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Neurotransmitter Specific, Cellular-Resolution Functional Brain Mapping Using Receptor Coated Nanoparticles: Assessment of the Possibility.

Forati E, Sabouni A, Ray S, Head B, Schoen C, Sievenpiper D - PLoS ONE (2015)

Bottom Line: Gold nanoparticles (GNPs) with two different geometries (sphere and rod) and quantum dots (QDs) with different sizes were studied along with three different neurotransmitters: dopamine, gamma-Aminobutyric acid (GABA), and glycine.The absorption/emission spectra of GNPs and QDs before and after binding of neurotransmitters and their corresponding receptors are reported.The results using QDs and nanorods with diameter 25nm and aspect rations larger than three were promising for the development of the proposed functional brain mapping approach.

View Article: PubMed Central - PubMed

Affiliation: Electrical and Computer Engineering Department, University of California San Diego, La Jolla, CA 92098, United States of America.

ABSTRACT
Receptor coated resonant nanoparticles and quantum dots are proposed to provide a cellular-level resolution image of neural activities inside the brain. The functionalized nanoparticles and quantum dots in this approach will selectively bind to different neurotransmitters in the extra-synaptic regions of neurons. This allows us to detect neural activities in real time by monitoring the nanoparticles and quantum dots optically. Gold nanoparticles (GNPs) with two different geometries (sphere and rod) and quantum dots (QDs) with different sizes were studied along with three different neurotransmitters: dopamine, gamma-Aminobutyric acid (GABA), and glycine. The absorption/emission spectra of GNPs and QDs before and after binding of neurotransmitters and their corresponding receptors are reported. The results using QDs and nanorods with diameter 25nm and aspect rations larger than three were promising for the development of the proposed functional brain mapping approach.

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Related in: MedlinePlus

Example TEM images of the studies GNPs: NR6 (left), and NS5 (right) as introduced in Table 1.
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pone.0145852.g003: Example TEM images of the studies GNPs: NR6 (left), and NS5 (right) as introduced in Table 1.

Mentions: Several gold nanoparticles were functionalized using different neurotransmitter receptors, and their absorption spectra are reported pre and post binding of neurotransmitters. We used commercially available GNPs (both spheres and rods) in order to keep the experiments reproducible for future investigations without concerns about the variabilities in nanoparticles synthesis. Table 1 lists the nanoparticles used along with their basic characteristics and Table 2 contains the different neurotransmitters and receptors which were used in this study. Sample TEM images of the studied GNPs are shown in Fig 3 as well.


Neurotransmitter Specific, Cellular-Resolution Functional Brain Mapping Using Receptor Coated Nanoparticles: Assessment of the Possibility.

Forati E, Sabouni A, Ray S, Head B, Schoen C, Sievenpiper D - PLoS ONE (2015)

Example TEM images of the studies GNPs: NR6 (left), and NS5 (right) as introduced in Table 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696845&req=5

pone.0145852.g003: Example TEM images of the studies GNPs: NR6 (left), and NS5 (right) as introduced in Table 1.
Mentions: Several gold nanoparticles were functionalized using different neurotransmitter receptors, and their absorption spectra are reported pre and post binding of neurotransmitters. We used commercially available GNPs (both spheres and rods) in order to keep the experiments reproducible for future investigations without concerns about the variabilities in nanoparticles synthesis. Table 1 lists the nanoparticles used along with their basic characteristics and Table 2 contains the different neurotransmitters and receptors which were used in this study. Sample TEM images of the studied GNPs are shown in Fig 3 as well.

Bottom Line: Gold nanoparticles (GNPs) with two different geometries (sphere and rod) and quantum dots (QDs) with different sizes were studied along with three different neurotransmitters: dopamine, gamma-Aminobutyric acid (GABA), and glycine.The absorption/emission spectra of GNPs and QDs before and after binding of neurotransmitters and their corresponding receptors are reported.The results using QDs and nanorods with diameter 25nm and aspect rations larger than three were promising for the development of the proposed functional brain mapping approach.

View Article: PubMed Central - PubMed

Affiliation: Electrical and Computer Engineering Department, University of California San Diego, La Jolla, CA 92098, United States of America.

ABSTRACT
Receptor coated resonant nanoparticles and quantum dots are proposed to provide a cellular-level resolution image of neural activities inside the brain. The functionalized nanoparticles and quantum dots in this approach will selectively bind to different neurotransmitters in the extra-synaptic regions of neurons. This allows us to detect neural activities in real time by monitoring the nanoparticles and quantum dots optically. Gold nanoparticles (GNPs) with two different geometries (sphere and rod) and quantum dots (QDs) with different sizes were studied along with three different neurotransmitters: dopamine, gamma-Aminobutyric acid (GABA), and glycine. The absorption/emission spectra of GNPs and QDs before and after binding of neurotransmitters and their corresponding receptors are reported. The results using QDs and nanorods with diameter 25nm and aspect rations larger than three were promising for the development of the proposed functional brain mapping approach.

Show MeSH
Related in: MedlinePlus