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The Effect of Oxytocin on Social and Non-Social Behaviour and Striatal Protein Expression in C57BL/6N Mice.

Zhang X, Li Q, Zhang M, Lam S, Sham PC, Bu B, Chua SE, Wang W, McAlonan GM - PLoS ONE (2015)

Bottom Line: However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes.With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics.Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), 1095 Jiefang Ave., Wuhan, 430030, P.R. China.

ABSTRACT
Oxytocin has been suggested as a promising new treatment for neurodevelopmental disorders. However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes. Here we investigated the effects of a range of oxytocin doses on social and non-social behaviours in C57BL/6N mice of both sexes. As the striatum modulates social and non-social behaviours, and is implicated in neurodevelopmental disorders, we also conducted a pilot exploration of changes in striatal protein expression elicited by oxytocin. Oxytocin increased prepulse inhibition of startle but attenuated the recognition memory in male C57BL/6N mice. It increased social interaction time and suppressed the amphetamine locomotor response in both sexes. The striatum proteome following oxytocin exposure could be clearly discriminated from saline controls. With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics. The proteins affected by oxytocin could be broadly categorized as those that modulate glutamatergic, GABAergic or dopaminergic signalling and those that mediate cytoskeleton dynamics. Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

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PPI test.(A) Oxytocin attenuated the baseline startle response to 120dB pulse in female mice. (B) Oxytocin increased the baseline startle response to 100dB pulse in male mice. (C) 100 μg/kg oxytocin attenuated PPI in females. (D) All doses of oxytocin improved PPI in males. Error bars refer to ± SEM.
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pone.0145638.g004: PPI test.(A) Oxytocin attenuated the baseline startle response to 120dB pulse in female mice. (B) Oxytocin increased the baseline startle response to 100dB pulse in male mice. (C) 100 μg/kg oxytocin attenuated PPI in females. (D) All doses of oxytocin improved PPI in males. Error bars refer to ± SEM.

Mentions: Post-hoc analyses in each sex subsequently revealed that oxytocin altered startle responsivity at only one pulse condition in each sex; lowering the startle response at pulse 120 dB stimulus in females (F (3, 54) = 3.330, p = 0.026, Fig 4A); increasing the startle response at pulse 100 dB in male mice (F (3, 54) = 4.157, p = 0.010, Fig 4B). Therefore PPI analyses were conducted in each sex separately and data from pulse 120dB in females and pulse 100dB in males was excluded.


The Effect of Oxytocin on Social and Non-Social Behaviour and Striatal Protein Expression in C57BL/6N Mice.

Zhang X, Li Q, Zhang M, Lam S, Sham PC, Bu B, Chua SE, Wang W, McAlonan GM - PLoS ONE (2015)

PPI test.(A) Oxytocin attenuated the baseline startle response to 120dB pulse in female mice. (B) Oxytocin increased the baseline startle response to 100dB pulse in male mice. (C) 100 μg/kg oxytocin attenuated PPI in females. (D) All doses of oxytocin improved PPI in males. Error bars refer to ± SEM.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696826&req=5

pone.0145638.g004: PPI test.(A) Oxytocin attenuated the baseline startle response to 120dB pulse in female mice. (B) Oxytocin increased the baseline startle response to 100dB pulse in male mice. (C) 100 μg/kg oxytocin attenuated PPI in females. (D) All doses of oxytocin improved PPI in males. Error bars refer to ± SEM.
Mentions: Post-hoc analyses in each sex subsequently revealed that oxytocin altered startle responsivity at only one pulse condition in each sex; lowering the startle response at pulse 120 dB stimulus in females (F (3, 54) = 3.330, p = 0.026, Fig 4A); increasing the startle response at pulse 100 dB in male mice (F (3, 54) = 4.157, p = 0.010, Fig 4B). Therefore PPI analyses were conducted in each sex separately and data from pulse 120dB in females and pulse 100dB in males was excluded.

Bottom Line: However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes.With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics.Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), 1095 Jiefang Ave., Wuhan, 430030, P.R. China.

ABSTRACT
Oxytocin has been suggested as a promising new treatment for neurodevelopmental disorders. However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes. Here we investigated the effects of a range of oxytocin doses on social and non-social behaviours in C57BL/6N mice of both sexes. As the striatum modulates social and non-social behaviours, and is implicated in neurodevelopmental disorders, we also conducted a pilot exploration of changes in striatal protein expression elicited by oxytocin. Oxytocin increased prepulse inhibition of startle but attenuated the recognition memory in male C57BL/6N mice. It increased social interaction time and suppressed the amphetamine locomotor response in both sexes. The striatum proteome following oxytocin exposure could be clearly discriminated from saline controls. With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics. The proteins affected by oxytocin could be broadly categorized as those that modulate glutamatergic, GABAergic or dopaminergic signalling and those that mediate cytoskeleton dynamics. Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

Show MeSH
Related in: MedlinePlus