Limits...
The Effect of Oxytocin on Social and Non-Social Behaviour and Striatal Protein Expression in C57BL/6N Mice.

Zhang X, Li Q, Zhang M, Lam S, Sham PC, Bu B, Chua SE, Wang W, McAlonan GM - PLoS ONE (2015)

Bottom Line: However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes.With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics.Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), 1095 Jiefang Ave., Wuhan, 430030, P.R. China.

ABSTRACT
Oxytocin has been suggested as a promising new treatment for neurodevelopmental disorders. However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes. Here we investigated the effects of a range of oxytocin doses on social and non-social behaviours in C57BL/6N mice of both sexes. As the striatum modulates social and non-social behaviours, and is implicated in neurodevelopmental disorders, we also conducted a pilot exploration of changes in striatal protein expression elicited by oxytocin. Oxytocin increased prepulse inhibition of startle but attenuated the recognition memory in male C57BL/6N mice. It increased social interaction time and suppressed the amphetamine locomotor response in both sexes. The striatum proteome following oxytocin exposure could be clearly discriminated from saline controls. With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics. The proteins affected by oxytocin could be broadly categorized as those that modulate glutamatergic, GABAergic or dopaminergic signalling and those that mediate cytoskeleton dynamics. Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

Show MeSH

Related in: MedlinePlus

Striatum proteomics and western blot studies.(A) Multivariate analysis: partial least squares—discriminative analysis (PLS-DA) carried out using SIMCA-P+12.0 software (Umetrics). The striatal protein profile following oxytocin exposure could be clearly differentiated from saline (controls). The XY axis shows the coordinates of the protein distribution in the partial least squares—discriminative analysis (B) Western blot gel images of target proteins. (C) The relative expression of the target proteins/β-actin, Columns show mean±SEM (n = 9 for saline exposure; n = 8 for oxytocin exposure). * p<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4696826&req=5

pone.0145638.g007: Striatum proteomics and western blot studies.(A) Multivariate analysis: partial least squares—discriminative analysis (PLS-DA) carried out using SIMCA-P+12.0 software (Umetrics). The striatal protein profile following oxytocin exposure could be clearly differentiated from saline (controls). The XY axis shows the coordinates of the protein distribution in the partial least squares—discriminative analysis (B) Western blot gel images of target proteins. (C) The relative expression of the target proteins/β-actin, Columns show mean±SEM (n = 9 for saline exposure; n = 8 for oxytocin exposure). * p<0.05.

Mentions: A total of 811 individual protein spots were visualized across 12 gels (saline: n = 3 males, n = 3 females; oxytocin: n = 3 males, n = 3 females). All spots were included in a partial least squares-discriminant analysis (PLS-DA), which clearly separated protein expression in mice exposed to oxytocin from saline controls (Fig 7A).


The Effect of Oxytocin on Social and Non-Social Behaviour and Striatal Protein Expression in C57BL/6N Mice.

Zhang X, Li Q, Zhang M, Lam S, Sham PC, Bu B, Chua SE, Wang W, McAlonan GM - PLoS ONE (2015)

Striatum proteomics and western blot studies.(A) Multivariate analysis: partial least squares—discriminative analysis (PLS-DA) carried out using SIMCA-P+12.0 software (Umetrics). The striatal protein profile following oxytocin exposure could be clearly differentiated from saline (controls). The XY axis shows the coordinates of the protein distribution in the partial least squares—discriminative analysis (B) Western blot gel images of target proteins. (C) The relative expression of the target proteins/β-actin, Columns show mean±SEM (n = 9 for saline exposure; n = 8 for oxytocin exposure). * p<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696826&req=5

pone.0145638.g007: Striatum proteomics and western blot studies.(A) Multivariate analysis: partial least squares—discriminative analysis (PLS-DA) carried out using SIMCA-P+12.0 software (Umetrics). The striatal protein profile following oxytocin exposure could be clearly differentiated from saline (controls). The XY axis shows the coordinates of the protein distribution in the partial least squares—discriminative analysis (B) Western blot gel images of target proteins. (C) The relative expression of the target proteins/β-actin, Columns show mean±SEM (n = 9 for saline exposure; n = 8 for oxytocin exposure). * p<0.05.
Mentions: A total of 811 individual protein spots were visualized across 12 gels (saline: n = 3 males, n = 3 females; oxytocin: n = 3 males, n = 3 females). All spots were included in a partial least squares-discriminant analysis (PLS-DA), which clearly separated protein expression in mice exposed to oxytocin from saline controls (Fig 7A).

Bottom Line: However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes.With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics.Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), 1095 Jiefang Ave., Wuhan, 430030, P.R. China.

ABSTRACT
Oxytocin has been suggested as a promising new treatment for neurodevelopmental disorders. However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes. Here we investigated the effects of a range of oxytocin doses on social and non-social behaviours in C57BL/6N mice of both sexes. As the striatum modulates social and non-social behaviours, and is implicated in neurodevelopmental disorders, we also conducted a pilot exploration of changes in striatal protein expression elicited by oxytocin. Oxytocin increased prepulse inhibition of startle but attenuated the recognition memory in male C57BL/6N mice. It increased social interaction time and suppressed the amphetamine locomotor response in both sexes. The striatum proteome following oxytocin exposure could be clearly discriminated from saline controls. With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics. The proteins affected by oxytocin could be broadly categorized as those that modulate glutamatergic, GABAergic or dopaminergic signalling and those that mediate cytoskeleton dynamics. Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

Show MeSH
Related in: MedlinePlus