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The Effect of Oxytocin on Social and Non-Social Behaviour and Striatal Protein Expression in C57BL/6N Mice.

Zhang X, Li Q, Zhang M, Lam S, Sham PC, Bu B, Chua SE, Wang W, McAlonan GM - PLoS ONE (2015)

Bottom Line: However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes.With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics.Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), 1095 Jiefang Ave., Wuhan, 430030, P.R. China.

ABSTRACT
Oxytocin has been suggested as a promising new treatment for neurodevelopmental disorders. However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes. Here we investigated the effects of a range of oxytocin doses on social and non-social behaviours in C57BL/6N mice of both sexes. As the striatum modulates social and non-social behaviours, and is implicated in neurodevelopmental disorders, we also conducted a pilot exploration of changes in striatal protein expression elicited by oxytocin. Oxytocin increased prepulse inhibition of startle but attenuated the recognition memory in male C57BL/6N mice. It increased social interaction time and suppressed the amphetamine locomotor response in both sexes. The striatum proteome following oxytocin exposure could be clearly discriminated from saline controls. With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics. The proteins affected by oxytocin could be broadly categorized as those that modulate glutamatergic, GABAergic or dopaminergic signalling and those that mediate cytoskeleton dynamics. Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

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Related in: MedlinePlus

Novel object recognition and social interaction test.(A) 100 μg/kg and 1000 μg/kg oxytocin disrupted recognition memory in males. 1000 μg/kg single exposure (B) and 10 μg/kg and 100 μg/kg repeated exposure to oxytocin (C) increased social interaction in females. (D) 100 μg/kg oxytocin increased social interaction in males. Error bars refer to ± SEM. * post-hoc testing: p<0.05.
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pone.0145638.g005: Novel object recognition and social interaction test.(A) 100 μg/kg and 1000 μg/kg oxytocin disrupted recognition memory in males. 1000 μg/kg single exposure (B) and 10 μg/kg and 100 μg/kg repeated exposure to oxytocin (C) increased social interaction in females. (D) 100 μg/kg oxytocin increased social interaction in males. Error bars refer to ± SEM. * post-hoc testing: p<0.05.

Mentions: Analysis of the novelty discrimination index revealed a significant sex × dose interaction approached significance (F (3, 108) = 2.628, p = 0.054). Post-hoc analyses in each sex separately revealed oxytocin did not alter this index in females but disrupted recognition memory in males (F (3, 54) = 3.869, p = 0.014) at both 100 μg/kg and 1000 μg/kg doses (Bonferroni, (p = 0.023 and p = 0.019 respectively, Fig 5A).


The Effect of Oxytocin on Social and Non-Social Behaviour and Striatal Protein Expression in C57BL/6N Mice.

Zhang X, Li Q, Zhang M, Lam S, Sham PC, Bu B, Chua SE, Wang W, McAlonan GM - PLoS ONE (2015)

Novel object recognition and social interaction test.(A) 100 μg/kg and 1000 μg/kg oxytocin disrupted recognition memory in males. 1000 μg/kg single exposure (B) and 10 μg/kg and 100 μg/kg repeated exposure to oxytocin (C) increased social interaction in females. (D) 100 μg/kg oxytocin increased social interaction in males. Error bars refer to ± SEM. * post-hoc testing: p<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696826&req=5

pone.0145638.g005: Novel object recognition and social interaction test.(A) 100 μg/kg and 1000 μg/kg oxytocin disrupted recognition memory in males. 1000 μg/kg single exposure (B) and 10 μg/kg and 100 μg/kg repeated exposure to oxytocin (C) increased social interaction in females. (D) 100 μg/kg oxytocin increased social interaction in males. Error bars refer to ± SEM. * post-hoc testing: p<0.05.
Mentions: Analysis of the novelty discrimination index revealed a significant sex × dose interaction approached significance (F (3, 108) = 2.628, p = 0.054). Post-hoc analyses in each sex separately revealed oxytocin did not alter this index in females but disrupted recognition memory in males (F (3, 54) = 3.869, p = 0.014) at both 100 μg/kg and 1000 μg/kg doses (Bonferroni, (p = 0.023 and p = 0.019 respectively, Fig 5A).

Bottom Line: However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes.With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics.Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), 1095 Jiefang Ave., Wuhan, 430030, P.R. China.

ABSTRACT
Oxytocin has been suggested as a promising new treatment for neurodevelopmental disorders. However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes. Here we investigated the effects of a range of oxytocin doses on social and non-social behaviours in C57BL/6N mice of both sexes. As the striatum modulates social and non-social behaviours, and is implicated in neurodevelopmental disorders, we also conducted a pilot exploration of changes in striatal protein expression elicited by oxytocin. Oxytocin increased prepulse inhibition of startle but attenuated the recognition memory in male C57BL/6N mice. It increased social interaction time and suppressed the amphetamine locomotor response in both sexes. The striatum proteome following oxytocin exposure could be clearly discriminated from saline controls. With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics. The proteins affected by oxytocin could be broadly categorized as those that modulate glutamatergic, GABAergic or dopaminergic signalling and those that mediate cytoskeleton dynamics. Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

Show MeSH
Related in: MedlinePlus