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Neurotransmitter Specific, Cellular-Resolution Functional Brain Mapping Using Receptor Coated Nanoparticles: Assessment of the Possibility.

Forati E, Sabouni A, Ray S, Head B, Schoen C, Sievenpiper D - PLoS ONE (2015)

Bottom Line: Gold nanoparticles (GNPs) with two different geometries (sphere and rod) and quantum dots (QDs) with different sizes were studied along with three different neurotransmitters: dopamine, gamma-Aminobutyric acid (GABA), and glycine.The absorption/emission spectra of GNPs and QDs before and after binding of neurotransmitters and their corresponding receptors are reported.The results using QDs and nanorods with diameter 25nm and aspect rations larger than three were promising for the development of the proposed functional brain mapping approach.

View Article: PubMed Central - PubMed

Affiliation: Electrical and Computer Engineering Department, University of California San Diego, La Jolla, CA 92098, United States of America.

ABSTRACT
Receptor coated resonant nanoparticles and quantum dots are proposed to provide a cellular-level resolution image of neural activities inside the brain. The functionalized nanoparticles and quantum dots in this approach will selectively bind to different neurotransmitters in the extra-synaptic regions of neurons. This allows us to detect neural activities in real time by monitoring the nanoparticles and quantum dots optically. Gold nanoparticles (GNPs) with two different geometries (sphere and rod) and quantum dots (QDs) with different sizes were studied along with three different neurotransmitters: dopamine, gamma-Aminobutyric acid (GABA), and glycine. The absorption/emission spectra of GNPs and QDs before and after binding of neurotransmitters and their corresponding receptors are reported. The results using QDs and nanorods with diameter 25nm and aspect rations larger than three were promising for the development of the proposed functional brain mapping approach.

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Related in: MedlinePlus

The proposed concept of optical real time imaging of neural activities using functionalized resonant nano-structures.Figure is drawn by A.S.
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pone.0145852.g002: The proposed concept of optical real time imaging of neural activities using functionalized resonant nano-structures.Figure is drawn by A.S.

Mentions: The idea in this work is to develop a technique whereby extra-synaptic dopamine (and other types of neurotransmitters) can be detected with cellular level resolution based on the optical properties of plasmonic nanoparticels, i.e. Localized Surface Plasmon Resonance (LSPR), or QDs. One advantage of this approach is that multiple neurotransmitters can potentially be identified simultaneously by using different nanoparticles. Resonant nanoparticles and QDs in this approach are functionalized with the appropriate receptors, as shown in Fig 1. This paper is specifically focused on developing solution-based dopamine sensors. As Fig 2 depicts the proposed idea schematically, functionalized nanoparticles are to be distributed over the neurons and within the synapses. During neural activities, the sparsely released dopamine in the extra-synaptic region of neurons will bind to the functionalized nanoparticles and will be detected optically by means of spectroscopy (reflection or photoluminescence). It is expected that the binding of neurotransmitters to the nanoparticles would change the optically reflected/transmitted light that can be detected effectively. In the following, we first study GNPs as the neurotransmitter’s detectors. Then, in the last section, we consider QDs and compare them with GNPs.


Neurotransmitter Specific, Cellular-Resolution Functional Brain Mapping Using Receptor Coated Nanoparticles: Assessment of the Possibility.

Forati E, Sabouni A, Ray S, Head B, Schoen C, Sievenpiper D - PLoS ONE (2015)

The proposed concept of optical real time imaging of neural activities using functionalized resonant nano-structures.Figure is drawn by A.S.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696845&req=5

pone.0145852.g002: The proposed concept of optical real time imaging of neural activities using functionalized resonant nano-structures.Figure is drawn by A.S.
Mentions: The idea in this work is to develop a technique whereby extra-synaptic dopamine (and other types of neurotransmitters) can be detected with cellular level resolution based on the optical properties of plasmonic nanoparticels, i.e. Localized Surface Plasmon Resonance (LSPR), or QDs. One advantage of this approach is that multiple neurotransmitters can potentially be identified simultaneously by using different nanoparticles. Resonant nanoparticles and QDs in this approach are functionalized with the appropriate receptors, as shown in Fig 1. This paper is specifically focused on developing solution-based dopamine sensors. As Fig 2 depicts the proposed idea schematically, functionalized nanoparticles are to be distributed over the neurons and within the synapses. During neural activities, the sparsely released dopamine in the extra-synaptic region of neurons will bind to the functionalized nanoparticles and will be detected optically by means of spectroscopy (reflection or photoluminescence). It is expected that the binding of neurotransmitters to the nanoparticles would change the optically reflected/transmitted light that can be detected effectively. In the following, we first study GNPs as the neurotransmitter’s detectors. Then, in the last section, we consider QDs and compare them with GNPs.

Bottom Line: Gold nanoparticles (GNPs) with two different geometries (sphere and rod) and quantum dots (QDs) with different sizes were studied along with three different neurotransmitters: dopamine, gamma-Aminobutyric acid (GABA), and glycine.The absorption/emission spectra of GNPs and QDs before and after binding of neurotransmitters and their corresponding receptors are reported.The results using QDs and nanorods with diameter 25nm and aspect rations larger than three were promising for the development of the proposed functional brain mapping approach.

View Article: PubMed Central - PubMed

Affiliation: Electrical and Computer Engineering Department, University of California San Diego, La Jolla, CA 92098, United States of America.

ABSTRACT
Receptor coated resonant nanoparticles and quantum dots are proposed to provide a cellular-level resolution image of neural activities inside the brain. The functionalized nanoparticles and quantum dots in this approach will selectively bind to different neurotransmitters in the extra-synaptic regions of neurons. This allows us to detect neural activities in real time by monitoring the nanoparticles and quantum dots optically. Gold nanoparticles (GNPs) with two different geometries (sphere and rod) and quantum dots (QDs) with different sizes were studied along with three different neurotransmitters: dopamine, gamma-Aminobutyric acid (GABA), and glycine. The absorption/emission spectra of GNPs and QDs before and after binding of neurotransmitters and their corresponding receptors are reported. The results using QDs and nanorods with diameter 25nm and aspect rations larger than three were promising for the development of the proposed functional brain mapping approach.

Show MeSH
Related in: MedlinePlus