in this measurement set was to examine the possibility of discerning neurotransmitters using a mixture GNPs with different sizes so that each size was functionalized with a specific receptor. Fig 6(a) shows the spectra of NR5 and NR6 before and after binding to the receptors. The shift in the peak wavelength confirmed proper GNP functionalizations. Note that unlike the nanorods with diameter 25nm (and nanospheres), the longitudinal resonance of the nanorods with diameter 10nm experienced a blue-shift post binding of Antidop (this was also observed in measurements using different nanorods). On the other hand, after mixing the two functionalized GNPs, both of the longitudinal peaks experienced a larger redshift (canceling the blue shift of the second peak), which could be due to the charge effects in the solution. Fig 6(b) includes the spectrum of the mixture of the bare nanorods with two longitudinal resonance peaks at 667nm and 957nm which the first peak moved to 683nm post binding of antibodies. The first and the second peaks redshifted 7nm and 33nm, respectively, post binding of Gly which were different form the 13nm and the 52nm redshifts post binding of Dop.

Bottom Line: Gold nanoparticles (GNPs) with two different geometries (sphere and rod) and quantum dots (QDs) with different sizes were studied along with three different neurotransmitters: dopamine, gamma-Aminobutyric acid (GABA), and glycine.The absorption/emission spectra of GNPs and QDs before and after binding of neurotransmitters and their corresponding receptors are reported.The results using QDs and nanorods with diameter 25nm and aspect rations larger than three were promising for the development of the proposed functional brain mapping approach.

View Article: PubMed Central - PubMed

Affiliation: Electrical and Computer Engineering Department, University of California San Diego, La Jolla, CA 92098, United States of America.

ABSTRACT
Receptor coated resonant nanoparticles and quantum dots are proposed to provide a cellular-level resolution image of neural activities inside the brain. The functionalized nanoparticles and quantum dots in this approach will selectively bind to different neurotransmitters in the extra-synaptic regions of neurons. This allows us to detect neural activities in real time by monitoring the nanoparticles and quantum dots optically. Gold nanoparticles (GNPs) with two different geometries (sphere and rod) and quantum dots (QDs) with different sizes were studied along with three different neurotransmitters: dopamine, gamma-Aminobutyric acid (GABA), and glycine. The absorption/emission spectra of GNPs and QDs before and after binding of neurotransmitters and their corresponding receptors are reported. The results using QDs and nanorods with diameter 25nm and aspect rations larger than three were promising for the development of the proposed functional brain mapping approach.

Show MeSH
Related in: MedlinePlus